In Vivo and In Vitro Safety Evaluation of Clinical-grade Cryoprotectant

Zhang Li Juan; Qin Yu Qi; Ren Wei Dong; Dong Jian

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Zhang Li Juan , Qin Yu Qi , Ren Wei Dong , Dong Jian . In Vivo and In Vitro Safety Evaluation of Clinical-grade Cryoprotectant[J]. Journal of Kunming Medical University, 2017, 38(06): 19-24.

Citation:

Zhang Li Juan , Qin Yu Qi , Ren Wei Dong , Dong Jian . In Vivo and In Vitro Safety Evaluation of Clinical-grade Cryoprotectant[J]. Journal of Kunming Medical University, 2017, 38(06): 19-24.

Zhang Li Juan , Qin Yu Qi , Ren Wei Dong , Dong Jian . In Vivo and In Vitro Safety Evaluation of Clinical-grade Cryoprotectant[J]. Journal of Kunming Medical University, 2017, 38(06): 19-24.

Citation:

Zhang Li Juan , Qin Yu Qi , Ren Wei Dong , Dong Jian . In Vivo and In Vitro Safety Evaluation of Clinical-grade Cryoprotectant[J]. Journal of Kunming Medical University, 2017, 38(06): 19-24.

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In Vivo and In Vitro Safety Evaluation of Clinical-grade Cryoprotectant

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基金：云南省科技厅基金资助项目 (2015DG034);

Received Date: 2017-02-14

Abstract

Abstract

Objective To investigate a safe and effective clinical-grade cryoprotectant, which can be infused to patients. Me thods We collected 50 samples of cord blood using hydroxyethyl starch precipitation method to get cord blood nucleated cells. Then we added five kinds of different concentration cryoprectants including DMSO, human serum albumin and Plasmalyte A to cryopreserve nucleated cells in liquid nitrogen. The effects of these cryoprotectants on biological properties of cord blood nucleated cells were monitored and compared after the cells were refrigerated for six months. The detected biological properties included the number of total nucleated cells, CD34+ cells, colony forming units, recovery rate and trypan blue rejection rate. Cryoprotectants of different concentrations were injected into the mice of each group via tail vein, and the liver and renal toxicity were evaluated.Re s ults There was no difference between group A and group C which resulted in higher recovery rates of nucleated cells, CD34+ cells and colony forming units and trypan blue rejection rate than other three groups.The liver toxicity of group A were higher than other groups. Conclus ion The cryoprotectants with 7.5% DMSO +10% human serum albumin+ Plasmalyte A is safe and effective for cryopreserving the nucleated cells.
- Cryoprotectant,
- DMSO,
- Human serum albumin,
- Plasmalyte A,
- Safety

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References(22)

References

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