Establishment and Application An Automatic Typing Method for MAOA μ-VNTR Polymorphism Test using Fluorescence Labeling Techniques

Yao Jin Yong; Hu Li Ping; Nie Ai Ting; Huang Ren Wu; Qiu Jian Bo; Zhang Xiu Feng; Rao 旼; Nie Sheng Jie

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Yao Jin Yong , Hu Li Ping , Nie Ai Ting , Huang Ren Wu , Qiu Jian Bo , Zhang Xiu Feng , Rao 旼, Nie Sheng Jie . Establishment and Application An Automatic Typing Method for MAOA μ-VNTR Polymorphism Test using Fluorescence Labeling Techniques[J]. Journal of Kunming Medical University, 2017, 38(06): 52-55.

Citation:

Yao Jin Yong , Hu Li Ping , Nie Ai Ting , Huang Ren Wu , Qiu Jian Bo , Zhang Xiu Feng , Rao 旼, Nie Sheng Jie . Establishment and Application An Automatic Typing Method for MAOA μ-VNTR Polymorphism Test using Fluorescence Labeling Techniques[J]. Journal of Kunming Medical University, 2017, 38(06): 52-55.

Citation:

Yao Jin Yong , Hu Li Ping , Nie Ai Ting , Huang Ren Wu , Qiu Jian Bo , Zhang Xiu Feng , Rao 旼, Nie Sheng Jie . Establishment and Application An Automatic Typing Method for MAOA μ-VNTR Polymorphism Test using Fluorescence Labeling Techniques[J]. Journal of Kunming Medical University, 2017, 38(06): 52-55.

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Establishment and Application An Automatic Typing Method for MAOA μ-VNTR Polymorphism Test using Fluorescence Labeling Techniques

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基金：国家自然科学基金资助项目 (31660310); 云南省教育厅科学研究基金资助项目 (2015J052);

Received Date: 2017-03-09

Abstract

Abstract

Objective To establish an automatic typing method for MAOA μ-VNTR polymorphism test using capillary electrophoresis and fluorescence labeling techniques. Me thods DNAwas extracted by Chelex-100 method. Blood samples from 720 unrelated Chinese Han individuals in Yunnan were genotyped by capillary electrophoresis and fluorescence labeled primer. Re s ults MAOA μ-VNTR polymorphism fluorescent labeling automatic typing method had high efficiency. And the results from this method were accurate and reliable.Conclus ions The operation of MAOA μ-VNTR polymorphism fluorescent labeling automatic typing method is simple. It has good prospects in the research for the MAOA μ-VNTR polymorphism test.
- MAOA μ-VNTR,
- Polymorphism,
- Fluorescence labeled primers,
- Automatic typing

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References(12)

References

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