Influence of Bone Barrow Stem Cell Transplantation on Immunosuppressed Mice

Qin Yu Qi; Zhang Li Juan; Ren Wei Dong; Dong Jian

Volume 38 Issue 06

Turn off MathJax

Article Contents

Abstract

References

Relative Articles

Article Navigation > Journal of Kunming Medical University > 2017 > 38(06): 9-14

Qin Yu Qi , Zhang Li Juan , Ren Wei Dong , Dong Jian . Influence of Bone Barrow Stem Cell Transplantation on Immunosuppressed Mice[J]. Journal of Kunming Medical University, 2017, 38(06): 9-14.

Citation:

Qin Yu Qi , Zhang Li Juan , Ren Wei Dong , Dong Jian . Influence of Bone Barrow Stem Cell Transplantation on Immunosuppressed Mice[J]. Journal of Kunming Medical University, 2017, 38(06): 9-14.

Qin Yu Qi , Zhang Li Juan , Ren Wei Dong , Dong Jian . Influence of Bone Barrow Stem Cell Transplantation on Immunosuppressed Mice[J]. Journal of Kunming Medical University, 2017, 38(06): 9-14.

Citation:

Qin Yu Qi , Zhang Li Juan , Ren Wei Dong , Dong Jian . Influence of Bone Barrow Stem Cell Transplantation on Immunosuppressed Mice[J]. Journal of Kunming Medical University, 2017, 38(06): 9-14.

PDF( 418 KB)

Influence of Bone Barrow Stem Cell Transplantation on Immunosuppressed Mice

Funds:

基金：云南省科技厅基金资助项目 (2015DG034);

Received Date: 2017-03-12

Abstract

Abstract

Objective To investigate the influence of bone marrow stem cell transplantation on immunosuppressed mice induced by radiotherapy. Me thods Female BALB/C mice were randomly divided into three groups. The mice in test group and in control group were irradiated with X-ray at dosage of 8Gy respectively, while those in blank group were not irradiated. After that, bone marrow stem cells 1x107 in 0.2ml normal saline were injected into mice by tail veins in test group, while 0.2ml normal saline (NS) was injected into mice by tail vein in control group. The peripheral blood was subjected to routine blood test on days 15, 25, 35 after transplantation, T lymphocytes (CD3~+, CD4~+, CD8~+) were determined by flow cytometry (FCM) on days15, 25, 35 after transplantation, and the concentration of mice peripheral blood immunoglobulins Ig M were measured by the ELISA method. All the mice were observed for survivals for 35 days. Re s ults The survival rates of mice 35 d after Hematopoietic stem cell transplantation were 80%, while 10% in control group. The leukocyte counts and Lymph% as well as CD3~+% in peripheral blood after transplantation increased as compared with those received NS (P <0.05) . However, CD4~+%, CD8~+% and serum immunoglobulin Ig M after transplantation decreased, which decreased remarkably on day 15 and begun to recover after that day. BMSC decreased Mono% and CD4~+/CD8~+in peripheral blood (P <0.05) .Conclus ion Bone marrow stem cell transplantation can repair the suppressed immunity of mice.
- Bone marrowstem cells,
- Radiotherapy,
- Mice

FullText(HTML)

References(10)

References

[1]王哲海, 孔莉, 于金明.肿瘤化疗不良反应与对策[M].济南:山东科学技术出版社, 2002:14-18.
[2]徐萌.恶性肿瘤化疗及其[M].北京:军事医学科学院出版社, 2002:31-42.
[3]韩少良, 邵永孚.癌症化学治疗的毒副反应及其处理[M].上海:复旦大学出版社, 2001:85-106.
[4]	[4]VAIDYA A.Hematopoietic Stem Cells, Their Niche, and the Concept of Co-Culture Systems:A Critical Review[J].Journal of stem cells, 2015, 10 (1) :13-31.
[5]	[5]OGONEK J, KRALJ JURIC M, GHIMIRE S, et al.Immune reconstitution after allogeneic hematopoietic stem cell transplantation[J].Frontiers in immunology, 2016, 7 (7) :507.
[6]	[6]MCDERMOTT D F, REGAN M, CLARK J I, et al.Randomized phaseⅢtrial of high-dose interleukin-2 versus subcutaneous interleukin-2 and interferon in patientswith metastatic renal cell carcinoma[J].J Clini Oncol, 2005, 23 (1) :133-141.
[7]	[7]OR R, HADAR E, BITANM.Safety and efficacy of donor lymphocyte in-fusions following mismatched stem cell transplantation[J].Biol Blood Marrow Transplant, 2006, 12 (12) :1295-1301.
[8]	[8]VELA-OJEDA J, GARCA-RUIZ ESPARZA MA, REYES-MALDONADO E, et al.Donor lymphocyte infusions for relapse of chronic myeloid leukemia after allogeneic stem cell transplantation:prognostic significance of the dose of CD3 (+) and CD4 (+) lymphocytes[J].Ann Hematol, 2004, 83 (5) :295-301.
[9]	[9]GLUCKMAN E.Use of hematopoietic stem cells from cord blood for allogenic transplantion in man[J].Bull Acad Natl Med, 1998, 182 (2) :337-348.
[10]	[10]MARTIN P S, LI S, NIKIFOROW S, et al.Infused total nucleated cell dose is a better predictor of transplant outcomes than CD34 (+) cell number in reduced-intensity mobilized peripheral blood allogeneic hematopoietic cell transplantation[J].Haematologica, 2016, 101 (4) :499-505.

Relative Articles(20)

Relative Articles

[1]	Yuxiao LI, Ming HE, Tianrong WANG, Yonggang YAO, Yu WANG. In Vitro Regulation of Th1/Th2 Cell of PBMCs in AR Mice by si-GATA-3. Journal of Kunming Medical University, 2022, 43(8): 7-16. doi: 10.12259/j.issn.2095-610X.S20220802
[2]	Guo-yi LIU, Qing-qing ZHAO, Yan WU, Shan-shan LI, Lian-mei ZHONG, Jia GENG. Effect and Mechanism of Puerarin on Experimental Autoimmune Encephalomyelitis in Mice. Journal of Kunming Medical University, 2021, 42(4): 1-7. doi: 10.12259/j.issn.2095-610X.S20210401
[3]	Zhi-qiang LI, Hong-qiang GAO, Jing-feng LI, Jie-qi YU, Dong-dong WANG, Gang CHEN, Guang CHU. Expression of ICAM-1, MMP-9 and NF-κB in Cholangiocarcinoma and Their Relationship with Immunosuppressive Cells in Peripheral Blood. Journal of Kunming Medical University, 2021, 42(11): 93-98. doi: 10.12259/j.issn.2095-610X.S20211117
[4]	Dong Yuan , Li Cai Xia , Zhao Xian , Tuo Xiao Yu , Liu Liu , Ma Yun . . Journal of Kunming Medical University, 2020, 41(05): 29-32.
[5]	Zhang Xiao Chao , Chen Peng , Shen Zhi Qiang . . Journal of Kunming Medical University, 2019, 40(11): 6-12.
[6]	Ding Su Zhen , Li Lan , Zhu He Fei , Li Shao Min , Dong Jie . . Journal of Kunming Medical University, 2019, 40(12): 34-38.
[7]	Yin Sai Ge , Zhang Heng Rui , Chen Yan , Sun Jun . . Journal of Kunming Medical University, 2019, 40(01): 6-9.
[8]	Zhao Yu , Yuan Jing , Liu Jian Jun , Lu Lin . Comparison of Proliferation between Fetal Forebrain-and Spinal Cord-Derived Neural Stem Cells in Mice. Journal of Kunming Medical University, 2017, 38(09): 19-23.
[9]	Xu Jing Xiao , Cheng Xin Yuan , Guo Shi Yu , Gu Li Hua , Wei Dan Xia , Yang Ren Hua . Effect of Qingre Runzao Oral Liquid on the Immune Function of BALB/C Mice. Journal of Kunming Medical University, 2017, 38(09): 11-14.
[10]	Gao Guo Jie , Shen Shao Ying , Hu Jiang Tian . The Primary Culture of Chondrocytes of Mouse Cranial Base Synchondrosis. Journal of Kunming Medical University, 2017, 38(07): 37-40.
[11]	Xie Shu 姮. Changes and Mechanisms of Immunodepression after Ischemic Stroke in Mice. Journal of Kunming Medical University,
[12]	Du Juan . . Journal of Kunming Medical University, 2015, 36(04): 1-1.
[13]	Wang Yi Yin . . Journal of Kunming Medical University,
[14]	He Ying Hong . . Journal of Kunming Medical University,
[15]	Mu Yun Zhen . . Journal of Kunming Medical University,
[16]	He Ying Hong . . Journal of Kunming Medical University,
[17]	. Establishment of Human Immune Reconstitute NOD/SCID Mice Model. Journal of Kunming Medical University,
[18]	Tai Wen Lin . Anti-inflammatory,Analgesic and Choleretic Effect of Lidan Zhitong Capsulen Mice. Journal of Kunming Medical University,
[19]	. The Effect of Different Carriers on Mice Ovarian Tissue Vitrification. Journal of Kunming Medical University,
[20]	Zhang Yan Li . . Journal of Kunming Medical University,