Li Yan Ming , Zheng Yan Zhen , Zhang Dong Xian , Li Juan , Yang Gen Meng , He Yong Wang , Zhou Yi Qing , Zeng Xiao Feng , Li Zhen . Expression of NMDA Receptor Subunit 2B and iNOS in Rat Striatum by Synergizing Methamphetamine and Human Immunodeficiency Virus Protein Tat[J]. Journal of Kunming Medical University, 2017, 38(07): 1-6.
Citation: Li Yan Ming , Zheng Yan Zhen , Zhang Dong Xian , Li Juan , Yang Gen Meng , He Yong Wang , Zhou Yi Qing , Zeng Xiao Feng , Li Zhen . Expression of NMDA Receptor Subunit 2B and iNOS in Rat Striatum by Synergizing Methamphetamine and Human Immunodeficiency Virus Protein Tat[J]. Journal of Kunming Medical University, 2017, 38(07): 1-6.

Expression of NMDA Receptor Subunit 2B and iNOS in Rat Striatum by Synergizing Methamphetamine and Human Immunodeficiency Virus Protein Tat

Funds:

基金: 国家自然科学基金资助项目 (81560303); 云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2013FB127, 2016FB125); 云南省重大传染病疫苗研发重点实验室开放研究基金资助项目 (KF2015-01); 昆明医科大学科技创新团队建设项目 (CXTD201604);

  • Received Date: 2017-03-24
  • Objectives To observe the NMDA receptor subunit 2B and iNOS expression in rat striatum by injected methamphetamine (MA) and HIV-Tat protein and to explore the synergies of MA and HIV-Tat on neural injury mechanisms. Methods A total of 50 healthy male SD rats were randomly divided into four groups: MA group which received intraperitoneal injection of 10 mg/kg MA by twice per day for 4 days, HIV-Tat group which received intrastriatal injection of 10 μg HIV-Tat, MA + HIV-Tat group which received intraperitoneal injection of MA for 4 days and followed by intrastriatal injection of HIV-Ta) t, and control groups which received intraperitoneal or intrastriatal injection of saline. The rats were killed at 48 h or 7 d after the last time of injection.NMDA receptor subunit 2B and iNOS expression were examined by using immunohistochemistry and western blotting. The results were analyzed and average optical density was measured. Results Compared with those in control group, NMDA receptor subunit 2B and iNOS expression in rat striatum increased in the experimental groups (P <0.05) .Compared with those in MA group or HIV-Tat group, NMDA receptor subunit 2B and iNOS expression increased more significantly in MA + HIV-Tat group.Conclusion Synergies of MA and HIV-Tat protein can dramatically increase the expression of NMDA receptor subunit 2B and iNOS. NMDA acceptor subunit 2B and iNOS can participate in the process of MA and the HIV-Tat protein synergy nerve toxic mechanism.
  • loading
  • [1]
    [1]CHOI H J, YOO T M, CHUNG S Y, et al.Methamphetaine-induced apoptosis in a CNS-derived catecholaminergic cell line[J].Mol Cells, 2002, 13 (2) :221-227.
    [2]
    [2]NAVIA B A, JORDAN B D, PRICE R W.The AIDS dementia complexⅠ.Clinical features[J].Ann Neurol, 1986, 19 (6) :517-524.
    [3]
    [3]WULFF E A, SIMPSON D M.Neuromuscular Complications of HIV-1infection[J].Curr Infect Dis Rep, 1999, 1 (2) :192-197.
    [4]
    [4]ERNST T, CHANG L, JOVICICH J, et al.Abnormal brain activation on functional MRI in cognitively asymptomatic HIV patients[J].Neurology, 2002, 59 (9) :1343-1349.
    [5]
    [5]DABERKOW D P, KESNER R P, KEEFE K A.Relation between metham-phetamine-induced monoamine depletions in the striatum and se-quential motor learning[J].Pharmacol Biochem Behav, 2005, 81 (1) :198-204.
    [6]
    [6]GONZALEZ R, BECHARA A, MARTIN E M.Executive functions among individuals with methamphetamine or alcohol as drugs of choice:preliminary observations[J].J Clin Exp Neuropsychol, 2007, 29 (2) :155-159.
    [7]王彦平, 杨金升.N-甲基-D-天冬氨酸受体NR2B亚基与神经元凋亡[J].国际脑血管病杂志, 2010, 18 (4) :306-310.
    [8]
    [8]CAIS O, SEDLACEK M, HORAK M, et al.Temperature dependence of NR1PNR2B NMDA receptor channels[J].Neuroscience, 2008, 151 (2) :428-438.
    [9]
    [9]LIU Y, WONG T P, AARTS M, et al.NMDA receptor subunits have differential roles in mediating excitotoxic neuronal death both in vitro and in vivo[J].Neurosci, 2007, 27 (11) :2846-2857.
    [10]
    [10]NATHAN C.Nitric oxide as a secretary product of mammalicells[J].FASEB, 1992, 6 (12) :3051-3064.
    [11]
    [11]YAMAMOTO B K, ZHU W.The effects of methamphetamine on the production of free radicals and oxidative stress.[J].Pharmacol Exp Ther, 1998, 287 (1) :107-114.
    [12]
    [12]THOMAS D M, KUHN D M.MK-801 and dextromethorphan block microglial activation and protect against methamphetamine induced neurotoxicity[J].Brain Res, 2005, 1050 (122) :190-198.
    [13]
    [13]FLECKENSTE IN A E, VOLZT, R IDDLE E L, et al.New insights into the mechanism of action of amphetamines[J].Annu Rev Pharmacol Toxicol, 2007, 47 (0) :681-689.
    [14]
    [14]DAVID PUBILL, ANNA M, CANUDAS, et al.Different glial response to methamphetamine-and methylenedioxymethamphetamine-induced neurotoxicity[J].Naunyn-Schmiedeberg's Archives of Pharmacology, 2003, 367 (5) :490-499.
    [15]
    [15]MARAGOS W F, JONES M, BRUCEKELLER A J, et al.Neuronalinjury in hippocampus with human immunodeficiency virustransactivating protein[J].Neuroscience, 2003, 1171 (1) :43-53.
    [16]
    [16]THEODORE S, CASS W A, MARAGOS W F.methamphetamine and human immunodeficiency virus protein tat synergize to destroy dopaminergic termins in the striatum[J]Neuroscience, 2006, 137 (3) :925-935.
  • Relative Articles

    [1] Yuan ZHANG, Tingna ZHU, Yuanyuan CAO, Pengliang LIU, Yihang WANG, Yamei WU, Lihua LI, Yongna ZHAO, Shijun HONG. Effect of TLR4/MyD88/NF- κB Signal Pathway on the Hippocampus of Methamphetamine-dependent CPP Rats. Journal of Kunming Medical University, 2023, 44(2): 7-13.  doi: 10.12259/j.issn.2095-610X.S20230225
    [2] Lin XU, Kaicheng GAO, Jie JIA, Yuyang LI, Huawei WANG, Yiqun KUANG, Yu ZHAO. Effect of Shen-ling-bai-zhu-san on Methamphetamine-Induced Gut Microbiota Change in Mice. Journal of Kunming Medical University, 2023, 44(8): 37-43.  doi: 10.12259/j.issn.2095-610X.S20230829
    [3] Junhong MAO, Danfeng LU, Yu XU, Yicong DAI, Zherui ZHANG, Yue LI, Kunhua WANG. Methamphetamine Alters Methylation Modifications of Synaptic Plasticity Genes in Addicted Mice. Journal of Kunming Medical University, 2022, 43(1): 1-7.  doi: 10.12259/j.issn.2095-610X.S20220121
    [4] Ting-na ZHU, Peng-liang LIU, Wen-juan DONG, Hao YU, Ya-mei WU, Zhao-kui HUANG, Shi-jun HONG, Yong-na ZHAO. Effects of Different Doses of Gastrodin on Conditioned Place Preference and Microglia Activation in Hippocampus of Methamphetamine Dependent Rats. Journal of Kunming Medical University, 2021, 42(5): 6-11.  doi: 10.12259/j.issn.2095-610X.S20210502
    [5] Yuan-yuan LI, Juan LI, Gen-meng YANG, Jian HUANG, Liu LIU, Bao-yu SHEN, Chan WANG, Yue XU, Shu-cheng LIN, Xiao-feng ZENG. Research Progress on Neurotoxic Effects and Mechanism of Methamphetamine. Journal of Kunming Medical University, 2021, 42(2): 153-157.  doi: 10.12259/j.issn.2095-610X.S20210210
    [6] Shen Bao Yu , Yang Gen Meng , Li Yuan Yuan , Liu Liu , Huang Jian , Zeng Xiao Feng , Li Li Hua . . Journal of Kunming Medical University, 2020, 41(01): 163-167.
    [7] Wang Yi Hang , Hong Shi Jun , Zhong Lei Lei , Jie Run Fang , Peng Yan Xia , Li Li Hua . . Journal of Kunming Medical University, 2020, 41(03): 17-21.
    [8] Huang Zhao Kui , Shi Zhen Jin , Wu Ya Mei , Zhou Yi Qing , Chen Li Fang , Wang Yi Hang , Yan Qing Wen , Zhang Feng Yi , Zhao Yong Na , Li Li Hua , Hong Shi Jun . Establishment of the Methamphetamine Induced Rat Conditioned Place Preference Model and the Intervention Effect of Gastrodin. Journal of Kunming Medical University, 2019, 40(03): 1-5.
    [9] Ma Chen Li , Yang Gen Meng , Yan Qing Wen , Zhang Feng Yi , Shi Zhen Jin , Zhou Yi Qing , Wang Yi Hang , Zhao Yong Na , Zhang Dong Xian , Li Li Hua , Hong Shi Jun . Expression of Astrocyte, Neuron, and Proinflammatory Cytokines Derived from Frontal Cortex of Methamphetamine Dependent rats after Gastrodin Administration. Journal of Kunming Medical University, 2018, 39(06): 1-6.
    [10] Yang Jin Feng , Meng Guo Yi , Huang Zhao Kui , Hong Shi Jun , Li Li Hua , Xing Yu Ming , Zhao Yong Na . Effects of Methamphetamine on the Expression of 5-HT, IL-6 and NF-κB in Rats' Hippocampus. Journal of Kunming Medical University, 2018, 39(07): 5-9.
    [11] Sun Yu Xing , Tan Li , Hao Rui , Li Ming , Gan Zheng Yan , Wu Cheng Qing . The Correlation between Exhaled Nitric Oxide and Mycoplasma Pneumoniae Pneumonia in Children. Journal of Kunming Medical University, 2017, 38(09): 79-82.
    [12] Meng Guo Yi , Sui Nian Han , Yang Jin Feng , Bian Li Gong , Hong Shi Jun , Li Li Hua , Zhao Yong Na . . Journal of Kunming Medical University, 2017, 38(02): 19-22.
    [13] Yang Jin Feng , Meng Guo Yi , Sui Nian Han , Bian Li Gong , Hong Shi Jun , Li Li Hua , Zhao Yong Na . Effects of Methamphetamine on the Expression of DA, 5-HT and TNF-α in Rats Hippocampus. Journal of Kunming Medical University, 2017, 38(10): 6-10.
    [14] Tang Tao , Sun Xian Run , Jiang Yan Ping , Zhang Min , Ma Hua , Long Yu . Correlation Between NO/iNOS and Destruction of Articular Cartilage. Journal of Kunming Medical University, 2016, 37(11): 28-32.
    [15] Sui Nian Han . . Journal of Kunming Medical University,
    [16] Zeng Bai Rui . . Journal of Kunming Medical University,
    [17] Yu Yang . . Journal of Kunming Medical University,
    [18] Li Fu Ping . . Journal of Kunming Medical University,
    [19] Shao Xiao Xia . . Journal of Kunming Medical University,
    [20] Liu Ju Peng . New Development in Role of BDNF in the Mechanism of Methamphetamine Dependence. Journal of Kunming Medical University,
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2014) PDF downloads(97) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return