Expression of NMDA Receptor Subunit 2B and iNOS in Rat Striatum by Synergizing Methamphetamine and Human Immunodeficiency Virus Protein Tat

Li Yan Ming; Zheng Yan Zhen; Zhang Dong Xian; Li Juan; Yang Gen Meng; He Yong Wang; Zhou Yi Qing; Zeng Xiao Feng; Li Zhen

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Li Yan Ming , Zheng Yan Zhen , Zhang Dong Xian , Li Juan , Yang Gen Meng , He Yong Wang , Zhou Yi Qing , Zeng Xiao Feng , Li Zhen . Expression of NMDA Receptor Subunit 2B and iNOS in Rat Striatum by Synergizing Methamphetamine and Human Immunodeficiency Virus Protein Tat[J]. Journal of Kunming Medical University, 2017, 38(07): 1-6.

Citation:

Li Yan Ming , Zheng Yan Zhen , Zhang Dong Xian , Li Juan , Yang Gen Meng , He Yong Wang , Zhou Yi Qing , Zeng Xiao Feng , Li Zhen . Expression of NMDA Receptor Subunit 2B and iNOS in Rat Striatum by Synergizing Methamphetamine and Human Immunodeficiency Virus Protein Tat[J]. Journal of Kunming Medical University, 2017, 38(07): 1-6.

Citation:

Li Yan Ming , Zheng Yan Zhen , Zhang Dong Xian , Li Juan , Yang Gen Meng , He Yong Wang , Zhou Yi Qing , Zeng Xiao Feng , Li Zhen . Expression of NMDA Receptor Subunit 2B and iNOS in Rat Striatum by Synergizing Methamphetamine and Human Immunodeficiency Virus Protein Tat[J]. Journal of Kunming Medical University, 2017, 38(07): 1-6.

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Expression of NMDA Receptor Subunit 2B and iNOS in Rat Striatum by Synergizing Methamphetamine and Human Immunodeficiency Virus Protein Tat

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基金：国家自然科学基金资助项目 (81560303); 云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2013FB127, 2016FB125); 云南省重大传染病疫苗研发重点实验室开放研究基金资助项目 (KF2015-01); 昆明医科大学科技创新团队建设项目 (CXTD201604);

Received Date: 2017-03-24

Abstract

Abstract

Objectives To observe the NMDA receptor subunit 2B and iNOS expression in rat striatum by injected methamphetamine (MA) and HIV-Tat protein and to explore the synergies of MA and HIV-Tat on neural injury mechanisms. Methods A total of 50 healthy male SD rats were randomly divided into four groups: MA group which received intraperitoneal injection of 10 mg/kg MA by twice per day for 4 days, HIV-Tat group which received intrastriatal injection of 10 μg HIV-Tat, MA + HIV-Tat group which received intraperitoneal injection of MA for 4 days and followed by intrastriatal injection of HIV-Ta) t, and control groups which received intraperitoneal or intrastriatal injection of saline. The rats were killed at 48 h or 7 d after the last time of injection.NMDA receptor subunit 2B and iNOS expression were examined by using immunohistochemistry and western blotting. The results were analyzed and average optical density was measured. Results Compared with those in control group, NMDA receptor subunit 2B and iNOS expression in rat striatum increased in the experimental groups (P <0.05) .Compared with those in MA group or HIV-Tat group, NMDA receptor subunit 2B and iNOS expression increased more significantly in MA + HIV-Tat group.Conclusion Synergies of MA and HIV-Tat protein can dramatically increase the expression of NMDA receptor subunit 2B and iNOS. NMDA acceptor subunit 2B and iNOS can participate in the process of MA and the HIV-Tat protein synergy nerve toxic mechanism.
- Methamphetamine,
- Tat protein,
- NMDA receptor subunit 2B,
- iNOS,
- Neurotoxicity

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References(16)

References

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