Expression of Survivin in Circulating Tumor Cells in Patients with Bladder Cancer

Yan Yong; Sun Xuan; Li Gang; Zheng Cuan; Ma Shao Xiang; Li Yong; Zhao Run Heng; Wang Ke

Article Contents

Article Navigation > Journal of Kunming Medical University > 2017 > 38(07): 102-106

Yan Yong , Sun Xuan , Li Gang , Zheng Cuan , Ma Shao Xiang , Li Yong , Zhao Run Heng , Wang Ke . Expression of Survivin in Circulating Tumor Cells in Patients with Bladder Cancer[J]. Journal of Kunming Medical University, 2017, 38(07): 102-106.

Citation:

Yan Yong , Sun Xuan , Li Gang , Zheng Cuan , Ma Shao Xiang , Li Yong , Zhao Run Heng , Wang Ke . Expression of Survivin in Circulating Tumor Cells in Patients with Bladder Cancer[J]. Journal of Kunming Medical University, 2017, 38(07): 102-106.

Citation:

PDF( 202 KB)

Expression of Survivin in Circulating Tumor Cells in Patients with Bladder Cancer

Funds:

基金：昆明市科技局科研基金资助项目 (2015-2-S-01577);

Received Date: 2017-04-19

Abstract

Abstract

Objective To investigate the expression of Survivin in circulating tumor cells (CTC) and the association between CTC and clinicopathologic characteristics of bladder cancer. Methods Clinically confirmed 50 bladder cancer patients were enrolled in the study. Can Patrol technology was applied to determine different types of CTC. And expressions of Survivin in these CTC were detected by immunohistochemical procedures. C2 test and multi-variates Logistic regression were employed to determine the association between Survivin in CTC and clinicopathologic characteristics of tumors. Results The positive rate of CTC in bladder cancer was 42% (21/50) .The mesenchymal type CTC had the most expression of Survivin (90.9%, with P<0.05) . The presence of CTC and positive expression of Survivin were correlated with tumor size, number of tumor, pathological stage, and pathological grade (P<0.05) . Multivariate analysis confirmed that the presences of CTC and positive expression of Survivin were significant correlated with tumor size, pathological stage, and pathological grade (P <0.05) .Conclusion The presence of CTC and expression of Survivin may suggest the re-occurrence, progression and metastasis of bladder tumor.
- Bladder neoplasms,
- Circulating tumor cells,
- Survivin

FullText(HTML)

References(25)

References

[1]	[1]ZENG H, ZHENG R, GUO Y, et al.Cancer survival in China, 2003-2005:A population-based study[J].Int J Cancer, 2015, 136 (8) :1921-1930.
[2]	[2]TORRE L A, BRAY F, SIEGEL R L, et al.Global cancer statistics, 2012[J].CA Cancer J Clin, 2015, 65 (2) :87-108.
[3]	[3]RINK M, CHUN F K, DAHLEM R, et al.Prognostic role and HER 2expression of circulating tumor cells in peripheral blood of patients prior to radical cystectomy:A prospective study[J].Eur Urol, 2012, 61 (4) :810-817.
[4]	[4]VOLKMER B G, KUEFER R, BARTSCH G C, et al.Oncological followup after radical cystectomy for bladder cancer is there any benefit[J].J Urol, 2009, 181 (4) :1587-1593.
[5]	[5]TURKER I, UYETURK U, SONMEZ O U, et al.Detection of circulating tumor cells in breast cancer patients:prognostic predictive role[J].Asian Pac J Cancer Prev, 2013, 14 (3) :1601-1607.
[6]	[6] DU Y J, LI J, ZHU W F, et al.Survivin m RNA-circulating tumor cells Predict treatment efficacy of chemotherapy and survival for advanced non-small cell lung cancer patients[J].Tumour Biol, 2014, 35 (5) :4499-4507.
[7]	[7] HU B, ROCHEFORT H, GOLDKOM A.Circulating tumor cells in prostate cancer[J].Cancers (Basel) , 2013, 5 (4) :1676-1690.
[8]	[8]KANIA J, KONTMK S J, MARLICZ K, et al.Expression of survivin and caspase-3 in gastric cancer[J].Dig Dis Sci, 2003, 48 (2) :266-271.
[9]	[9]SAUTER G, ALGABA F, AMIN M, et al.Tumour of urinary system:non-invasive urothelial neoplasia[M]In Eble JN, Sauter G, Epstein JI, Sesterhenn, eds.WHO Classification of Tumour of urinary system and male genital organs.Lyon:IARCC Press, 2004:29-34.
[10]	[10]NEZOS A, PISSIMISIS N, LEMBESSIS P, et al.Detection of circulating tumor cells in bladder cancer patients[J].Cancer Treat Rev, 2009, 35 (3) :272-279.
[11]刘侃, 张俊哲.基于微流控芯片的循环肿瘤细胞的分离、检测技术[J].电脑知识与技术, 2014, 10 (9) :2093-2097.
[12]	[12]HONG B, ZU Y.Detecting circulating tumor cells:current challenges and new trends[J].Theranostics, 2013, 3 (6) :377-394.
[13]	[13]PARKINSON D R, DRACOPOLI N, PETTY B G, et al.Considerations in the development of circulating tumor cell technology for clinical use[J].J Transl Med, 2012, 10 (1) :138-157.
[14]	[14]BEDNARZ-KNOLL N, ALIX-PANABI6RES C, PANTEL K.Plasticity of disseminating cancer cells in patients with epithelial malignancies[J].Cancer Metastasis Rev, 2012, 31 (3) :673-687.
[15]	[15]MIN Y, ADITYA B, BEN S, et al.Circulating breast tumor cells exhibit dynamic changes in epithelial and mesenchymal composition[J].Science, 2013, 339 (6119) :580-584.
[16]	[16]DU Y J, LI J, ZHU W F, et al.Survivin m RNA-circulating tumor cells Predict treatment efficacy of chemotherapy and survival for advanced non-small cell lung cancer patients[J].Tumour Biol, 2014, 35 (5) :4499-4507.
[17]	[17]MikulováV, CabiňakováM, JanatkováI, et a1.Detection of circulating tumor cells during follow-up of patients with early breast cancer:clinical utility for monitoring of therapy efficacy[J].Scand J Clin Lab Invest, 2014, 74 (2) :132-142.
[18]	[18]JUDONG YANG, MICHAEL B, CAMPION, et al.Circulating tumor cells are associated with poor overall survival in patients with cholangiocarcinoma[J].Hepatology, 2016, 1 (63) :148-158.
[19]	[19]HU B, ROCHEFORT H, GOLDKOM A.Circulating tumor cells in prostate cancer[J].Cancers (Basel) , 2013, 5 (4) :1676-1690.
[20]	[20]KEE-THAI K, THOMAS H, HSIAO-YEN H, et al.Expression of survivin in bladder cancer cell lines using quantitative real-time polymerase chain reaction[J].Urol Sci, 2013, 15 (2) :2348-2350.
[21]	[21]LEHNER R, LUCIA M S, JARBOE E A, et al.Immunohistochemical localization of the IAP protein survivin in bladder mucosa and transitional cell carcinoma[J].Appl Immunohistochem Mol Morphol, 2002, 10 (2) :134-138.
[22]	[22]PLAKS V, KOOPMAN C D, WERB Z.Cancer.Circulating tumor cells[J].Science, 2013, 341 (6151) :1186-1188.
[23]	[23]GAZZANIGA P, GRADILONE A, DE B E, et al.Prognostic value of circulating tumor cells in nonmuscle invasive bladder cancer:a Cell Search analysis[J].Annals of Oncology, 2012, 23 (1) :2352-2356.
[24]	[24]GRADILONE A, PETRACCA A, NICOLAZZO C, et al.Prognostic significance of survivin-expressing circulating tumour cells in T1G3 bladder cancer[J].Bju International, 2010, 106 (5) :710-715.
[25]	[25]SHIYANG W U, ZHIMING L I U, SUYAN LI U, et al.Enrichment and enumeration of circulating tumor cells by efficient depletion of leukocyte fractions[J].Clin Chem Lab Med, 2014, 52 (23) :243-251.

Relative Articles(20)

Relative Articles

[1]	Qi Xiao , Zhong Zhao Ming , Sun Chuan Zheng . . Journal of Kunming Medical University, 2020, 41(05): 140-144.
[2]	Li Hui , Ping Qin Rong , Shi Yun Qiang , Wang Chun Hui , Yang Yang , Hu Li Bing , Bi Xiao Fang , Li Jian , Zhong Yi Ming . . Journal of Kunming Medical University, 2020, 41(01): 52-56.
[3]	Huang Yu , Luan Ting , Wang Hai Feng , Wang Jian Song . . Journal of Kunming Medical University, 2019, 40(11): 147-151.
[4]	Zhang Jian Hua , Shen Zheng Chao , Chen Yin , Gu Peng , Zhao Hui , Shen Ji Hong , Ke Kun Bin . . Journal of Kunming Medical University, 2019, 40(11): 112-115.
[5]	Ran Zhen , Zhu Yun Sha , Hai Bing , Zhang Jing Song . Relationship between the Proportion of Tumor Infiltrating Lymphocytes in Tumor Mass and Postoperative Recurrence of Prostate Carcinoma. Journal of Kunming Medical University, 2018, 39(03): 62-66.
[6]	Hu Zhu Fei , He Yong Wen . . Journal of Kunming Medical University, 2017, 38(05): 133-137.
[7]	Jie Yan Hua . . Journal of Kunming Medical University,
[8]	Zhang Jing Song . . Journal of Kunming Medical University,
[9]	Zhan Hui . . Journal of Kunming Medical University,
[10]	Hu Li Bing . . Journal of Kunming Medical University,
[11]	Sun Zhi Min . . Journal of Kunming Medical University,
[12]	Li Rui . . Journal of Kunming Medical University,
[13]	Wei Xiang Qun . . Journal of Kunming Medical University,
[14]	Ma Zhen . . Journal of Kunming Medical University, 2012, 33(07): 1-1.
[15]	Jie Yan Hua . Chest X-ray Radiographic and CT Findings in Severe A (H1N1)Virus Infection. Journal of Kunming Medical University,
[16]	Ai Yi Qin . Focal Nodular Hyperplasia of Liver:A Report of 12 Cases. Journal of Kunming Medical University,
[17]	. The Cause Analysis on the Failure of Tumor Cells Culture from Malignant Hydrothorax. Journal of Kunming Medical University,
[18]	Li Kun Lun . Effect of Survivin Gene Expression Inhibition by RNAi on Breast Cancer SKBr-3 Cells. Journal of Kunming Medical University,
[19]	. Expression of Survivin in Differentiated Thyroid Carcinomas and Its Clinical Significance. Journal of Kunming Medical University,
[20]	. Clinical Research of the Expression and Correlation of KI-67 and Survivin in Cervical Carcinoma. Journal of Kunming Medical University,