Changes in Hippocampus Synapses Caused by Long-term Depression in Old Rats with Neuropathic Pain

Wei Hui Ming; Ma Wei Qing; Dong Fa Tuan; Li Wen Feng; Pu Jun Jie; He Yan Feng

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Article Navigation > Journal of Kunming Medical University > 2017 > 38(07): 41-44

Wei Hui Ming , Ma Wei Qing , Dong Fa Tuan , Li Wen Feng , Pu Jun Jie , He Yan Feng . Changes in Hippocampus Synapses Caused by Long-term Depression in Old Rats with Neuropathic Pain[J]. Journal of Kunming Medical University, 2017, 38(07): 41-44.

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Wei Hui Ming , Ma Wei Qing , Dong Fa Tuan , Li Wen Feng , Pu Jun Jie , He Yan Feng . Changes in Hippocampus Synapses Caused by Long-term Depression in Old Rats with Neuropathic Pain[J]. Journal of Kunming Medical University, 2017, 38(07): 41-44.

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Changes in Hippocampus Synapses Caused by Long-term Depression in Old Rats with Neuropathic Pain

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基金：成都军区“十二五”医学科研项目 (B14013);

Received Date: 2017-03-12

Abstract

Abstract

Objective To investigate the changes in Hippocampus synapses caused by long-term depression (LTD) in old rats with neuropathic pain. Method Fifteen old male Wistar rats were randomly divided into 3 groups (n = 5 each) : sham operating group (group S) , neuropathic pain group (group NP) , and NP + AP5 group (group NA) . Neuropathic pain was produced by ligation of the right L4, L5 spinal nerve. The behaviour of the rats was evaluated. The pain threshold was measured by electric stimulation at 7, 14 and 21 d after surgery. The excitatory post-synaptic potential (EPSP) in hippocampal CA1 region was recorded at 3 d after the last measurement of pain threshold. The changes in EPSP and input/output curve and LTD induced by low frequency stimulation (LFS) of the CA1 stratum radiatum of the rat hippocampus were observed using stereotaxic technology and extracellular recording respectively. Group NA received infusion of AP5 (NMDA receptor antagonist) 6 μl by i.c.v 20 min before LFS. Results The pain threshold was significantly lower in group NP and NA than in group S.The amplitude of LTD was significantly lower in group NP than in group S and NA (P<0.05) , but there was no significant difference in the amplitude of LTD between group S and NA (P>0.05) . Conclusion The results suggest that the neuropathic pain by ligation of the right L4, L5 spinal nerve might facilitate LTD induction in the CA1 area of the hippocampus of old rats; mechanism might be related to the activation of NMDA receptors.
- Neuralgia,
- Long-term depression,
- Hippocampus,
- Rat,
- Aged

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References(15)

References

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