Distribution of Metaldehyde in Some Tissues of Subacutely Poisoned Rats

Li Hong; Zheng Hong Fu; Jie Run Fang; Li Shu Hua; Zhang Rui Lin; Wang Rui; Li Hui Fang Jie

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Li Hong , Zheng Hong Fu , Jie Run Fang , Li Shu Hua , Zhang Rui Lin , Wang Rui , Li Hui Fang Jie . Distribution of Metaldehyde in Some Tissues of Subacutely Poisoned Rats[J]. Journal of Kunming Medical University, 2017, 38(07): 7-11.

Citation:

Li Hong , Zheng Hong Fu , Jie Run Fang , Li Shu Hua , Zhang Rui Lin , Wang Rui , Li Hui Fang Jie . Distribution of Metaldehyde in Some Tissues of Subacutely Poisoned Rats[J]. Journal of Kunming Medical University, 2017, 38(07): 7-11.

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Distribution of Metaldehyde in Some Tissues of Subacutely Poisoned Rats

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基金：国家自然科学基金资助项目 (81460213); 云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2014FB007);

Received Date: 2017-04-21

Abstract

Abstract

Objective To develop the method for the determination of metaldehyde in biological material by gas chromatography/mass spectrometry (GC/MS) and GC-FID and to investigate the distribution of metaldehyde in main tissues of subacutely poisoned rats.Methods SD rats were given the oral administration of metaldehyde (40mg/kg, 140 mg/kg, 280 mg/kg) and executed by hypoxia after one week. Cardiac blood, liver, stomach and kidney were taken out and the contents of metaldehyde were detected. The rats in control group were given the oral administration of physiologic saline, and their blood and tissues were taken out correspondingly to be used as blank.The blood and tissues samples were extracted by dichloromethane. The metaldehyde was qualitatively analyzed by GC/MS and quantitatively analyzed by GC-FID.Results A GC/MS and GC-FID method for the determination of metaldehyde in biological samples was established. The limit of detection of GC/MS was 0.1ng while the GC-FID method displayed a satisfactory linearity within the concentration ranges of 0.010.1 mg/mL (R=0.9992) . The average recovery was over 75%.Conclusion The order of metaldehyde content in blood and tissues is: gastric tissues>cardiac blood>kidney>liver.This method is simple, quick, accurate, replicated and suitable for the determination of metaldehyde in biological samples.
- Metaldehyde,
- Biological samples,
- Gas chromatography/mass spectrometry,
- Gas chromatography,
- Distribution

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References(15)

References

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