Effects of Corilagin on Expression of CRP in OX-LDL Injured Macrophage Cells

Zhang Xiao Chao; Shen Zhi Qiang; Yang Ren Hua; Tang Chuan Jing; Bai Lin Ying; He Bo; Chen Peng; Yao Rong Cheng

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Zhang Xiao Chao , Shen Zhi Qiang , Yang Ren Hua , Tang Chuan Jing , Bai Lin Ying , He Bo , Chen Peng , Yao Rong Cheng . Effects of Corilagin on Expression of CRP in OX-LDL Injured Macrophage Cells[J]. Journal of Kunming Medical University, 2017, 38(08): 1-5.

Citation:

Zhang Xiao Chao , Shen Zhi Qiang , Yang Ren Hua , Tang Chuan Jing , Bai Lin Ying , He Bo , Chen Peng , Yao Rong Cheng . Effects of Corilagin on Expression of CRP in OX-LDL Injured Macrophage Cells[J]. Journal of Kunming Medical University, 2017, 38(08): 1-5.

Citation:

Zhang Xiao Chao , Shen Zhi Qiang , Yang Ren Hua , Tang Chuan Jing , Bai Lin Ying , He Bo , Chen Peng , Yao Rong Cheng . Effects of Corilagin on Expression of CRP in OX-LDL Injured Macrophage Cells[J]. Journal of Kunming Medical University, 2017, 38(08): 1-5.

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Effects of Corilagin on Expression of CRP in OX-LDL Injured Macrophage Cells

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基金：国家自然科学基金资助项目 (81260493, 81160401, 81660613); 云南省社会发展计划重点基金资助项目 (2015FA021, 2012FA002); 云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2013FB103, 2012FB024); 云南省教育厅科学研究基金资助项目 (2015Y154);

Received Date: 2017-05-10

Abstract

Abstract

Objective To investigate the effects of Corilagin on expression of C-reactive protein (CRP) in oxidized low density lipoprotein ( OX-LDL) injured macrophage cells. Methods In vitro, mice monocyte-derived macrophages were prepared to duplicate the injury model caused by OX-LDL; Western blotting essay was used to determine the effect of Corilagin on CRP protein expression and Real time PCR assay was used to study the CRP m RNA expression, respectively. Results Compared with normal control group, the CRP/β-actin and 2-ΔΔCtvalues of OX-LDL injured model group were markedly increased (P<0.05) ;compared with the model group, Corilagin at 3.125-50 μmol/L obviously decreased the values of CRP/β-actin and 2-ΔΔCt (P<0.05) . Conclusion Corilagin could significantly down-regulate protein and m RNA expression of CRP in OX-LDL injured macrophage cells.
- Corilagin,
- Atherosclerosis,
- Macrophage cells,
- C-reactive protein

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References(17)

References

[1]	[1]BUTOI E, GAN A M, TUCUREANU M M, et al.Cross-talk between macrophages and smooth muscle cells impairs collagen and metalloprotease synthesis and promotes angiogenesis[J].Biochim Biophys Acta, 2016, 1863 (7) :1568-1578.
[2]张文美, 贾立昕, 李涛涛, 等.巨噬细胞分化在心肌梗死后心脏重构中的作用研究[J].中国药物与临床, 2014, 14 (1) :1-4.
[3]白书玲, 李建军.C反应蛋白与动脉粥样硬化[J].中华心血管病杂志, 2004, 32 (8) :765-768.
[4]	[4]RIDLER P M, HENEKERS C H, BURING J E, et al.C-reactive protein and other markers of inflammation in the prediction of cardio vascular disease in w omen[J].N Engl J Med, 2000, 342 (12) :836-843.
[5]程艳刚, 裴妙荣, 孔祥鹏, 等.叶下珠化学成分和药理作用研究进展[J].辽宁中医药大学学报, 2016, 18 (4) :238-242.
[6]刘韬, 张悦, 杨红玲, 等.Corilagin对HUVEC增殖及细胞周期的影响[J].昆明医科大学学报, 2014, 35 (12) :7-11.
[7]	[7]FUSTER V, BADIMON L, BADIMON J J, et al.The pathogenesis of coronary artery disease and the acute coronary syndromes (1-2) [J].N Engl J Med, 1992, 326 (4) :242-250.
[8]	[8]PASTERKAMP G, DEN RUIJTER H M, LIBBY P.Temporal shifts in clinical presentation and underlying mechanisms of atherosclerotic disease[J].Nat Rev Cardiol, 2017, 14 (1) :21-29.
[9]王辉, 历淑娟.动脉粥样硬化易损斑块分子机制的研究进展[J].河北联合大学学报, 2013, 15 (1) :32-34.
[10]陈莎莎, 李新明.巨噬细胞、血管平滑肌细胞与冠状动脉粥样硬化易损斑块的相关性研究[J].临床内科杂志, 2012, 29 (7) :502-504.
[11]	[11]PAKALA R, LEBORGNE L, CHENEAU E, et al.Radiation-induced atherosclerotic plaque progression in a hypercholesterolemic rabbit:a prospective vulnerable plaque model[J].Cardiovasc Radiat Med, 2003, 4 (3) :146-151.
[12]胡先同, 马东明, 高青, 等.巨噬细胞及其相关因子在家兔不稳定斑块中的作用及其机制[J].天津中医药大学学报, 2014, 33 (2) :83-86.
[13]	[13]FEIG J E, PARATHATH S, RONG J X, et al.Reversal of hyperlipidemia with a genetic switch favorably affects the content and inflammatory state of macrophages in atherosclerotic plaques[J].Circulation, 2011, 123 (9) :989-998.
[14]	[14]ZWAKA T P, HOMBACH V, TORZEWSKI J.C-reactive protein-mediated low density lipoprotein uptake by macrophages:implications for atherosclerosis[J].Circulation, 2001, 10 (3) :1194-1197.
[15]魏亚芬, 殷萍, 张宇, 等.急性脑梗死患者动脉粥样硬化程度与C反应蛋白的相关性研究[J].中国急救医学, 2008, 28 (11) :1021-1023.
[16]	[16]YASOJIMA K, SCHWAB C, MCGEER E G, et al.Generation of C-reactive protein and complement components in atherosclerotic plagues[J].Am J Pathol, 2001, 158 (3) :1039-1051.
[17]	[17]PAUL A, KO KW, LI L, et al.C-reactive protein accelerates the progression of atherosclerosis in apolipoprotein E deficient mice[J].Circulation, 2004, 109 (5) :647-655.

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