Isolation and Antibacterial Activity of Fungal Endophytes in Paris polyphylla Smith var. yunnanenesis

Long Xi Cui; Zhong Jie Shi; Wu Yi; Xuan Qun; Zhang Cai Jun; Liu Yun Xia; Pan Hong Mei; Guo Ying

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Long Xi Cui , Zhong Jie Shi , Wu Yi , Xuan Qun , Zhang Cai Jun , Liu Yun Xia , Pan Hong Mei , Guo Ying . Isolation and Antibacterial Activity of Fungal Endophytes in Paris polyphylla Smith var. yunnanenesis[J]. Journal of Kunming Medical University, 2017, 38(08): 16-19.

Citation:

Long Xi Cui , Zhong Jie Shi , Wu Yi , Xuan Qun , Zhang Cai Jun , Liu Yun Xia , Pan Hong Mei , Guo Ying . Isolation and Antibacterial Activity of Fungal Endophytes in Paris polyphylla Smith var. yunnanenesis[J]. Journal of Kunming Medical University, 2017, 38(08): 16-19.

Citation:

Long Xi Cui , Zhong Jie Shi , Wu Yi , Xuan Qun , Zhang Cai Jun , Liu Yun Xia , Pan Hong Mei , Guo Ying . Isolation and Antibacterial Activity of Fungal Endophytes in Paris polyphylla Smith var. yunnanenesis[J]. Journal of Kunming Medical University, 2017, 38(08): 16-19.

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Isolation and Antibacterial Activity of Fungal Endophytes in Paris polyphylla Smith var. yunnanenesis

Funds:

基金：云南省应用基础研究基金资助项目 (2014FB017); 昆明医科大学本科生创新研究基金资助项目 (J121011006);

Received Date: 2017-04-01

Abstract

Abstract

Objective To isolate the fungal endophytes from Paris Polyphylla Smith var.yunnanensis and investigate the antibacterial activities of these fungi to human's pathogenic bacteria. Methods After surface sterilization, fungal endophytes were cultivated on yeast powder dextrosed (YPD) .The fermentation liquid of every fungal strain was extracted by ethyl acetate. Afterwards, the effect of antibacterial activity on Staphylococcus aureus, Salmonella typhi and Endococcus faecium was investigated by paper disk test. Results54 strains of fungal endophytes were isolated and purified. Among them, 20 strains had antibacterial activities, which accounted for 37% of the total isolated strains. Inhibition zones of J09 metabolites to the three indicators was ( 49.67 ±1.25) mm, ( 50.33 ±0.52) mm, ( 37.00 ±1.63) mm respectively. In terms of the morphological and molecular biological characteristics, J09 was identified as Penicillium griseofulvum.Meanwhile, the antibacterial compounds were not penicillin or griseofulvin by high performance liquid chromatography (HPLC) detection. Conclusion The fungal endophyte J09 could be a potential candidate to develop an antibacterial medicine since its metabolites have showed strong antibacterial activities.
- Paris Polyphylla Smith var,
- yunnanenesis,
- Fungal endophytes,
- Antimicrobial activity,
- Penicillium griseofulvum,
- Isolation and Identification

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References(14)

References

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