Significance of Thyroid Transcription Factor-1 and Phosphatase and Tensin Homolog Genes Expression in Early Endometrial Cancer

Lu Xiao Dong; Lu Fang Li; Duan Zhao; Du Lian Jiang

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Lu Xiao Dong , Lu Fang Li , Duan Zhao , Du Lian Jiang . Significance of Thyroid Transcription Factor-1 and Phosphatase and Tensin Homolog Genes Expression in Early Endometrial Cancer[J]. Journal of Kunming Medical University, 2017, 38(08): 62-66.

Citation:

Lu Xiao Dong , Lu Fang Li , Duan Zhao , Du Lian Jiang . Significance of Thyroid Transcription Factor-1 and Phosphatase and Tensin Homolog Genes Expression in Early Endometrial Cancer[J]. Journal of Kunming Medical University, 2017, 38(08): 62-66.

Citation:

Lu Xiao Dong , Lu Fang Li , Duan Zhao , Du Lian Jiang . Significance of Thyroid Transcription Factor-1 and Phosphatase and Tensin Homolog Genes Expression in Early Endometrial Cancer[J]. Journal of Kunming Medical University, 2017, 38(08): 62-66.

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Significance of Thyroid Transcription Factor-1 and Phosphatase and Tensin Homolog Genes Expression in Early Endometrial Cancer

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基金：陕西省自然科学基金资助项目 (2013JM4012);

Received Date: 2017-04-07

Abstract

Abstract

Objective To study the significance of thyroid transcription factor-1 (TTF-1) and phosphatase and tensin homolog (PTEN) genes expression in early endometrial cancer. Methods A total of 41 consecutives as endometrial cancer, 38 patients with hyperplastic endometrium and 13 cases of normal endometrium were enrolled, then we detected the levels of TTF-1 and PTEN mRNA in the three endometrium tissues by fluorescent quantitative PCR (RT-PCR) , and analyzed the relationship between them and clinicopathological features;the correlation of TTF-1 and PTEN mRNA to mi R-135 b, mi R-125 b and Snail mRNA levels in the three endometrium tissues by RT-PCR. Results The levels of TTF-1 and PTEN mRNA in endometrial cancer tissue were both significantly lower than the other two, and were the highest in normal endometrium (P<0.05) .The expressions of TTF-1 and PTEN were no relationship to menopause, adenocarcinoma or squamous cell carcinoma (P >0.05) ; while they were lower as FIGO classes, myometrial invasion and pelvic lymph node metastasis were increasing (P <0.05) .The level of mi R-135 b mRNA in endometrial cancer tissue was significantly higher, while mi R-125 b and Snail mRNA were lower than the other two, mi R-135 b mRNA level in normal endometrium was the lowest, mi R-125 b and Snail mRNA was the highest (P<0.05) .The TTF-1 and PTEN mRNA expressions were positively ralted to mi R-135 b mRNA level, while negatively related to mi R-125 b and Snail mRNA.The diagnosed sensitivity of TTF-1 mRNA for endometrial cancer was 86.5%, specificity84.2%, accuracy 0.823, 95% CI=0.7620.921, P=0.012 by ROC model;while they were 85.3%, 83.6%, 0.842, 95% CI=0.7850.936, P=0.010 of PTEN mRNA correspondingly. Conclusion TTF-1 and PTEN genes can be the molecular markers for early diagnosis of endometrial cancer, are related to clinical features, and may influence tumor by regulating tumor cell proliferation activity.
- Endometrial cancer,
- Thyroid transcription factor-1,
- Phosphatase and tensin homolog,
- miR-135b,
- miR-125b,
- Snail

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References(19)

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