Effect of Yunnan Baiyao on the Secretion of TNF-α, IL-6, IL-1β from Human Dental Pulp Cells under LPS-PG induced Inflammatory Environment

Huang Song Jing; Lei Ya Yan; She Rui; Wu Jian Hua; Liu Shu Mei; Zhang Ming Zhu

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Huang Song Jing , Lei Ya Yan , She Rui , Wu Jian Hua , Liu Shu Mei , Zhang Ming Zhu . Effect of Yunnan Baiyao on the Secretion of TNF-α, IL-6, IL-1β from Human Dental Pulp Cells under LPS-PG induced Inflammatory Environment[J]. Journal of Kunming Medical University, 2017, 38(08): 73-78.

Citation:

Huang Song Jing , Lei Ya Yan , She Rui , Wu Jian Hua , Liu Shu Mei , Zhang Ming Zhu . Effect of Yunnan Baiyao on the Secretion of TNF-α, IL-6, IL-1β from Human Dental Pulp Cells under LPS-PG induced Inflammatory Environment[J]. Journal of Kunming Medical University, 2017, 38(08): 73-78.

Citation:

Huang Song Jing , Lei Ya Yan , She Rui , Wu Jian Hua , Liu Shu Mei , Zhang Ming Zhu . Effect of Yunnan Baiyao on the Secretion of TNF-α, IL-6, IL-1β from Human Dental Pulp Cells under LPS-PG induced Inflammatory Environment[J]. Journal of Kunming Medical University, 2017, 38(08): 73-78.

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Effect of Yunnan Baiyao on the Secretion of TNF-α, IL-6, IL-1β from Human Dental Pulp Cells under LPS-PG induced Inflammatory Environment

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基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2013FB178, 2014FZ039);

Received Date: 2017-04-10

Abstract

Abstract

Objective To investigate the effect of Yunnan Baiyao on the secretion of TNF-α, IL-6, IL-1βfrom human dental pulp cells ( h DPCs) under lipopolysaccharide from Porphyromonas gingivalis (LPS-PG) induced inflammatory environment, and investigate the inhibition effect of Yunnan Baiyao on pulpitis. Methods HDPCs were cultured by the modified tissue explant culture technique. Cultured h DPCs were identified and verified by the immunocytochemical staining. HDPCs were exposed to 10 μg/m L LPS-PG solution for 1 day, then the LPS-PG containing culture media were removed and collected, the h DPCs were divided into3 groups cultured with different new culture media, the control group was cultured with 1% FBS MEM, the LPS-PG group was cultured with 10 μg/m L LPS-PG, the Baiyao group was cultured with 10μg/m L Yunnan Baiyao. Culturing media were collected on exposure finishing, 1st and 3rd day after the medium changing for detection of IL-6, IL-1β and TNF-αproteins by ELISA, and on 3rd day the quantitative Real-Time PCR (q RT-PCR) was performed for IL-6, IL-1β and TNF-α gene expression of the h DPCs.Results HDPCs culturing showed a positive vimentin expression and a negative keratin expression. 3 groups showed no difference in TNF-α, IL-6 and IL-1β secretions immediately after the LPS-PG exposure for 1 day by ELISA, and the LPS-PG group showed higher secretions of TNF-α, IL-6 and IL-1β than other groups on1 st and 3rd day (P<0.05) . Baiyao group and control group showed no differences (P>0.05) . However, q RT-PCR showed lower gene expressions of TNF-α, IL-6, IL-1β in the Baiyao group than other groups at 3rd day (P <0.05) .Conclusion Under inflammatory environment induced by LPS-PG, the Yunnan Baiyao shows no inhibitory effect on secretion of TNF-α, IL-6 and IL-1β on3 rd day after the medium changing, but can downregulate the m RNA expression.
- Porphyromonas gingivali Lipopolysaccharide,
- Human dental pulp cells,
- TNF-α,
- IL-6,
- IL-1β

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References(26)

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