The Effects of Different Fluorescent Antibody Dosages of HLA-B27 on the Results of Flow Cytometry

Zhang Wei; Yang Guo Ji; Chen Wei Wen; Li Hong Wei; Li Xiu Ping; Zeng Xiao Yan

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Zhang Wei , Yang Guo Ji , Chen Wei Wen , Li Hong Wei , Li Xiu Ping , Zeng Xiao Yan . The Effects of Different Fluorescent Antibody Dosages of HLA-B27 on the Results of Flow Cytometry[J]. Journal of Kunming Medical University, 2017, 38(08): 98-101.

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Zhang Wei , Yang Guo Ji , Chen Wei Wen , Li Hong Wei , Li Xiu Ping , Zeng Xiao Yan . The Effects of Different Fluorescent Antibody Dosages of HLA-B27 on the Results of Flow Cytometry[J]. Journal of Kunming Medical University, 2017, 38(08): 98-101.

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The Effects of Different Fluorescent Antibody Dosages of HLA-B27 on the Results of Flow Cytometry

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基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2014FZ060);

Received Date: 2017-04-22

Abstract

Abstract

Objective To study the effects of different fluorescent antibody dosages of HLA-B27 on the flow cytometry results in peripheral blood lymphocyte of ankylosing spondylitis (AS) .Methods The expression intensities of peripheral blood lymphocyte HLA-B27 were detected in 22 cases of AS and 30 cases of healthy individual by the flow cytometry. The anti-human FITC-HLA-B27/PE-CD3 antibody kit was divided into five groups (2 μL, 4 μL, 8 μL, 15 μL, 30 μL) . BD company set the value of fluorescence intensity≥146 as a positive result. Results The expression intensity in the healthy individuals were (55.97 ±6.44) , (59.90 ±7.15) , (67.97±7.62) , (81.60±6.63) , (91.97±5.77) , while in AS were (122.45±7.38) , (140.50±5.35) , (148.22±4.36) , (162.27±4.28) , (164.05±4.28) . The results rose along with the dosages of the antibody, the results in the healthy were negative. But in AS, if the dosages of antibody were more than 8 μL, the results were positive, and the results were similar in 15 μL and 30 μL, while if the dosages of antibody were less than 8 μL, the results were false negative (P <0.05) . Conclusion The 8μL dosage of antibody was helpful to decrease the miss diagnosis of AS.Simultaneously, with the increase of the amount of antibodies, the results do not have a substantial increase.
- Ankylosing spondylitis,
- HLA-B27,
- Antibody dosage,
- Flow cytometry

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References(21)

References

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