Changes of Corneal Endothelial Cells after DCD Corneal Transplantation

Objective To study the variation of corneal endothelial cellsafterpenetrating keratoplasty from donation after cardiac death (DCD) .Methods 191 cases of corneal graftwere textedafter penetrating keratoplasty1-4 weeks; 5-12 weeks; 4-6 months; 7-12 months later from donation after cardiac death with corneal endothelial microscope.Results 1.48 patients in 191 cases of patients with corneal endothelial microscopeweredetected, 143 cases of patients could not be detected, the detection rate was 25%;2.48 cases after1-4 weeks, 5-12 weeks, 4-6 monthsand 7-12 months the density of endothelial cells were (2271.15±321.47) mm2, (1971.33±358.18) mm2, (1 826.59±303.92) mm2, (1753.14±306.31) mm2, and the average cell area increased from (388.45 ±95.26) μm to (638.63 ±124.73) μm 7-12 months after operation, cell size variation coefficient (CV value) increased from 30.15% to 65.04%, the proportion of hexagonal cells decreased from 52.59 ±7.26% to 40.01 ±11.35%.Conclusion (1) corneal endothelial endoscopy for early corneal endothelial cells of patients with recognition rate is low, poor sensitivity, confocal microscopy evaluation could be used instead of corneal endothelial microsco when the cornea was edema; (2) Corneal endothelial cell density decreased gradually and the percentage of hexagonal cells was descend and CV value were increased after penetratingkeratoplasty; (3) Follow-up should be strengthened after penetrating keratoplasty from donation after cardiac death (DCD) 1 a later especially three months later, when there were signs of early rejection, timely anti rejection treatment was very important for reducing early rejection.