Analysis of X/Y Sperm Concentration after Multistage Gradient Centrifugation

Luo Xi; Li Yong Gang; Li Ming Ying; Zeng Peng; Yan Jia Cong

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Luo Xi , Li Yong Gang , Li Ming Ying , Zeng Peng , Yan Jia Cong . Analysis of X/Y Sperm Concentration after Multistage Gradient Centrifugation[J]. Journal of Kunming Medical University, 2017, 38(09): 15-18.

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Luo Xi , Li Yong Gang , Li Ming Ying , Zeng Peng , Yan Jia Cong . Analysis of X/Y Sperm Concentration after Multistage Gradient Centrifugation[J]. Journal of Kunming Medical University, 2017, 38(09): 15-18.

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Analysis of X/Y Sperm Concentration after Multistage Gradient Centrifugation

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基金：云南省卫生科技计划基金资助项目 (2012WS0073);

Received Date: 2017-03-16

Abstract

Abstract

Objective In assisted reproduction, the X/Y sperm was sorted by multi density gradient centrifugation to examine whether the proportion of X/Y sperm was unbalanced in conventional IVF-ET.Method The use of Sperm Grad (Vitrolife, Sweden) and Sperm Rinse (Vitrolife, Sweden) to prepare 10 gradient liquid layer, were 100%, 95%, 90%, 85%, 80%, 75%, 70%, 65%, 60%, 55%.After the multilayer gradient was made in the centrifuge tube, the boundary between each gradient was obvious.Choose liquefaction normal, concentration of about 50×106/mL, activity rate of about 50%, low deformity rate of 5 people, after mixing semen, removing individual differences, and take 3 mL mixture gradient centrifugation.Collect each gradient bottom sperm, using two-color X/Y FISH probe (Vysis) for detection, and statistics X/Y sperm percentage.Results 100%, 95%, 90%, 85%, 80%, The proportion of X/Y sperm in each gradient was: 51.41%, 51.41%, 52.19%, 50.99%, 54.10%.Conclusions Although the proportion of X/Y sperm in each gradient was different, the difference was not obvious.In assisted reproductive technology, the use of commercialized sperm washing fluid, even if the use of multi-layer gradient centrifugation, will not cause gender imbalance.
- Gradient centrifugation,
- X/Y sperm sorting,
- FISH,
- Fluorescence in situ hybridization

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References(16)

References

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