Comparison of Proliferation between Fetal Forebrain-and Spinal Cord-Derived Neural Stem Cells in Mice

Zhao Yu; Yuan Jing; Liu Jian Jun; Lu Lin

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Zhao Yu , Yuan Jing , Liu Jian Jun , Lu Lin . Comparison of Proliferation between Fetal Forebrain-and Spinal Cord-Derived Neural Stem Cells in Mice[J]. Journal of Kunming Medical University, 2017, 38(09): 19-23.

Citation:

Zhao Yu , Yuan Jing , Liu Jian Jun , Lu Lin . Comparison of Proliferation between Fetal Forebrain-and Spinal Cord-Derived Neural Stem Cells in Mice[J]. Journal of Kunming Medical University, 2017, 38(09): 19-23.

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Comparison of Proliferation between Fetal Forebrain-and Spinal Cord-Derived Neural Stem Cells in Mice

Zhao Yu ¹,
Yuan Jing ²,
Liu Jian Jun ²,
Lu Lin ^2,3

Funds:

基金：云南省应用基础研究基金资助项目 (2014C039Y);

Received Date: 2017-04-19

Abstract

Abstract

Objective To discusses the differences of proliferation between fetal forebrain-and spinal cord-derived neural stem cells (NSCs) in mice.Methods The NSCs were separated by the mechanical method from the forebrain and spinal cord of 14 day C57 BL foetus, and the immunocyfluorescent staining of Nestin & Sox2 used to as the NSCs markers.Meanwhile, calculating multiplication rate and cell doubling time, with the addition of Brd U/Nestin double staining were used to test the proliferation.Results Firstly, multiplication rate of forebrain-derived NSCs significantly proliferated faster than spinal cord-derived (P<0.05) , and cell doubling time is inversely proportional to NSCs multiplication.Secondly, the 5 passages of these two sources of NSCs were the fastest.Lastly, the Brd U+ NSCs also suggested that the proliferating forebrain-derived NSCs (29.65%±3.38) were significantly more than spinal cord-derived (29.65%±3.38) (P<0.05) .Conclusion Our study suggests that the forebrain-derived NSCs have more capacity of proliferation.
- Neural stem cells,
- Proliferation,
- Forebrain,
- Spinal cord

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References(12)

References

[1]	[1]GAGE FH.Mammalian neural stem cells[J].Scicnce, 2000, 287 (5457) :1433-1438.[1]GAGE FH.Mammalian neural stem cells[J].Scicnce, 2000, 287 (5457) :1433-1438.
[2]	[2]REYNOLDS BA, WEISS S.Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system[J].Science, 1992, 255 (5052) :1707-1710.[2]REYNOLDS BA, WEISS S.Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system[J].Science, 1992, 255 (5052) :1707-1710.
[3]	[3]WEISS S, DUNNE C, HEWSON J, et al.Multipotent CNS stem cells are present in the adult mammalian spinal cord and ventricular neuroaxis[J].J Neurosci, 1996, 16 (23) :7599-7609.[3]WEISS S, DUNNE C, HEWSON J, et al.Multipotent CNS stem cells are present in the adult mammalian spinal cord and ventricular neuroaxis[J].J Neurosci, 1996, 16 (23) :7599-7609.
[4]	[4]DAVIS AA, TEMPLE S.A self-renewing multipotential stem cell in embryonic rat cerebral cortex[J].Nature, 1994, 372 (6503) :263-266.[4]DAVIS AA, TEMPLE S.A self-renewing multipotential stem cell in embryonic rat cerebral cortex[J].Nature, 1994, 372 (6503) :263-266.
[5]	[5]JOHANSSON C B, MOMMA S, CLARKE D L, et al.Identification of a neural stem cell in the adult mammalian central nervous system[J].Cell, 1999.96 (1) :25-34.[5]JOHANSSON C B, MOMMA S, CLARKE D L, et al.Identification of a neural stem cell in the adult mammalian central nervous system[J].Cell, 1999.96 (1) :25-34.
[6]	[6]KOMBLUM HI.Introduction to neural stem cells[J].Stroke;a journal of cerebral circulation, 2007, 38 (2) :810-816.[6]KOMBLUM HI.Introduction to neural stem cells[J].Stroke;a journal of cerebral circulation, 2007, 38 (2) :810-816.
[7]	[7]KANEKO Y, SAKAKIBARA S, IMAI T, et al.An evolutionally conserved marker for CNS progenitor cells including neural stem cells[J].Developmental neuroscience2000, 22 (1-2) :139-153.[7]KANEKO Y, SAKAKIBARA S, IMAI T, et al.An evolutionally conserved marker for CNS progenitor cells including neural stem cells[J].Developmental neuroscience2000, 22 (1-2) :139-153.
[8]	[8]ENGLUND U, FRICKER-GATES RA, LUNDBERG C, et al.Transplantation of human neural progenitor cells into the neonatal rat brain:extensive migration and differentiation with long-distance axonal projections[J].Experimental neurology, 2002, 173 (1) :1-21.[8]ENGLUND U, FRICKER-GATES RA, LUNDBERG C, et al.Transplantation of human neural progenitor cells into the neonatal rat brain:extensive migration and differentiation with long-distance axonal projections[J].Experimental neurology, 2002, 173 (1) :1-21.
[9]	[9]MCKAY R.Stem cells in the central nervous system[J].Science, 1997, 276 (5309) :66-7l.[9]MCKAY R.Stem cells in the central nervous system[J].Science, 1997, 276 (5309) :66-7l.
[10]	[10]BRUSTLE O, JONES K N, LEARISH R D, et al.Embryonic stem cell-derived glial precursors:A sourse of myelinating transplants[J].Science, 1999, 285 (5428) :754-756.[10]BRUSTLE O, JONES K N, LEARISH R D, et al.Embryonic stem cell-derived glial precursors:A sourse of myelinating transplants[J].Science, 1999, 285 (5428) :754-756.
[11]	[11]PAGANO S F, IMPAGNATIELLO F, GIRELLI M, et al.Isolation and characterization of neural stem cells from the adult human olfactory bulb[J].Stem Cells, 2000, 18 (4) :295-300.[11]PAGANO S F, IMPAGNATIELLO F, GIRELLI M, et al.Isolation and characterization of neural stem cells from the adult human olfactory bulb[J].Stem Cells, 2000, 18 (4) :295-300.
[12]	[12]WATANABE K, NAKAMURA M, IWANAMI A, et al.Comparison between fetal spinal-cord-and forebrain-derived neural stem/progenitor cells as a source of transplantation for spinal crd injury[J].Dev Neurosci, 2004, 26 (2-4) :275-287.[12]WATANABE K, NAKAMURA M, IWANAMI A, et al.Comparison between fetal spinal-cord-and forebrain-derived neural stem/progenitor cells as a source of transplantation for spinal crd injury[J].Dev Neurosci, 2004, 26 (2-4) :275-287.

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