Cleaning Efficacy of Different Pretreatment Methods on Surgical Instruments Contaminated with Hepatitis B Virus

Li Yan Qiong; Yang Chun Lian; Lin Xiao Ming; Sa Ya Lian; Sun 鷖; Yuan Qin

Volume 38 Issue 10

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Li Yan Qiong , Yang Chun Lian , Lin Xiao Ming , Sa Ya Lian , Sun 鷖, Yuan Qin . Cleaning Efficacy of Different Pretreatment Methods on Surgical Instruments Contaminated with Hepatitis B Virus[J]. Journal of Kunming Medical University, 2017, 38(10): 120-123.

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Cleaning Efficacy of Different Pretreatment Methods on Surgical Instruments Contaminated with Hepatitis B Virus

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基金：云南省教育厅科研基金资助项目 (2015C005Y);

Received Date: 2017-04-23

Abstract

Abstract

Objective To analyze the influence of different pretreated methods on cleaning efficacy of surgical instruments polluted by hepatitis B virus (HBV) .Methods 600 HBV-contaminated surgical instruments were selected and divided randomly into 4 groups of pretreatment methods A, B, C and D, after pretreatment, four groups of instruments were washed in the verified automatic cleaning sterilizer. After washing, instruments were washed with pure water, the samples were taken, and the cleanliness of the instruments was inspected visually and by 5-time magnifier that has light source; the cleanliness was test by 3 M ATP fluorescence method; the positive rate of HBs Ag was detected by Quantitative ELISA.Results The visual pass rate of the cleanliness of 600 HBV-contaminated surgical instruments after four types of pretreatment was 100.00%, 82.67%, 100.00% and 90.67% respectively; which was 100.00%, 78.67%, 97.33% and 85.33% by 5-time magnifier that has light source; which was 98.67%, 72.67%, 96.00% and 80.00% by 3 M ATP fluorescence method;With regard to the above three test results, Group A was compared with Group B、Group D and Group B was compared with Group C while the differences were statistically significant (P<0.01) .The positive rate of HBs Ag detected by ELISA was 0. Conclusion Multiple Enzyme Detergent solution (1:270) can significantly improve the cleaning quality of HBV-contaminated surgical instruments, and reduce instruments loss.
- Hepatitis B Virus,
- Surgical instruments,
- Pretreatment,
- Cleaning and disinfection,
- Multiple Enzyme Detergent,
- Cleaning effect

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