Identification of Radix Saussureae lappae from Different Habitats by HPLC Fingerprints Combined with Chemometrics

Lu Min Nan; Yang Xue Fang; Deng Ju Qing; Hu Yue Xin; Li Fan; Wu Lin Xiong; Kong Can; Wu Yi

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Article Navigation > Journal of Kunming Medical University > 2017 > 38(10): 16-21

Lu Min Nan , Yang Xue Fang , Deng Ju Qing , Hu Yue Xin , Li Fan, Wu Lin Xiong , Kong Can , Wu Yi . Identification of Radix Saussureae lappae from Different Habitats by HPLC Fingerprints Combined with Chemometrics[J]. Journal of Kunming Medical University, 2017, 38(10): 16-21.

Citation:

Lu Min Nan , Yang Xue Fang , Deng Ju Qing , Hu Yue Xin , Li Fan, Wu Lin Xiong , Kong Can , Wu Yi . Identification of Radix Saussureae lappae from Different Habitats by HPLC Fingerprints Combined with Chemometrics[J]. Journal of Kunming Medical University, 2017, 38(10): 16-21.

Citation:

Lu Min Nan , Yang Xue Fang , Deng Ju Qing , Hu Yue Xin , Li Fan, Wu Lin Xiong , Kong Can , Wu Yi . Identification of Radix Saussureae lappae from Different Habitats by HPLC Fingerprints Combined with Chemometrics[J]. Journal of Kunming Medical University, 2017, 38(10): 16-21.

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Identification of Radix Saussureae lappae from Different Habitats by HPLC Fingerprints Combined with Chemometrics

Funds:

基金：云南省教育厅科学研究基金资助项目 (2013C246); 昆明医科大学教研教改课题 (2017-JY-Y-004);

Received Date: 2017-05-06

Abstract

Abstract

Objectives To identify Radix Saussureae lappae from different habitats by application of HPLC fingerprints and chemometrics. Methods By using reversed phase high performance liquid chromatography (HPLC) , the analysis was carried out with the mobile phases of Acetonitrile and water by gradient elution. The flow rate was 1.0 m L/min, and the UV detection wave length was 254 nm. Similarity calculation, principal component analysis and hierarchical cluster analysis were applied to read HPLC fingerprints, then to identify of Radix Saussureae lappae from different habitats. Results The method could be used to separate the components of Radix Saussureae lappae. According to the test results, 18 common fingerprint peaks were assigned, and a common HPLC fingerprint pattern of Radix Saussureae lappae was established, and the similarity of Radix Saussureae lappae from 15 different sources was high. But there were significant differences in the fingerprint patterns of samples from different habitats. Conclusion With excellent specificity and reproducibility, the fingerprinting method provides a feasible method for identification of Radix Saussureae lappae from different habitats, and can provide references for Radix Saussureae lappae quality control, and provide references for resources reasonable development and utilization of Radix Saussureae lappae.
- Radix Saussureae lappae,
- HPLC,
- Fingerprints,
- Chemometrics

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References(19)

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