Small Interfering RNA Silencing VDR in HK-2 Cells and Its Significance for Hypocitraturia

Li Kang Jian; Luo Yu Hui; Mo Yin; Shen Ji Hong; Liu Xiao Dong; Li Hao

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Li Kang Jian , Luo Yu Hui , Mo Yin , Shen Ji Hong , Liu Xiao Dong , Li Hao . Small Interfering RNA Silencing VDR in HK-2 Cells and Its Significance for Hypocitraturia[J]. Journal of Kunming Medical University, 2017, 38(10): 22-26.

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Li Kang Jian , Luo Yu Hui , Mo Yin , Shen Ji Hong , Liu Xiao Dong , Li Hao . Small Interfering RNA Silencing VDR in HK-2 Cells and Its Significance for Hypocitraturia[J]. Journal of Kunming Medical University, 2017, 38(10): 22-26.

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Small Interfering RNA Silencing VDR in HK-2 Cells and Its Significance for Hypocitraturia

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基金：国家自然科学基金资助项目 (81460141);

Received Date: 2017-04-02

Abstract

Abstract

Objective To screen small interfering ribonucleic acid (si RNA) that can effectively silence the vitamin D receptor (VDR) of human renal proximal tubular epithelial cells (HK-2 cells) and its significance for hypocitraturia. Methods We cultured HK-2 cells, designed 4 VDR-si RNA, used packaged lentivirus to infect HK-2 cells and detected the expression level of VDR protein and m RNA of HK-2 cells whose VDR gene silenced by Western blot and real-time quantitative PCR. Results Si RNA 1 (6120) could significantly inhibit the expression of VDR in HK-2 cells, and si RNA VDR-1 (6120) stable cell lines were obtained. Conclusion VDR-si RNA 1 (6120) can effectively silence the expression of VDR in HK-2 cells, and we can detect the expression of Na DC1 after VDR expression is inhibited in the next step.
- HK-2 cells,
- Vitamin D receptor,
- Small interfering ribonucleic acid,
- Hypocitraturia

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References(14)

References

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