Li Kang Jian , Luo Yu Hui , Mo Yin , Shen Ji Hong , Liu Xiao Dong , Li Hao . Small Interfering RNA Silencing VDR in HK-2 Cells and Its Significance for Hypocitraturia[J]. Journal of Kunming Medical University, 2017, 38(10): 22-26.
Citation: Li Kang Jian , Luo Yu Hui , Mo Yin , Shen Ji Hong , Liu Xiao Dong , Li Hao . Small Interfering RNA Silencing VDR in HK-2 Cells and Its Significance for Hypocitraturia[J]. Journal of Kunming Medical University, 2017, 38(10): 22-26.

Small Interfering RNA Silencing VDR in HK-2 Cells and Its Significance for Hypocitraturia

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基金: 国家自然科学基金资助项目 (81460141);

  • Received Date: 2017-04-02
  • Objective To screen small interfering ribonucleic acid (si RNA) that can effectively silence the vitamin D receptor (VDR) of human renal proximal tubular epithelial cells (HK-2 cells) and its significance for hypocitraturia. Methods We cultured HK-2 cells, designed 4 VDR-si RNA, used packaged lentivirus to infect HK-2 cells and detected the expression level of VDR protein and m RNA of HK-2 cells whose VDR gene silenced by Western blot and real-time quantitative PCR. Results Si RNA 1 (6120) could significantly inhibit the expression of VDR in HK-2 cells, and si RNA VDR-1 (6120) stable cell lines were obtained. Conclusion VDR-si RNA 1 (6120) can effectively silence the expression of VDR in HK-2 cells, and we can detect the expression of Na DC1 after VDR expression is inhibited in the next step.
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  • [1]
    [1]SONG Y, HERNANDEZ N, SHOAG J, et al.Potassium citrate decreases urine calcium excretion in patients with hypocitraturic calcium oxalate nephrolithiasis[J].Urolithiasis, 2016, 44 (2) :145-148.
    [2]李康健, 莫茵, 申吉泓, 等.维生素D受体基因SNP位点rs7975232和rs731236与云南彝族人群特发性低枸橼酸尿症的关系[J].广东医学, 2016, 37 (17) :2580-2584.
    [3]李康健, 莫茵, 申吉泓, 等.VDR和NaD C1在HK-2细胞中的表达及其对低枸橼酸尿症的意义[J].昆明医科大学学报, 2016, 37 (1) :35-39.
    [4]
    [4]TURNEY B, ROBERTSON W, WISEMAN O, et al.Use of the probability of stone formation (PSF) score to assess stoneforming risk and treatment response in a cohort of Brazilian stone formers[J].Int Braz J Urol, 2014, 40 (4) :507-512.
    [5]
    [5]JHAGROO R A, WERTHEIM M L, PENNISTON K L.Alkali replacement raises urinary citrate excretion in patients with topiramate-induced hypocitraturia[J].Br J Clin Pharmacol, 2016, 81 (1) :131-136.
    [6]
    [6]AKDENIZ A, CAGLAYAN M G, ANZENBACHER P J R.A tri-serine tri-lactone scaffold for the quantification of citrate in urine[J].Chem Commun (Camb) , 2016, 52 (9) :1827-1830.
    [7]
    [7]OHANA E, SHCHEYNIKOV N, MOE O W, et al.Slc26a6and nadc-1 transporters interact to regulate oxalate and citrate homeostasis[J].J Am Soc Nephrol, 2013, 24 (10) :1617-1626.
    [8]
    [8]RENDINA D, DE FILIPPO G, GIANFRANCESCO F, et al.Evidence for epistatic interaction between VDR and SLC13A2 genes in the pathogenesis of hypocitraturia in recurrent calcium oxalate stone formers[J].J Nephrol, 2017, 30 (3) :411-418.
    [9]
    [9]BOS S, NAP R R, WOUTERS R S, et al.Hypocitraturia:a common but not well known cause of nephrolithiasis[J].Neth J Med, 2014, 72 (10) :545-547.
    [10]
    [10]HALIGA R, ZUGUN-ELOAE F, OBOROCEANU T, et al.Vitamin D and tissular expression of vitamin D receptor in obesity[J].Rev Med Chir Soc Med Nat Iasi, 2016, 120 (2) :404-408.
    [11]
    [11]AL-DAGHRI N M, AL-ATTAS O S, ALKHARFY K M, et al.Association of VDR-gene variants with factors related to the metabolic syndrome, type 2 diabetes and vitamin D deficiency[J].Gene, 2014, 542 (2) :129-133.
    [12]
    [12]MAO S, HUANG S.Vitamin D receptor gene polymorphisms and the risk of rickets among Asians:a meta-analysis[J].Arch Dis Child, 2014, 99 (3) :232-238.
    [13]
    [13]NG A H, FRICK K K, KRIEGER N S, et al.1, 25 (OH) (2) D (3) induces a mineralization defect and loss of bone mineral density in genetic hypercalciuric stone-forming rats[J].Calcif Tissue Int, 2014, 94 (5) :531-543.
    [14]
    [14]LIN W, YAN W, YANG W, et al.Characterisation of siRNAs derived from new isolates of bamboo mosaic virus and their associated satellites in infected ma bamboo Dendrocalamus latiflorus[J].Arch Virol, 2017, 162 (2) :505-510.
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