Effects of Methamphetamine on the Expression of DA, 5-HT and TNF-α in Rats Hippocampus

Yang Jin Feng; Meng Guo Yi; Sui Nian Han; Bian Li Gong; Hong Shi Jun; Li Li Hua; Zhao Yong Na

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Yang Jin Feng , Meng Guo Yi , Sui Nian Han , Bian Li Gong , Hong Shi Jun , Li Li Hua , Zhao Yong Na . Effects of Methamphetamine on the Expression of DA, 5-HT and TNF-α in Rats Hippocampus[J]. Journal of Kunming Medical University, 2017, 38(10): 6-10.

Citation:

Yang Jin Feng , Meng Guo Yi , Sui Nian Han , Bian Li Gong , Hong Shi Jun , Li Li Hua , Zhao Yong Na . Effects of Methamphetamine on the Expression of DA, 5-HT and TNF-α in Rats Hippocampus[J]. Journal of Kunming Medical University, 2017, 38(10): 6-10.

Citation:

Yang Jin Feng , Meng Guo Yi , Sui Nian Han , Bian Li Gong , Hong Shi Jun , Li Li Hua , Zhao Yong Na . Effects of Methamphetamine on the Expression of DA, 5-HT and TNF-α in Rats Hippocampus[J]. Journal of Kunming Medical University, 2017, 38(10): 6-10.

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Effects of Methamphetamine on the Expression of DA, 5-HT and TNF-α in Rats Hippocampus

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基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2014FB018);

Received Date: 2017-04-21

Abstract

Abstract

Objective To study the effect of methamphetamine on the expression of DA, 5-HTand TNF-αin rats hippocampus. Methods (1) The in vivo toxicity model was established by injecting MA 15 mg/ (kg.time) , two times a day for 5 days. The in vitro toxicity model was established by incubating MA with mixed hippocampal neurons/microglia for 24 hours. (2) ELISA was used to detect the content of DA, 5-HT and TNF-α in rat hippocampus and cell culture supernatant. Results On the fifth day, Methamphetamine treated rats showed hair errecting, weight loss, rapid head shaking, circling or head dorsal movement, with a stereotyped score of 5. The levels of DA and 5-HT decreased, and the content of TNF-alpha elevated in rats hippocampus of MA group. The levels of DA, 5-HT decreased and the content of TNF-alpha increased in cell cultural supernatants of MA group24 hours. Conclusion The changes of DA, 5-HT and TNF-alpha are involved in rat hippocampal damage induced by methamphetamine.
- Methamphetamine,
- Hippocampus,
- Mixed hippocampal neurons/microglia,
- DA,
- 5-HT,
- TNF-α

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References(20)

References

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