Infection of Pseudomonas aeruginosa Activates TGF-β1 Smad Signaling Pathway in Human Middle Ear Epithelial Cells

Long Rui Qing; Liu Zhuo Hui; Zhang Fan; Li Shu Ling; Yu Yong Mei; Ruan Biao

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Article Navigation > Journal of Kunming Medical University > 2017 > 38(10): 65-69

Long Rui Qing , Liu Zhuo Hui , Zhang Fan , Li Shu Ling , Yu Yong Mei , Ruan Biao . Infection of Pseudomonas aeruginosa Activates TGF-β1 Smad Signaling Pathway in Human Middle Ear Epithelial Cells[J]. Journal of Kunming Medical University, 2017, 38(10): 65-69.

Citation:

Long Rui Qing , Liu Zhuo Hui , Zhang Fan , Li Shu Ling , Yu Yong Mei , Ruan Biao . Infection of Pseudomonas aeruginosa Activates TGF-β1 Smad Signaling Pathway in Human Middle Ear Epithelial Cells[J]. Journal of Kunming Medical University, 2017, 38(10): 65-69.

Citation:

Long Rui Qing , Liu Zhuo Hui , Zhang Fan , Li Shu Ling , Yu Yong Mei , Ruan Biao . Infection of Pseudomonas aeruginosa Activates TGF-β1 Smad Signaling Pathway in Human Middle Ear Epithelial Cells[J]. Journal of Kunming Medical University, 2017, 38(10): 65-69.

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Infection of Pseudomonas aeruginosa Activates TGF-β1 Smad Signaling Pathway in Human Middle Ear Epithelial Cells

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基金：国家自然科学基金资助项目 (81660175); 云南省卫生科技计划基金资助项目 (2014NS193);

Received Date: 2017-04-22

Abstract

Abstract

Objective To study the molecular mechanism of the infection process of Pseudomonas aeruginosa in human middle ear epithelial cells (HMEECs) . Methods Real-time PCR and Western blotting method were used to detect the m RNA and protein levels of TGF-β1 and Smad2, and the phosphorylation of Smad7 in the infection process of Pseudomonas aeruginosaonhuman middle ear epithelial cells. Results Infection of Pseudomonas aeruginosa in human middle ear epithelial cells significantly increased the m RNA and protein levels of TGF-β1 and Smad2, and also inhibited the phosphorylation of Smad7. Mutation of Opr F and PKC inhibitor Bisindolylmaleimide I could derease the m RNA and protein levels of TGF-β1 and Smad2 and the enhanced the phosphorylation of Smad7 compared with Pseudomonasaeruginosa infected HMEECs. Conclusion Infection of Pseudomonas aeruginosaonhuman activates the TGF-β1 Smad signaling pathway in middle ear epithelial cells.
- Pseudomonas aeruginosa,
- Human middle ear epithelial cells,
- TGF-β1 Smadsignalingpathway,
- PKC

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References(14)

References

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