Overexpression of miR-4328 Inhibits the Proliferation, Migration and Epithelial Mesenchymal Transition of Prostate Cancer Cells

Zhao Bin; Li Rui Qian; Zhang Guo Ying; Lei Yong Hong; Wang Qi Lin; Liu Zhi Min

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Article Navigation > Journal of Kunming Medical University > 2017 > 38(11): 29-33

Zhao Bin , Li Rui Qian , Zhang Guo Ying , Lei Yong Hong , Wang Qi Lin , Liu Zhi Min . Overexpression of miR-4328 Inhibits the Proliferation, Migration and Epithelial Mesenchymal Transition of Prostate Cancer Cells[J]. Journal of Kunming Medical University, 2017, 38(11): 29-33.

Citation:

Zhao Bin , Li Rui Qian , Zhang Guo Ying , Lei Yong Hong , Wang Qi Lin , Liu Zhi Min . Overexpression of miR-4328 Inhibits the Proliferation, Migration and Epithelial Mesenchymal Transition of Prostate Cancer Cells[J]. Journal of Kunming Medical University, 2017, 38(11): 29-33.

Citation:

Zhao Bin , Li Rui Qian , Zhang Guo Ying , Lei Yong Hong , Wang Qi Lin , Liu Zhi Min . Overexpression of miR-4328 Inhibits the Proliferation, Migration and Epithelial Mesenchymal Transition of Prostate Cancer Cells[J]. Journal of Kunming Medical University, 2017, 38(11): 29-33.

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Overexpression of miR-4328 Inhibits the Proliferation, Migration and Epithelial Mesenchymal Transition of Prostate Cancer Cells

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基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2014FB068);

Received Date: 2017-06-12

Abstract

Abstract

Objective To study the role and molecular mechanism of miR-4328 in prostate cancer.Methods CCK-8 method was used to detect the proliferation of prostate cancer cells, and Transwell method was used to detect the migration of prostate cancer cells, and Real-time PCR and Western blotting method were used to detect the epithelial mesenchymal transition markers and the expression of ZEB1.Results The expression level of miR-4328 in prostate cancer tissues was significantly lower than that in paracancerous tissues.Overexpression of miR-4328 in prostate cancer cells significantly inhibited the cell proliferation (P<0.001) .And overexpression of miR-4328 also significantly inhibited the migration of prostate cancer cells (P<0.001) .And overexpression of miR-4328 up-regulated the epithelial cell marker E-cadherin, and down-regulated the mesenchymal cell markers including N-cadherin and Vimentin (P <0.001) transition of prostate cancer cells.Further studies found that overexpression of miR-4328 could down regulate the expression level of epithelial mesenchymal transition related transcription factor ZEB1 (P<0.001) .Conclusion Overexpression of miR-4328 can inhibit the proliferation, migration and epithelial mesenchymal transition of prostate cancer cells.
- miR-4328,
- Prostate cancer,
- Epithelial mesenchymal transition,
- ZEB1

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References(14)

References

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