HBx Suppresses miR-338 Expression in Hepatoma via DNA Hypermethylation

Wu Gang

Volume 38 Issue 11

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Wu Gang . HBx Suppresses miR-338 Expression in Hepatoma via DNA Hypermethylation[J]. Journal of Kunming Medical University, 2017, 38(11): 5-8.

Citation:

Wu Gang . HBx Suppresses miR-338 Expression in Hepatoma via DNA Hypermethylation[J]. Journal of Kunming Medical University, 2017, 38(11): 5-8.

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HBx Suppresses miR-338 Expression in Hepatoma via DNA Hypermethylation

Wu Gang

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基金：国家自然科学基金资助项目 (81302161); 四川省卫计委科研基金资助项目 (150215);

Received Date: 2017-05-11

Abstract

Abstract

Objective To explore the intereaction between hypermethylation and non-coding RNAs related to Hepatitis B virus X protein (HBx) .hypermethylation.Methods miRNA microarray and quantitative reverse-transcription polymerase chain reactions (qRT-PCRs) were performed to confirm that HBx suppressed miR-338 expression in HCC cells.Protein, mRNA, miRNA expression analyses and promotor methylation detections were performed to delineate the mechanisms of HBx/HBV associated miR-338 repression in HCC.Results HBx suppressed miR-338 expression in HCC tissues and cells, while upregulating expression of DNMT1 and DNMT3A.The promotor methlation status of miR-338 was significantly higher in HBV+HCC tumors than adjacent tissues, while demethylation treatment could rescue the miR-338 expression in HBx+HCC cells.Conclus ions HBx can downregulate the expression of miR-338, while upregulate DNMT 3 A and DNMT 1 expression.The HBx associated promotor hypermethylation of miR-338 is responsible for its silence.
- HBx,
- miR-338,
- Methylation,
- Hepatocellular carcinoma

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