The Detection and Analysis of the Placenta Microbiota of Normal Term Delivery

Xiong Jing Jing; Qi Wen Jin; Hu Hong Wei; Zeng Jie; Huang Rong; Ma Run Mei; Liu Mei; Huang Yong Kun

Volume 38 Issue 11

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Xiong Jing Jing , Qi Wen Jin , Hu Hong Wei , Zeng Jie , Huang Rong , Ma Run Mei , Liu Mei , Huang Yong Kun . The Detection and Analysis of the Placenta Microbiota of Normal Term Delivery[J]. Journal of Kunming Medical University, 2017, 38(11): 52-57.

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Xiong Jing Jing , Qi Wen Jin , Hu Hong Wei , Zeng Jie , Huang Rong , Ma Run Mei , Liu Mei , Huang Yong Kun . The Detection and Analysis of the Placenta Microbiota of Normal Term Delivery[J]. Journal of Kunming Medical University, 2017, 38(11): 52-57.

Citation:

Xiong Jing Jing , Qi Wen Jin , Hu Hong Wei , Zeng Jie , Huang Rong , Ma Run Mei , Liu Mei , Huang Yong Kun . The Detection and Analysis of the Placenta Microbiota of Normal Term Delivery[J]. Journal of Kunming Medical University, 2017, 38(11): 52-57.

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The Detection and Analysis of the Placenta Microbiota of Normal Term Delivery

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基金：国家自然科学基金资助项目 (81360068); “十二五”云南省特色学科群建设项目 (201009);

Received Date: 2017-05-21

Abstract

Abstract

Objective To study the composition of the placenta microbiota of normal term delivery.Method Three placenta specimens were collected from full-term infant birth by normal spontaneous delivery.The flora DNA was extracted by Tissue Kit for 16SrRNA gene high-throughput nucleotide sequencing, then the sequencing results were analyzed.Results The placenta microbiota of normal spontaneous delivery at the bacterial phylum level showed five bacterial phylum were detected, including Proteobacteria, Unclassified Bacteria, Actinobacteria, Firmicutes and Bacteroidetes.Proteobacteria was the main parts of bacteria and more than 90 percent in the placenta microbiota.At the genus level, 24 bacterial genus were detected including Proteobacteria, Actinobacteria and Firmicutes.Halomona, Pelagibacterium and Unclassified Phyllobacteriacea of Proteobacteria genus was the main bacterial genus in the placenta microbiota.Actinotalea, Escherichia/Shigella, Sphingomonas, Bacteroides, Streptococcus, Klebsiella, Bifidobacterium, Clostridium and Staphylococcus.Conclusion There are abundant bacterial flora colonized in the normal term placenta.
- Placenta,
- Microbiota,
- High-throughput nucleotide sequencing

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References(13)

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