Effects of Roux-en-Y Gastric Bypass on Intestinal Thickening Bacteria and APPL1 in Rats

Wu Xue Song; Sun Feng; Bao Tian Hao; Ke Yang; Wang Ying Hua; Wang Hua

Volume 38 Issue 11

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Zhao Xia . Clinical Effect of Laparoscopy Combined with Gestrinone in Treatment of Ovarian Endometriosis Cyst and the Prognosis of Patients[J]. Journal of Kunming Medical University, 2016, 37(06).

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Wu Xue Song , Sun Feng , Bao Tian Hao , Ke Yang , Wang Ying Hua , Wang Hua . Effects of Roux-en-Y Gastric Bypass on Intestinal Thickening Bacteria and APPL1 in Rats[J]. Journal of Kunming Medical University, 2017, 38(11): 9-14.

Zhao Xia . Clinical Effect of Laparoscopy Combined with Gestrinone in Treatment of Ovarian Endometriosis Cyst and the Prognosis of Patients[J]. Journal of Kunming Medical University, 2016, 37(06).

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Effects of Roux-en-Y Gastric Bypass on Intestinal Thickening Bacteria and APPL1 in Rats

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基金：国家自然科学基金资助项目 (81660399); 云南省应用基础研究计划项目 (201401UH00302);

Received Date: 2017-05-15

Abstract

Abstract

Objective To investigate the effect of Roux-en-Y gastric bypass on intestinal thick-walled bacteria and APPL1.Methods The effect of postoperative and postoperative fecal transplantation on the metabolic index of rats was observed by Roux-en-Y gastric bypass.The thick-walled bacteria and APPL1 expression were detected by Western blot and qPCR.Results The body weight of the rats in the S+T group was significantly higher than that in the S group (P<0.05) .The body weight of the rats in the S+T group was significantly higher than that in the control group (P<0.05) The expression of TG in S+T group was significantly higher than that in S group (P<0.05) .The expression of TG in S group was significantly lower than that in group C (P<0.05) The expression of TCH in the T group was significantly higher than that in the S group (P<0.05) .The expression of TCH in the S group was significantly higher than that in the S group (P<0.05) The expression of FINS in S group was significantly higher than that in group C (P<0.05) .The expression of FINS in S group was significantly higher than that in group S (P<0.05) .The expression of ISI in S group was significantly higher than that in group S (P<0.05) .The expression of ISI in S group was significantly higher than that in group S (P<0.05) .The expression of S thick-walled bacteria in S was significantly lower than that in group C (P <0.05) .Western blot and mRNA detection showed that the expression of APPL1 in S group was significantly higher than that in group C (P<0.01) .The expression of APPL1 in S+T group was significantly lower than that in S group (P <0.05) .Conclus ion Roux-en-Y gastric bypass may be mediated by affecting the expression of thick-walled flora.
- Roux-en-Y gastric bypass,
- Firmicutes,
- APPL1,
- Body weight

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