Thyroid Papillary Carcinoma Cells TPC-1 Apoptosis Induced by Ursolic Acid

Hua Jie; Ruan Ling Yu; Ma Ding Qian; Zuo Xiao Shuang; Xiang Run Qing; Fang Shan Dan; Fan Yuan

Volume 39 Issue 01

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Hua Jie , Ruan Ling Yu , Ma Ding Qian , Zuo Xiao Shuang , Xiang Run Qing , Fang Shan Dan , Fan Yuan . Thyroid Papillary Carcinoma Cells TPC-1 Apoptosis Induced by Ursolic Acid[J]. Journal of Kunming Medical University, 2018, 39(01): 10-15.

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Hua Jie , Ruan Ling Yu , Ma Ding Qian , Zuo Xiao Shuang , Xiang Run Qing , Fang Shan Dan , Fan Yuan . Thyroid Papillary Carcinoma Cells TPC-1 Apoptosis Induced by Ursolic Acid[J]. Journal of Kunming Medical University, 2018, 39(01): 10-15.

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Thyroid Papillary Carcinoma Cells TPC-1 Apoptosis Induced by Ursolic Acid

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基金：云南省应用基础研究计划项目-中医联合重点基金资助项目 (2017FF117 (-008) );

Received Date: 2017-07-11

Abstract

Abstract

Objective To study the inhibitory effect of ursolic acid on the proliferation of human papillary thyroid carcinoma cell line TPC-1 in vitro. Method TPC-1 cells were treated with different concentrations of ursolic acid (control group:0μM, experimental group:3μM, 6μM, 12μM) ; MTT assay was used to observe the effect of the growth of TPC-1 cells on different concentrations of ursolic acid at the same time; Apoptosis and cell cycle distribution of TPC-1 cells were treated with ursolic acid by flow cytometry; The expression of Bcl-2, Bax and Caspase-9 mRNA in TPC-1 cells were treated with ursolic acid by QRT-PCR; The expression of Bcl-2, Bax and Caspase-9 protein in TPC-1 cells were treated with ursolic acid by Western blot. Results MTT assay showed that ursolic acid inhibited the proliferation of TPC-1 cells in a concentration and time-dependent manner, and the IC50 at 24 h, 48 h and 72 h was 14.21 μM, 10.56 μM, 10.39 μM; Flow cytometry showed that ursolic acid inhibited the apoptosis of TPC-1 cells in a concentration-dependent manner, and the growth of TPC-1 cells was arrested in S phase; QRT-PCR showed that Bcl-2, Bax and Caspase-9 mRNA were expressed in the control and experimental groups, ursolic acid inhibited the expression of Bcl-2 mRNA in a concentration-dependent manner and up-regulated the expression of Bax and Caspase-9 mRNA; Western blot results showed that Bcl-2, Bax and Caspase-9 were expressed in the control and experimental groups, ursolic acid inhibited the expression of Bcl-2 protein in a concentration-dependent manner and up-regulated the expression of Bax protein and Caspase-9 protein.Conclusion Ursolic acid can significantly inhibit the proliferation and induce apoptosis of human papillary thyroid TPC-1 cells, providing some ideas for the treatment of thyroid cancer.
- Thyroid papillary carcinoma,
- Ursolic acid,
- Apoptosis,
- Cycle,
- Bcl-2,
- Bax,
- Caspase-9

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