Changes of Alternative Splice Variant of the SRSF12 by Cisplatin in Multiple Cells

Ma Chun Xia; Li Ming Yang; Wu Han Xin; Yang Yuan; Yang Meng Li; Yu Jian Kun

Article Contents

Article Navigation > Journal of Kunming Medical University > 2018 > 39(01): 5-9

Ma Chun Xia , Li Ming Yang , Wu Han Xin , Yang Yuan , Yang Meng Li , Yu Jian Kun . Changes of Alternative Splice Variant of the SRSF12 by Cisplatin in Multiple Cells[J]. Journal of Kunming Medical University, 2018, 39(01): 5-9.

Citation:

Ma Chun Xia , Li Ming Yang , Wu Han Xin , Yang Yuan , Yang Meng Li , Yu Jian Kun . Changes of Alternative Splice Variant of the SRSF12 by Cisplatin in Multiple Cells[J]. Journal of Kunming Medical University, 2018, 39(01): 5-9.

Citation:

PDF( 744 KB)

Changes of Alternative Splice Variant of the SRSF12 by Cisplatin in Multiple Cells

Funds:

基金：中国医学科学院医学与健康科技创新工程重大协同创新基金资助项目 (2016-12M-2-001); 国家自然科学基金资助项目 (31570918);

Received Date: 2017-06-13

Abstract

Abstract

Objective To detect the changes of mRNA isoforms in multiple cancer cell lines with dose of cisplatin. Methods Total RNA of the cells treated by cisplatin were abstracted 24 h after the treatment. mRNA isoforms of SRSF12 gene were detected by semiquantitative RT-PCR. The ratios of mRNA isoforms were analyzed by gel image software and statistical analysis. Results Under the cisplatin treatment, 2 mRNA isoforms of SRSF12 were detected in five cells except Caski cells, their ratios and relative mRNA levels were changed. With the increase of dose of cisplatin, the ratio of isoform-a was slightly increased; but the ratio of isoform-b was different, the changes were not obvious in the A549 and 293 FT cells, the weaker expression was expressed in the H1299 and C33 A cells, and the two isoforms were gradually weakening in the Siha cells. Conclution Under the cisplatin treatment in multiple cancer cells, the expression of SRSF12 shows tissue-specific and cell type-specific patterns.
- CDDP,
- Arginine-serine rich splicing factor12,
- mRNA alternative splicing (AS) ,
- Tissue-specific,
- Cell type-specific

FullText(HTML)

References(11)

References

[1]	[1]KIM E, GOREN A, AST G.Insights into the connection between cancer and alternative splicing[J].Trends in Genetics, 2008, 24 (1) :7-10.
[2]	[2]DAS S, KRAINER A R.Emerging functions of SRSF1, splicing factor and oncoprotein, in RNA metabolism and cancer.[J].Molecular Cancer Research Mcr, 2014, 12 (9) :1195-1204.
[3]	[3]TWYFFELS L, AL E, SHUTTLING S R.Proteins:more than splicing factors[J].Febs Journal, 2011, 278 (18) :3246-3255.
[4]	[4]LIU H X, ZHANG M, KRAINER A R.Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins[J].Genes Dev, 1998, 12 (13) :1998-2012.
[5]	[5]HERTEL K J, GRAVELEY B R.RS.Domains contact the pre-m RNA throughout spliceosome assembly[J].Trends Biochem Sci, 2005, 30 (3) :115-118.
[6]	[6]VAN D H V O W, NEWTON K, SCREATON G R, et al.Role of SR protein modular domains in alternative splicing specificity in vivo[J].Nucleic Acids Research, 2000, 28 (24) :4822-4831.
[7]	[7]COWPER A E, CCERES J F, MAYEDA A, et al.Serine-arginine (SR) protein-like factors that antagonize authentic SR proteins and regulate alternative splicing[J].Journal of Biological Chemistry, 2001, 276 (52) :48908-48914.
[8]	[8]LI Y, LIN Y, LI Q, et al.SRrp35 suppresses cell proliferation and malignancy in hepatocellular carcinoma[J].Hepatology Research the Official Journal of the Japan Society of Hepatology, 2015, 45 (12) :1241-1247.
[9]马春霞, 杨梦莉, 李明阳, 等.顺铂处理下肺癌细胞剪接因子SRSF1 m RNA异构体的变化[J].贵州医科大学学报, 2017, 42 (8) :879-884.
[10]	[10]BOEHME K A, BLATTNER C.Regulation of p53--insights into a complex process[J].Critical Reviews in Biochemistry&Molecular Biology, 2009, 44 (6) :367-392.
[11]	[11]TOKINO T, NAKAMURA Y.The role of p53-target genes in human cancer.[J].Critical Reviews in Oncology/hematology, 2000, 33 (1) :1-6.

Relative Articles(20)

Relative Articles

[1]	You LI, Heng DING, Liang CUI, Yuanxi ZHAO, Enyu ZHAN, Xingguo LI. The Analysis of the Therapeutic Effect of Intervertebral Foramen Endoscopy on Single Segment Non-specific Lumbar Intervertebral Space Infection. Journal of Kunming Medical University, 2024, 45(5): 94-102. doi: 10.12259/j.issn.2095-610X.S20240515
[2]	Lixiu XU, TUERXUN Meiliban, YAKUFU Kereman. Studies on Cisplatin Resistance of miR-181a in Ovarian Cancer Cells. Journal of Kunming Medical University, 2022, 43(1): 20-25. doi: 10.12259/j.issn.2095-610X.S20220131
[3]	Taibang CHEN, Jing WANG, Di DU, Xiaoqing HE, Wenbao ZHOU. Virtual Reality Distraction Induces Hypoalgesia in Patients with Chronic Nonspecific Low Back Pain. Journal of Kunming Medical University, 2022, 43(9): 59-65. doi: 10.12259/j.issn.2095-610X.S20220910
[4]	Li Zeng Ming , Bao Wen Li . . Journal of Kunming Medical University, 2020, 41(01): 137-140.
[5]	Hu Wan Qin , Zhao Hong Bo , Liang Hong , Zhao Qing Hua , Yang Li Hua . Analysis of Differential Expression Genes and Signal Pathways Associated with Cisplatin Resistant Ovarian Cancer. Journal of Kunming Medical University, 2017, 38(06): 97-101.
[6]	Hu Yu Chong , Lu Jing Kun , Cui Meng Yao . . Journal of Kunming Medical University, 2017, 38(03): 27-30.
[7]	An Yuan . Effect of Blood Purification on Septic Encephalopathy Neuron-specific Enolase. Journal of Kunming Medical University,
[8]	Huang Zeng Jie . Species Specificity of Male Human's 29 Y-SNP Loci. Journal of Kunming Medical University,
[9]	Lu Xi Wei . Clinical Effects of Cisplatin Combined with Temozolomide and Radiotherapy in Treatment of Glioblastomas after Surgery. Journal of Kunming Medical University,
[10]	Chen Nan . Observation on the Clinical Efficacy between Pemetrexed Plus Cisplatin and Paclitaxel Plus Cisplatin in the Treatment of Advanced Non-small Eell Lung Cancer. Journal of Kunming Medical University,
[11]	Yang Jing . . Journal of Kunming Medical University,
[12]	Qiu Jian Wu . . Journal of Kunming Medical University,
[13]	Shen Hong . . Journal of Kunming Medical University,
[14]	Wang Zi Rui . . Journal of Kunming Medical University,
[15]	Hu Yue Xin . . Journal of Kunming Medical University,
[16]	Chen Mei Ni . . Journal of Kunming Medical University,
[17]	Li Heng . . Journal of Kunming Medical University,
[18]	Huang Jie . . Journal of Kunming Medical University,
[19]	. The Curative Effect of Cisplatin Concurrent Chemotherapy and Radiotherapy on Local Advanced Cervical Cancer. Journal of Kunming Medical University,
[20]	. The Inhibitory Effect and Mechanism of Artemether and Cisplatin on Lewise Lung Cancer Bearing C57BL/6J Mice. Journal of Kunming Medical University,