The Expression Changes of JNK3 and APP Protein in Apoptosis of Retinal Ganglion Cells after Optic Nerve Injury

Zhao Yu; Zhao An Qi; Chen Chen; Wang Lin Lin

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Zhao Yu , Zhao An Qi , Chen Chen , Wang Lin Lin . The Expression Changes of JNK3 and APP Protein in Apoptosis of Retinal Ganglion Cells after Optic Nerve Injury[J]. Journal of Kunming Medical University, 2018, 39(02): 25-29.

Citation:

Zhao Yu , Zhao An Qi , Chen Chen , Wang Lin Lin . The Expression Changes of JNK3 and APP Protein in Apoptosis of Retinal Ganglion Cells after Optic Nerve Injury[J]. Journal of Kunming Medical University, 2018, 39(02): 25-29.

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The Expression Changes of JNK3 and APP Protein in Apoptosis of Retinal Ganglion Cells after Optic Nerve Injury

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基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2015FB013); 云南省教育厅科学研究基金资助项目 (2014Z073);

Received Date: 2017-09-12

Abstract

Abstract

Objective To investigate the changes in the expression of JNK3 and APP protein in retinal ganglion cells (RGCs) after optic nerve injury and its effect on the survival of RGCs. Me thods Optic nerve crush models were established using adult APP wild type mice. Immunohistochemistry and western blot for detecting the expression of JNK3 and APP protein in RGCs were used post-injury. The number of RGCs in APP KO mice group was counted to compare with APP WT mice group. Re s ults After optic nerve injury, JNK3 expressed in cytoplasm of RGCs; p-JNK transformed from cytoplasm to nuclei of RGCs; APP expressed in cytomembrane; p-APP expressed in cytoplasm and axons of RGCs. Western blot results showed that the protein expressions of JNK3, p-JNK, APP and p-APP were increased in the injured group (P<0.01) . The survival number of RGCs (147±7.0) in APP KO mice group increased significantly than APP WT mice group (127.7 ±7.0) (P <<0.05) .Conclus ion The upregulation of JNK3 and APP protein expression maybe play an important role in RGCs survival proceeds after optic nerve injury.
- JNK3,
- Amyloid precursor protein,
- Retinal ganglion cells

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References(16)

References

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