Research on Preparation and in Vitro Release of pH Sensitive Gel for Eye Cornea of Salvianolic Acid B

Ke Jin; Zhao Zi Wei; Li Shan Shan; Zhu Pei Fang; Hou An Guo

Article Contents

Article Navigation > Journal of Kunming Medical University > 2018 > 39(03): 23-30

Ke Jin , Zhao Zi Wei , Li Shan Shan , Zhu Pei Fang , Hou An Guo . Research on Preparation and in Vitro Release of pH Sensitive Gel for Eye Cornea of Salvianolic Acid B[J]. Journal of Kunming Medical University, 2018, 39(03): 23-30.

Citation:

Ke Jin , Zhao Zi Wei , Li Shan Shan , Zhu Pei Fang , Hou An Guo . Research on Preparation and in Vitro Release of pH Sensitive Gel for Eye Cornea of Salvianolic Acid B[J]. Journal of Kunming Medical University, 2018, 39(03): 23-30.

Citation:

PDF( 1011 KB)

Research on Preparation and in Vitro Release of pH Sensitive Gel for Eye Cornea of Salvianolic Acid B

Funds:

基金：云南省科技厅应用基础研究基金资助项目 (2014FZ089); 云南省科技厅-云南中医学院应用基础研究联合专项基金资助项目 (2015FB205-003); 云南省重点实验培育计划 (2017DG006);

Received Date: 2017-09-14

Abstract

Abstract

Objective To prepare pH sensitive gel for eye cornea of salvianolic acid B and accomplish in vitro release study. Me thods We used corneal permeability, eye irritation in rabbits, hydration of the cornea, viscosity, gel capacity, transparency, liquidity as index, to select pH sensitive gel matrix composition. Re s ults The gel composition was: 0.5% of salvianolic acid B, 0.14% Carbomer, 0.3% HPMC, 0.05% Azone, 0.1% ethylparaben and 0.2% Vc. The corneal hydration level was 77.32±1.88.The gel released 27.21% drug in 15 min, and released 97.44% drug in 6 h. By research of corneal permeability, the cumulative release of gel was bigger than the one of saline (P<0.05) . Conclus ions The selection of the gel matrix prescription is reasonable in design, simple in process, not harsh to eyes, and can prolong the retention time of drug in corneas and improve the corneal penetration.
- Alvianolic acid B,
- pH sensitive gel for eye cornea,
- Corneal permeability,
- In-vitro release

FullText(HTML)

References(19)

References

[1]李德馨, 王思玲, 苏德森.白内障的发病机制与药物治疗[J].沈阳药科大学学报, 2002, 19 (4) :300-306.
[2]杨玲, 朱莉静, 宋波.66例白内障青光眼联合手术的临床观察[J].昆明医科大学学报, 2014, 35 (3) :136-138.
[3]田影, 何克江, 朱靖博.丹酚酸B的体外抗氧化活性[J].大连工业大学学报, 2008, 27 (4) :3-7.
[4]冷非.丹酚酸B滴眼剂对大鼠糖尿病性白内障的影响[J].哈尔滨医科大学学报, 2009, 43 (4) :249-253.
[5]吴升辉, 王燕凤, 张晓琴.丹酚酸B对糖尿病大鼠角膜的保护作用及其机制[J].国际眼科杂志, 2016, 16 (9) :1629-1631.
[6]赵先, 王婧雯, 陆杨.丹酚酸B药理作用的研究进展[J].西北药学杂志, 2015, 30 (1) :107-110.
[7]	[7]ROBISON JR ED.Ophthalmic Drug Delivery Systems[M].Washington DC:American Pharmaceutical Association, 1980:1.
[8]刘玉生, 薛晓, 李实, 等.环孢素A眼用p H敏感型乳剂凝胶的角膜滞留性及药效学[J].中国新药杂志, 2015, 24 (2) :199-201.
[9]	[9]SAFRTONE MF, CHETONI P, CERBAI R, et a1.Evaluation of ocular permeation enhancers:in vitro effects on corneal transport of fourβ-blockers, and in vitro/in vivo toxic activity[J].Int J Pharm, 1996, 142 (1) :103-113.
[10]吴纯洁.葛根素眼用新型给药系统的研究[D].成都:四川大学, 2005.
[11]张治强, 张玮, 陈光, 等.p H敏感型盐酸左氧氟沙星眼用即型凝胶的制备及其体外释放考察[J].药学实践杂志, 2010, 28 (2) :122-125.
[12]张婧, 李翔, 罗云, 等.姜黄素眼用脂质体的角膜透过性研究[J].江西中医药大学学报, 2015, 27 (2) :79-81.
[13] 李英.甲砜霉素p H敏感眼用凝胶的研究[D].沈阳:沈阳药科大学硕士论文, 2006:26.
[14]邓翔, 何文.司帕沙星眼用p H敏感原位凝胶的制备及体外角膜透过性考察[J].医药导报, 2012, 31 (6) :783-785.
[15]赵雯, 刘睿, 孙璐, 等.3种环糊精对姜黄素角膜渗透性影响研究[J].中草药, 2015, 46 (2) :201-206.
[16]卢召战, 朱靖博, 刘天赐.p H及添加剂对丹酚酸B水溶液稳定性的影响[J].大连工业大学学报, 2008, 27 (3) :209-211.
[17]黄世超, 瞿海斌.丹酚酸B稳定性研究进展[J].中国现代应用药学, 2015, 32 (5) :644-648.
[18]李英.甲砜霉素p H敏感眼用凝胶的研究[D].沈阳:沈阳药科大学 (硕士论文) , 2006:26.
[19]徐亚静, 胡容峰.黄芩苷p H敏感眼用原位凝胶剂的制备与评价[J].辽宁中医药大学学报, 2015, 17 (12) :39-42.

Relative Articles(20)

Relative Articles

[1]	Yongfei LI, Yunhua ZHANG, Wenfen GAO, Jihua LIU, Anbin LIU, Yuena YANG. Uncertainty Evaluation of the Determination of the Content of Ambroxol Hydrochloride Injection by Using TOP-DOWN Method. Journal of Kunming Medical University, 2024, 45(12): 1-5.
[2]	Yongfei LI, Wenfen GAO, Jihua LIU, Yunhua ZHANG, Anbin LIU, Yuena YANG. Uncertainty Evaluation of the Determination of the Content of Ambroxol Hydrochloride Injection by Using TOP-DOWN Method. Journal of Kunming Medical University, 2024, 45(12): 1-5.
[3]	Wenni MA, Wenjuan FENG, Zilin MA, Yongshi FAN, Yi MA, Ying HUANG. The Mediating Effect of Self-management on the Discharge Readiness and Quality of Life in Patients with Type B Aortic Dissection. Journal of Kunming Medical University, 2024, 45(5): 195-200. doi: 10.12259/j.issn.2095-610X.S20240530
[4]	Bing-cheng WU, Hong-mei DAI, Qi-zhi ZHOU, Peng SUN, Lan LI, Dong-fang QI. Phakic Posterior Chamber Intraocular Lens Implantation for Moderate and High Myopia. Journal of Kunming Medical University, 2020, 41(11): 93-97. doi: 10.12259/j.issn.2095-610X.S20201116
[5]	Wu Le Yan , Hou Wen Qing , Sun Kong Chun , Yang Can Yu , Shu Wen , Shen Bao Chun . . Journal of Kunming Medical University, 2020, 41(06): 11-14.
[6]	Zhou Feng Gao , Yang De Xing , Xu Mian , Liu Ya Ming , Xu Cheng , Zhao Kun , Jiang Guo Yun , Liu Rong . . Journal of Kunming Medical University, 2018, 39(10): 51-57.
[7]	Zhang Bing Jie , Zhang Yuan Ping , Ma Lin Kun , Zhou Wei , Zhao Xue Ying , Sun Heng , Wang Li Chao , Guo Xiao Yan . . Journal of Kunming Medical University, 2017, 38(03): 17-20.
[8]	Ke Jin , Cheng Xin , Hou An Guo . Preparation and in Vitro Release Study of Panax Notoginseng Sustained-release Pellets. Journal of Kunming Medical University, 2017, 38(09): 24-29.
[9]	Zeng Hui Juan . Analysis of Amino Acids and Acylcarnitines in the Blood of Patients First Diagnosed with Uremia Using Tandem Mass Spectrometry. Journal of Kunming Medical University,
[10]	Chen Bo . The Expression of Dicer， miR-10b and Its Precursor in Breast Cancer. Journal of Kunming Medical University,
[11]	Zhang Mei Qiong . . Journal of Kunming Medical University,
[12]	Wu Yang . . Journal of Kunming Medical University,
[13]	Feng Qing . . Journal of Kunming Medical University,
[14]	Chen Xi . . Journal of Kunming Medical University,
[15]	Hu Na . . Journal of Kunming Medical University, 2013, 34(03): 1-1.
[16]	Wang Lin . One Case Analysis of Ex-vivo Liver Resection Combined with Partial Liver Autotransplantation for Hepatocellular Carcinoma. Journal of Kunming Medical University,
[17]	Sun Jian . Thermosensitive in Situ Gel Based on Poloxamer System Containing β-cyclodextrin for Sustained Release of Propolis in Vitro. Journal of Kunming Medical University,
[18]	. The Efficacy Evaluation of Extracorporeal Shock Wave and High-frequency Hyperthermia in Treatment of Ⅲ B-prostatitis. Journal of Kunming Medical University,
[19]	Liu Jie . . Journal of Kunming Medical University,
[20]	. Determination of Ondansetron in Rats Plasma by HPLC. Journal of Kunming Medical University,