Osteoking Promotes the Proliferation of BMSC through Activating TGF-β/Smad Signaling Pathway in Castrate Osteoporosis Rats

Zheng Hong; Li Jin Tao; Yuan Xin; Wu Chao; Zeng He; Zhao Hong Bin; Tang Wei

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Zheng Hong , Li Jin Tao , Yuan Xin , Wu Chao , Zeng He , Zhao Hong Bin , Tang Wei . Osteoking Promotes the Proliferation of BMSC through Activating TGF-β/Smad Signaling Pathway in Castrate Osteoporosis Rats[J]. Journal of Kunming Medical University, 2018, 39(03): 5-10.

Citation:

Zheng Hong , Li Jin Tao , Yuan Xin , Wu Chao , Zeng He , Zhao Hong Bin , Tang Wei . Osteoking Promotes the Proliferation of BMSC through Activating TGF-β/Smad Signaling Pathway in Castrate Osteoporosis Rats[J]. Journal of Kunming Medical University, 2018, 39(03): 5-10.

Citation:

Zheng Hong , Li Jin Tao , Yuan Xin , Wu Chao , Zeng He , Zhao Hong Bin , Tang Wei . Osteoking Promotes the Proliferation of BMSC through Activating TGF-β/Smad Signaling Pathway in Castrate Osteoporosis Rats[J]. Journal of Kunming Medical University, 2018, 39(03): 5-10.

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Osteoking Promotes the Proliferation of BMSC through Activating TGF-β/Smad Signaling Pathway in Castrate Osteoporosis Rats

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基金：国家自然科学基金资助项目 (81460647); 云南省应用基础研究计划项目 (2014FB033;2017FE-014&-016);

Received Date: 2017-10-21

Abstract

Abstract

Objective To study the effect of OK on the differentiation of bone marrow stromal stem cells (BMSCs) through the TGFβ/Smad signaling pathway in rats. Methods We removed the bilateral ovaries of rats to replicate OP model, and isolated and cultured BMSCs. BMSCs isolated from nomal rats were cultured as control group, BMSCs isolated from OP rats were divided into 5 groups, OP model group was regularly cultured, positive control group was treated with alendronate sodium (1 μmol/L) , low, medium and high OK treatment group were treated with 50 mL/L, 100 mL/L and 200 ml/L OK respectively.After 24 h incubation, all cells were collected. The proliferation rate of BMSCs was determined by MTT method, and ELISA method was employed to detect the contents of bone formation markers, RT-qPCR was used to determine the m RNA expression level of TGFβ and the protein expression levels of TGFβ, p-Smad2/3 and of Smad2/3 were detected by Western blot. Results Compared with control group, the proliferation rate of the BMSCs in OP model group was reduced, concentrations of bone formation markers (OC, PINP and BALP) were reduced, m RNA and protein expression levels of TGFβ, as well as the phosphorylated level of Smad2/3 were downregulated.The difference was statistically significant (P<0.05) . After treatment with OK, compared with model group, all the above effects were ameliorated in different degree, a dose dependent manner was observed in OK treatment group, and the treatment effects of alendronate sodium (1 μmol/L) , 100 mL/L and 200 mL/L OK group were statistically significant (P <0.05) .Conclusion OK can promote the proliferation of osteoblasts by activating TGFβ/Smad signaling pathway to achieve the effect of treating postmenopausal OP.
- Osteoking,
- Osteoporosis,
- Bone marrow stromal stem cells,
- TGFβ/Smad signaling pathway

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References(15)

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