Prokaryotic Expression and Purification of GST-SRY Fusion Protein

Zhang Xiao Yu; Duan Jun Jun; Wang Jiang; Jia Xiao; Wang Xiao Bo; He Ying Hong

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Zhang Xiao Yu , Duan Jun Jun , Wang Jiang , Jia Xiao , Wang Xiao Bo , He Ying Hong . Prokaryotic Expression and Purification of GST-SRY Fusion Protein[J]. Journal of Kunming Medical University, 2018, 39(04): 1-4.

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Zhang Xiao Yu , Duan Jun Jun , Wang Jiang , Jia Xiao , Wang Xiao Bo , He Ying Hong . Prokaryotic Expression and Purification of GST-SRY Fusion Protein[J]. Journal of Kunming Medical University, 2018, 39(04): 1-4.

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Prokaryotic Expression and Purification of GST-SRY Fusion Protein

Zhang Xiao Yu ^1,2,
Duan Jun Jun ^1,2,
Wang Jiang ^1,2,
Jia Xiao ^1,2,
Wang Xiao Bo ^1,2,
He Ying Hong ^1,2

Funds:

基金：国家自然科学基金资助项目 (31360288);

Received Date: 2017-11-20

Abstract

Abstract

Objective To construct GST-SRY fusion protein expression vector and induce its expression in E.coli, then purificate so as to obtain GST-SRY fusion protein. Me thods SRY gene was amplified by PCR and cloned into prokaryotic expression vector p GEX-6 P-1. p GEX-6 P-1-SRY plasmid was verified by restriction enzyme digestion and DNA sequencing. After that, the recombinant plasmid was transformed into E.coli Rosetta DE3.Expression of GST-SRY fusion protein was induced by IPTG, which was purified by Glutathione Sepharose later on.Re s ults Restriction enzyme digestion and sequencing demonstrated that p GEX-6 P-1-SRY was successfully obtained. The result of SDS-PAGE assay indicated that SRY was successfully expressed in E.coli Rosetta DE3 and expressed a 49 KD SRY protein.Conclus ion The successful construction of SRY gene recombinant prokaryotic expression vector and expression of SRY protein will be helpful for further studies of SRY gene function.
- SRY,
- DNA recombination,
- Gene expression,
- Protein purification

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References(15)

References

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