Regulation of Scutellarin on PKCε in Human Cardiac Microvascular Endothelial Cells

Chen Chen; Wang You Lan; Liu Wei Jun; Rao Qiang; Du Ting Yan; Du Xiao Hua; Yang Wei Min; Zhu Xiu Fen

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Chen Chen , Wang You Lan , Liu Wei Jun , Rao Qiang , Du Ting Yan , Du Xiao Hua , Yang Wei Min , Zhu Xiu Fen . Regulation of Scutellarin on PKCε in Human Cardiac Microvascular Endothelial Cells[J]. Journal of Kunming Medical University, 2018, 39(04): 11-15.

Citation:

Chen Chen , Wang You Lan , Liu Wei Jun , Rao Qiang , Du Ting Yan , Du Xiao Hua , Yang Wei Min , Zhu Xiu Fen . Regulation of Scutellarin on PKCε in Human Cardiac Microvascular Endothelial Cells[J]. Journal of Kunming Medical University, 2018, 39(04): 11-15.

Citation:

Chen Chen , Wang You Lan , Liu Wei Jun , Rao Qiang , Du Ting Yan , Du Xiao Hua , Yang Wei Min , Zhu Xiu Fen . Regulation of Scutellarin on PKCε in Human Cardiac Microvascular Endothelial Cells[J]. Journal of Kunming Medical University, 2018, 39(04): 11-15.

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Regulation of Scutellarin on PKCε in Human Cardiac Microvascular Endothelial Cells

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基金：国家自然科学基金资助项目 (81560589, 30960450, 81173110); 云南省科技厅联合专项基金资助项目[ (2014FA010, 2017FE467 (-019) ];

Received Date: 2018-01-12

Abstract

Abstract

Objective To investigate the regulation of scutellarin (SCU) on protein and m RNA expression of PKCε in Human cardiac microvascular endothelial cells (HCMECs) induced by hypoxia reoxygenation or normal. Me thods We cultured HCMECs in two ways: normal and hypoxia reoxygenation (HR) . The normal groups were divided into one control group and three SCU (0.1 μM, 1 μM, 10 μM) groups;HR treatment induded one control group and four groups of HR, HR+ SCU 0.1 μM, HR+SCU 1 μM and HR+SCU 10 μM. Protein and m RNA expression of PKCε was determined by Western blot and RT-PCR respectively.Re s ults In normal cultured, SCU (0.1 μM, 1 μM and 10 μM) significantly up-regulated the protein and m RNA expression of PKCε (P <0.05) , and for p-PKCε, there was an up-regulated tendency. In the HR treatment, the model group had a down regulation tendency, but SCU 10 μM group could significantly up-regulate the protein and m RNA expression of PKCε (P <0.05) , and SCU 10 μM also could significantly up-regulated p-PKCε (P <0.05) . Conclus ion SCU up-regulates the expression of PKCε protein and m RNA in HCMECs induced by hypoxia reoxygenation or normal.
- Scutellarin,
- HCMECs,
- hypoxia reoxygenation,
- PKCε

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References(19)

References

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