Influence of CD151 Inhibition by RNAi on Lung Metastasis in Null Mice Given Tail Veins Injection of Lung Adenocarcinoma Cell Lines A549

Hu Feng Di; Zhang Jian Qiang; Wang Chu; Luo Chun Xiang; Li Ke

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Hu Feng Di , Zhang Jian Qiang , Wang Chu , Luo Chun Xiang , Li Ke . Influence of CD151 Inhibition by RNAi on Lung Metastasis in Null Mice Given Tail Veins Injection of Lung Adenocarcinoma Cell Lines A549[J]. Journal of Kunming Medical University, 2018, 39(04): 21-26.

Citation:

Hu Feng Di , Zhang Jian Qiang , Wang Chu , Luo Chun Xiang , Li Ke . Influence of CD151 Inhibition by RNAi on Lung Metastasis in Null Mice Given Tail Veins Injection of Lung Adenocarcinoma Cell Lines A549[J]. Journal of Kunming Medical University, 2018, 39(04): 21-26.

Citation:

Hu Feng Di , Zhang Jian Qiang , Wang Chu , Luo Chun Xiang , Li Ke . Influence of CD151 Inhibition by RNAi on Lung Metastasis in Null Mice Given Tail Veins Injection of Lung Adenocarcinoma Cell Lines A549[J]. Journal of Kunming Medical University, 2018, 39(04): 21-26.

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Influence of CD151 Inhibition by RNAi on Lung Metastasis in Null Mice Given Tail Veins Injection of Lung Adenocarcinoma Cell Lines A549

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基金：云南省教育厅科学研究基金资助项目 (2015Y181); 云南省肿瘤医院博士科研基金资助项目 (BSJJ201506); 普洱学院校级创新团队基金资助项目 (CXTD) ;普洱学院高层次人才科研启动基金资助项目 (K2017051);

Received Date: 2017-09-09

Abstract

Abstract

Objective To explore the influence of CD151 inhibition by RNA interference on lung metastasis in null mice given tail veins injection of lung adenocarcinoma cell lines A549. METHODS: Constructed CD151 inhibition by RNA interference cell lines A549-sh RNA, A549-sh NC and A549-Blank were injected into the tail veins of BALB/c nude mice. Lung metastasis was measured through naked eye and pathology of HE stain. Re s ults Lung metastasis were 62.5% (5/8) and (4.7±1.5) , 100% (7/7) and (9.3±0.8) , 75% (6/8) and (8.2±1.5) , respectively in A549-sh RNA, A549-sh NC and A549-Blank. There were differences between A549-sh RNA and A549-sh NC (P = 0.000) and between A549-sh RNA and A549-Blank (P = 0.006) .Conclus ions Inhibition of CD151 gene expression can reduce lung metastasis in null mice given tail veins injection of lung adenocarcinoma cell lines A549. CD151 may become one of the potential therapeutic targets aiming at inhibitung invasion and metastasis of NSCLC.
- CD151,
- Non-small cell lung cancer,
- BALB/c-nude mice,
- Lung metastasis

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References(12)

References

[1]石远凯, 孙燕, 于金明, 等.中国晚期原发性肺癌诊治专家共识 (2016年版) [J].中国肺癌杂志, 2016, 18 (01) :1-15.
[2]	[2]SADEJ R, GRUDOWSKA A, TURCZYK L, et al.CD151in cancer progression and metastasis:A complex scenario[J].Lab Invest, 2014, 94 (1) :41-51.
[3]李科, 洪志鹏, 李云霞, 等.非小细胞肺癌组织CD151蛋白表达临床意义探讨[J].中华肿瘤防治杂志, 2014, 22 (01) :34-38.
[4]李科, 洪志鹏, 沈丽达, 等.CD_ (151) 及整合素α_3β_1蛋白在非小细胞肺癌中的表达及意义[J].中国全科医学, 2014, 16 (05) :531-535.
[5]	[5]LI P, ZENG H, QIN J, et al.Effects of tetraspanin CD151inhibition on A549 human lung adenocarcinoma cells[J].Mol Med Rep, 2015, 11 (2) :1258-1265.
[6]	[6]KUMARI S, DEVI G T, BADANA A, et al.CD151-A Striking Marker for Cancer Therapy[J].Biomark Cancer, 2015, 22 (7) :7-11.
[7]	[7]LIU T, WANG S, WANG L, et al.Targeting CD151 by lentivirus-mediated RNA interference inhibits luminal and basal-like breast cancer cell growth and invasion[J].Mol Cell Biochem, 2015, 407 (1-2) :111-121.
[8]	[8]ZHANG Z, WANG F, LI Q, et al.CD151 knockdown inhibits osteosarcoma metastasis through the GSK-3beta/beta-catenin/MMP9 pathway[J].Oncol Rep, 2016, 35 (3) :1764-1770.
[9]	[9] AZNAVOORIAN S, MURPHY A N, STETLERSTEVENSON W G, et al.Molecular aspects of tumor cell invasion and metastasis[J].Cancer, 1993, 71 (4) :1368-1383.
[10]	[10]FIDLER I J.Critical determinants of metastasis[J].Semin Cancer Biol, 2002, 12 (2) :89-96.
[11]	[11]TAKEDA Y, LI Q, KAZAROV A R, et al.Diminished metastasis in tetraspanin CD151-knockout mice[J].Blood, 2011, 118 (2) :464-472.
[12]	[12]COPELAND B T, BOWMAN MJ, ASHMAN L K.Genetic ablation of the tetraspanin CD151 reduces spontaneous metastatic spread of prostate cancer in the TRAMP model[J].Mol Cancer Res, 2013, 11 (1) :95-105.

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