Influence of CD151 Inhibition by RNAi on Lung Metastasis in Null Mice Given Tail Veins Injection of Lung Adenocarcinoma Cell Lines A549

Hu Feng Di; Zhang Jian Qiang; Wang Chu; Luo Chun Xiang; Li Ke

Volume 39 Issue 04

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Hu Feng Di , Zhang Jian Qiang , Wang Chu , Luo Chun Xiang , Li Ke . Influence of CD151 Inhibition by RNAi on Lung Metastasis in Null Mice Given Tail Veins Injection of Lung Adenocarcinoma Cell Lines A549[J]. Journal of Kunming Medical University, 2018, 39(04): 21-26.

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Hu Feng Di , Zhang Jian Qiang , Wang Chu , Luo Chun Xiang , Li Ke . Influence of CD151 Inhibition by RNAi on Lung Metastasis in Null Mice Given Tail Veins Injection of Lung Adenocarcinoma Cell Lines A549[J]. Journal of Kunming Medical University, 2018, 39(04): 21-26.

Citation:

Hu Feng Di , Zhang Jian Qiang , Wang Chu , Luo Chun Xiang , Li Ke . Influence of CD151 Inhibition by RNAi on Lung Metastasis in Null Mice Given Tail Veins Injection of Lung Adenocarcinoma Cell Lines A549[J]. Journal of Kunming Medical University, 2018, 39(04): 21-26.

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Influence of CD151 Inhibition by RNAi on Lung Metastasis in Null Mice Given Tail Veins Injection of Lung Adenocarcinoma Cell Lines A549

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基金：云南省教育厅科学研究基金资助项目 (2015Y181); 云南省肿瘤医院博士科研基金资助项目 (BSJJ201506); 普洱学院校级创新团队基金资助项目 (CXTD) ;普洱学院高层次人才科研启动基金资助项目 (K2017051);

Received Date: 2017-09-09

Abstract

Abstract

Objective To explore the influence of CD151 inhibition by RNA interference on lung metastasis in null mice given tail veins injection of lung adenocarcinoma cell lines A549. METHODS: Constructed CD151 inhibition by RNA interference cell lines A549-sh RNA, A549-sh NC and A549-Blank were injected into the tail veins of BALB/c nude mice. Lung metastasis was measured through naked eye and pathology of HE stain. Re s ults Lung metastasis were 62.5% (5/8) and (4.7±1.5) , 100% (7/7) and (9.3±0.8) , 75% (6/8) and (8.2±1.5) , respectively in A549-sh RNA, A549-sh NC and A549-Blank. There were differences between A549-sh RNA and A549-sh NC (P = 0.000) and between A549-sh RNA and A549-Blank (P = 0.006) .Conclus ions Inhibition of CD151 gene expression can reduce lung metastasis in null mice given tail veins injection of lung adenocarcinoma cell lines A549. CD151 may become one of the potential therapeutic targets aiming at inhibitung invasion and metastasis of NSCLC.
- CD151,
- Non-small cell lung cancer,
- BALB/c-nude mice,
- Lung metastasis

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References

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