The Experiment of Edaravone on Primary Microglia Activation by Lipopolysaccharide

Qiao Ting Ting; Chen Zhong Yi; Dong Bao Lian; Yin Yan; Guo Ling

Article Contents

Article Navigation > Journal of Kunming Medical University > 2018 > 39(04): 5-10

Qiao Ting Ting , Chen Zhong Yi , Dong Bao Lian , Yin Yan , Guo Ling . The Experiment of Edaravone on Primary Microglia Activation by Lipopolysaccharide[J]. Journal of Kunming Medical University, 2018, 39(04): 5-10.

Citation:

Qiao Ting Ting , Chen Zhong Yi , Dong Bao Lian , Yin Yan , Guo Ling . The Experiment of Edaravone on Primary Microglia Activation by Lipopolysaccharide[J]. Journal of Kunming Medical University, 2018, 39(04): 5-10.

Citation:

PDF( 3812 KB)

The Experiment of Edaravone on Primary Microglia Activation by Lipopolysaccharide

Funds:

基金：国家自然科学基金资助项目 (81760228); 云南省教育厅科学研究基金资助项目 (2012Z093); 云南省科技厅科技计划青年基金资助项目 (2017FD193);

Received Date: 2018-01-11

Abstract

Abstract

Objective To explore the effect of Edaravone (EDA) on oxidative stress such as release of nitric oxide (NO) induced by Lipopolysaccharide (LPS) in activation of primary microglia. Me thods There were four groups including control, LPS, LPS + EDA and MAPK inhibitors + EDA + LPS in this study. The primary mixed glial cells were derived from the cortex of Sprague-Dawley rats which were 2 to 3-day old neonatal pups. The primary microglia were separated and purified by Biological Shaker after growing for about ten days. Griess Assay was used to detect NO secreted by activated microglia in four groups. Re s ults The shake-off microglial cells were confirmed as purity of 95% or up via fluorescence staining of CD68. Compared with the control group, NO product of microglial activation mediated by LPS significantly increased while NO value decreased in the group of EDA which was pretreatedt of EDA ahead of LPS for 24 hrs (P<0.05) . After addition of LPS, the incubation was remained for additional 24 h. In addition to EDA pretreatment for 24 h, the addition of three Mitogen-activated protein kinase inhibitors of MAPK signaling pathways were ahead of addition of EDA for 30 min, and then 10 ng/m Lof LPS was added to microglia. All of them incubated with the primary microglia for 24 h after the last addition. NO release was significantly reduced, the difference was statistically significant (P <0.05) .Conclus ion EDA treatment alone could inhibit the release of NO induced by LPS in primary microglia while EDA plus MAPK signaling pathway inhibitor obviously reduced release of NO. EDA may take effect with p38 MAPK, JNK and ERK inhibitors of sub-pathways in some way (s) to co-operate with EDA in modulating oxidative stress in activated microglia induced by LPS. As to the working mechanisms how EDA co-operates with MAPK inhibitors is still remain unknown.
- Edaravaone,
- Lipopolysaccharide,
- Microglia,
- Nitric oxide

FullText(HTML)

References(27)

References

[1]章玲, 张衡, 孙秉贵.星形胶质细胞在阿尔茨海默病中的作用及其分子机制[J].2017, 29 (5) :501-506.
[2]沈婕, 李久胜.小胶质细胞在神经退行性疾病中的神经免疫调节作用[J].河南医学研究, 2017, 26 (2) :244-246.
[3]谭子健, 巨淑慧, 黄潇, 等.胶质细胞干细胞/前体细胞特性的研究进展[J].生理学报, 2017, 69 (2) :207-217.
[4]王茜, 程雪娇, 李学敏, 等.小胶质细胞在慢性神经退行性疾病中的作用研究进展[J].卫生研究, 2015, 44 (6) :1023-1027.
[5]蒋宏国.过表达Nurr1基因的小胶质细胞对神经干细胞向多巴胺能神经元分化的影响[D].昆明:昆明医科大学硕士论文, 2015.
[6]	[6]ZHANG P, LI W, LI L, et al.Treatment with edaravone attenuates ischemic brain injury and inhibits neurogenesis in the subventricular zone of adult rats after focal cerebral ischemia and reperfusion injury[J].Neuroscience, 2012, 201 (1) :297-306.
[7]邱奥望, 刘展, 郭军, 等.神经炎症与神经退行性疾病的关系[J].生理科学进展, 2011, 42 (5) :253-258.
[8]	[8]GLASS C K, SAIJO K, WINNER B, et al.Mechanisms underlying inflammation in neurodegeneration[J].Cell, 2010, 140 (6) :918-934.
[9]	[9]GUO L, LJ L D M E.A Dual role for apolipoprotein E in neuroinflammation anti-and pro-inflammatory activity[J], Journal of Molecular Neuroscience, 2004, 23 (3) :205-212.
[10]	[10]GUO L, SAWKAR A, ZASADZKI M, et al.Similar activation of glial cultures from different rat brain regions by neuroinflammatory stimuli and downregulation of the activation by a new class of small molecule ligands[J], Neurobiology ofAging, 2001, 22 (6) :975-981.
[11]	[11]RALAY R H, CRAFT J M, HU W, et al.Glia as a therapeutic target:Selective suppression of human amyloid-β-induced upregulation of brain proinflammatory cytokine production attenuates neurodegeneration[J], The Journal ofNeurobiology, 2006, 26 (2) :662-670.
[12]	[12]WATTERSON DM, MIRZOEVA S, GUO L, et al.Ligand modulation of glial activation:Cell permeable, small molecule inhibitors of serine-threonine protein kinases can block induction of interleukin 1βand nitric oxide synthase II[J].Neurochemistry International, 2001, 39 (5-6) :459-468.
[13]	[13]PETROVA TV, AKAMA KT, LINDA J, et al.Cyclopentenone prostaglandins suppress activation of microglia:Down-regulation of inducible nitric-oxide synthase by15-deoxy-△12, 14-prostaglandin J2[J].Neurobiology, 1999, 96 (8) :4668-4673.
[14]	[14]JINGRU H U, FRANCIS CASTETS, JOSE L.GUEVARA, et al.S100βstimulates inducible nitric oxide synthase activity and m RNA levels in rat cortical astrocytes[J].THE JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (5) :2543-2547.
[15]辛岗, 苏芸, 许燕璇, 等.脂多糖活化小鼠大脑小胶质细胞后炎性细胞因子的表达[J].中国热带医学, 2011, 11 (8) :983-988.
[16]袁龙.脂多糖所介导的小胶质细胞活化的分子机制[D].延吉:延边大学硕士论文, 2012.
[17]	[17]ALOISI F, RIA F, ADORINI L.Regulatiol of T-cell responses by CNS antigen-pres enting cells:different roles for microglia and astrocytes[J].Immunology Today, 2000, 21 (3) :141.
[18] 乔廷廷, 郭玲.MAPK信号传导通路调控Amyloid-beta介导神经炎症中的过氧化物[J].昆明医科大学学报, 2016, 37 (2B) :309-313.
[19]姚惠亚, 张连生, 伍进, 等.依达拉奉对急性脑梗死患者神经元特异性烯醇化酶和C反应蛋白水平及预后的影响[J].中国医师杂志, 2012, 14 (3) :411-413.
[20]袁云, 李璠, 韩宏, 等.依达拉奉通过Notch信号通路调节BV-2小胶质细胞激活的研究[J].神经解剖学杂志, 2017, 33 (5) :560-566.
[21]王漫.JNK抑制剂SP600125对AD脑内突触损害和炎性反应治疗作用及其机制研究[D], 西安:第四军医大学硕士论文, 2015.
[22]	[22]AGTHONG S, KOONAMJ, KAEWSEMA A, et al.Inhibition of MAPK ERK impairs axonal regeneration without an effect on neuronal loss after nerve injury[J].Neurol Res, 2009, 25 (10) :1068-1074.
[23]刘婷婷, 张淑萍, 覃筱燕, 等.MAPK信号传导通路与神经损伤研究进展[J].中国公共卫生, 2016, 32 (2) :248-254.
[24]吴晓, 金茂强, 陈含笑.闭合性弥漫性脑损伤后IL-1β.P38MAPK及VCAM-1的动态表达及意义[J].新疆医科大学学报, 2011, 34 (1) :62-65.
[25]	[25]AVRAMOVICH Y, AMIT T, YOUDIM MB.Non-steroidal anti-inflammatory drugs stimulate secretion of non-amyloidogenic precursor protein[J].The Journal of Biological Chemistry, 2002, 277 (35) :31466-31473.
[26]	[26]WANG S D, CHEN B C, KAO S T, et al.Genistein inhibits tumor invasion by suppressing multiple signal transduction pathways in human hepatocellular carcinoma cells[J].BMCComplementary and Alternative Medicine, 2013, 14 (1) :1-26.
[27]	[27]TSAI J P, HSIAO P C, YANG S F, et al.Licochalcone Asuppresses migration and invasion of human hepatocellular carcinoma cells through downregulation of MKK4/JNK via NF-KB mediated urokinase plasminogen activator exprssion[J].PLoS One, 2014, 9 (1) :e86537.

Relative Articles(20)

Relative Articles

[1]	Guojing LIANG, Kun JI, Kaichun ZHANG, Yufang ZHANG, Jing AN, Yuge ZHANG, Juan WEN, Haiyan REN. The Effect of Probiotics on the Expression of Aβ and the Protective Effect of Neurons in Rats with Cerebral Ischemia-Reperfusion Injury. Journal of Kunming Medical University, 2024, 45(5): 37-43. doi: 10.12259/j.issn.2095-610X.S20240506
[2]	Shaoyan NIE, Susu FAN, Yushan ZHU, Xuerong PENG, Yang WANG, Xuan ZHANG. Protective Effects of Xiyanping Injection on Lipopolysaccharide-induced Acute Lung Injury in Mice. Journal of Kunming Medical University, 2024, 45(11): 1-7.
[3]	Yu LV, Yibo HU, Jiaxin WANG, Youtong SU, Youguo DAI. Advances on the Relationship between Protein S-nitrosylation and Digestive System Tumors. Journal of Kunming Medical University, 2022, 43(9): 155-159. doi: 10.12259/j.issn.2095-610X.S20220911
[4]	Kun ZHAO, Yun XIAO, Chun YANG, Zhiling YAN, Minna DONG, Bingquan XIANG, Mingyao XIAO. Protective Effect of Interleukin-4 in Lipopolysaccharide-induced Acute Lung Injury Models. Journal of Kunming Medical University, 2022, 43(8): 41-46. doi: 10.12259/j.issn.2095-610X.S20220805
[5]	Ting-na ZHU, Peng-liang LIU, Wen-juan DONG, Hao YU, Ya-mei WU, Zhao-kui HUANG, Shi-jun HONG, Yong-na ZHAO. Effects of Different Doses of Gastrodin on Conditioned Place Preference and Microglia Activation in Hippocampus of Methamphetamine Dependent Rats. Journal of Kunming Medical University, 2021, 42(5): 6-11. doi: 10.12259/j.issn.2095-610X.S20210502
[6]	Yin Sai Ge , Zhang Heng Rui , Chen Yan , Sun Jun . . Journal of Kunming Medical University, 2019, 40(01): 6-9.
[7]	Chen Zhong Yi , Qiao Ting Ting , Dong Bao Lian , Guo Ling . A Study of LPS Mediated Inflammatory Oxidation Reaction of Three Types of Rodents. Journal of Kunming Medical University, 2018, 39(06): 7-13.
[8]	Fu Xi Yue, Lu Di , Bian Li Gong , Zhou Li . . Journal of Kunming Medical University, 2017, 38(02): 127-130.
[9]	Cao Yong Mei , Lv Yi , Liu Yu Jing , Li Ying Chuan . The Effect of miR-200 and Their Targets ZEB1/2 on Epithelial-mesenchymal Transition(EMT) in Early Pulmonary Fibrosis Caused by Acute Lung Injury. Journal of Kunming Medical University, 2016, 37(12): 1-7.
[10]	Huang Sheng Hua , Li Xiao Xia , Lian Xi Yan , Chen Zi Yao , Zhao Jing Tao , Feng Zhi Jian . Influence of Uric Acid on Human Umbillical Vein Endothelial Cells under Oxidative Stress. Journal of Kunming Medical University, 2016, 37(12): 20-23.
[11]	Tang Tao , Sun Xian Run , Jiang Yan Ping , Zhang Min , Ma Hua , Long Yu . Correlation Between NO/iNOS and Destruction of Articular Cartilage. Journal of Kunming Medical University, 2016, 37(11): 28-32.
[12]	Kui Li Yue . . Journal of Kunming Medical University,
[13]	Wang Hui Ming . . Journal of Kunming Medical University,
[14]	Bian Hai Xia . . Journal of Kunming Medical University,
[15]	Wang Jian . . Journal of Kunming Medical University,
[16]	Yao Jie Mei . . Journal of Kunming Medical University,
[17]	Li Fu Ping . . Journal of Kunming Medical University,
[18]	Zou Zhi Rong . Flavonoid Isolated from the Leaves of Acer Truncatum Bunge Inhibits Lipopolysaccharide-induced Activation of Raw264.7 Cells. Journal of Kunming Medical University,
[19]	. The Quantitative Changes of Brain Microglia Cells of Pure Cerebral Concussion and Multiple Cerebral Concussions in Rats. Journal of Kunming Medical University,
[20]	Tang Mao Dan . . Journal of Kunming Medical University, 2010, 31(01): 1-1.