Tissue Distribution of Yunaconitine in Rats by UPLC-MS/MS Method

Lu Fa Huan; Li Ji Yin; Li Shu Hua; Zhao Wen Song; Wang Rui; Jie Run Fang; Yang Kai Run

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Article Navigation > Journal of Kunming Medical University > 2018 > 39(05): 16-20

Lu Fa Huan , Li Ji Yin , Li Shu Hua , Zhao Wen Song , Wang Rui , Jie Run Fang , Yang Kai Run . Tissue Distribution of Yunaconitine in Rats by UPLC-MS/MS Method[J]. Journal of Kunming Medical University, 2018, 39(05): 16-20.

Citation:

Lu Fa Huan , Li Ji Yin , Li Shu Hua , Zhao Wen Song , Wang Rui , Jie Run Fang , Yang Kai Run . Tissue Distribution of Yunaconitine in Rats by UPLC-MS/MS Method[J]. Journal of Kunming Medical University, 2018, 39(05): 16-20.

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Tissue Distribution of Yunaconitine in Rats by UPLC-MS/MS Method

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基金：云南省科技计划基金资助项目 (2013FB118); 昆明医科大学科技创新团队建设基金资助项目 (CXTD201604); 云南省教育厅科学研究基金资助项目 (2017z DX161);

Received Date: 2018-03-13

Abstract

Abstract

Objective To establish an acute yunaconitine poisoning rat model with a single oral administration and to determine the contents of yunaconitine in rat tissues by UPLC-MS/MS method, then investigate the distribution of yunaconitine in rats. Me thod The rats were randomly divided into three groups and were intragastrically administered a single dose of 2.2 mg/kg, 1.1 mg/kg, 0.7 mg/kg yunaconitine, respectively.. The rats were killed 2 h later, the stomach tissue, intestine tissue, liver tissue, pancreas tissue, kidney tissue, lung tissue, spleen tissue, heart tissue, bladder tissue, testis tissue, brain tissue and heart blood samples were collected. The contents of yunaconitine in the biological materials were determined by UPLC-MS/MS method after the biological samples extracted by liquid-liquid extraction. Re s ult A rat model of the yunaconitine poisoning was made with a single dose of 1.1 mg/kg, the concentrations of yunaconitine displayed in the organs with the following order:stomach, small intestine, liver, pancreas, kidney, lung, spleen, heart, bladder, testis, heart blood and brain. Conclus ion Yunaconitine was widely distributed in rats, especially the levels in the stomach, small intestine and liver were the highest. The conclusion provides a basis for the selection of test materials for the poisoning of Aconitum vilmorinianum Kom.
- Aconitum vilmorinianum Kom,
- Yunaconitine,
- UPLC-MS/MS,
- Rat,
- Tissue distribution

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References(18)

References

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