Expression and Significance of Plasma miR-223-3p in Diabetic Kidney Diseaseat Different Stages

He Jun Yi; Li Lan; Li Rong; Huang Yun Hua; Yang Qiu Ping; Wu Ya Nan; Zhang Li Hua

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He Jun Yi , Li Lan , Li Rong , Huang Yun Hua , Yang Qiu Ping , Wu Ya Nan , Zhang Li Hua . Expression and Significance of Plasma miR-223-3p in Diabetic Kidney Diseaseat Different Stages[J]. Journal of Kunming Medical University, 2018, 39(05): 45-49.

Citation:

He Jun Yi , Li Lan , Li Rong , Huang Yun Hua , Yang Qiu Ping , Wu Ya Nan , Zhang Li Hua . Expression and Significance of Plasma miR-223-3p in Diabetic Kidney Diseaseat Different Stages[J]. Journal of Kunming Medical University, 2018, 39(05): 45-49.

Citation:

He Jun Yi , Li Lan , Li Rong , Huang Yun Hua , Yang Qiu Ping , Wu Ya Nan , Zhang Li Hua . Expression and Significance of Plasma miR-223-3p in Diabetic Kidney Diseaseat Different Stages[J]. Journal of Kunming Medical University, 2018, 39(05): 45-49.

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Expression and Significance of Plasma miR-223-3p in Diabetic Kidney Diseaseat Different Stages

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基金：国家自然科学基金资助项目 (81560126); 云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目[2017FE468 (-042) ]; 云南省应用基础研究基金资助项目 (2016FB127); 成都医学院科研基金资助项目 (CYZ15-08);

Received Date: 2018-02-23

Abstract

Abstract

Objective To explore the expression of miR-223-3 p in the plasma of patients with diabetic kidney disease and its clinical significance. Me thods The expression levels of plasma miR-223-3 p in normoalbuminuric group (DM) , microoalbuminuria group (Micro-DKD) , macroalbuminuria group (Macro-DKD) and healthy controls were measured by quantitative real-time polymerase chain reaction (q RT-PCR) . To analysis the relationship with clinical pathological parameters, target genes of miR-223-3 p were predicted with bioinformatics software. Re s ults The levels of miR-223-3 p in the plasma of the remaining three groups were significantly lower than those in the healthy controls (P <0.001) , and the decrease was positively correlated with the severity of the disease. The potential target genes of miR-223-3 p identified by bioinformatics softwares include IL6 ST and PRKCE. Conclus ion The expressionof miR-223-3 pin diabetic kidney disease (DKD) patients' plasma decreased and was positively correlated with the severity of the disease. It may play an important role in the development and progression of diabetic kidney disease through its target genes.
- Diabetic kidney disease,
- miR-223-3p,
- Target genes,
- Quantitative real-time PCR

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References(24)

References

[1]	[1]BARTEL D P.Micro RNAs:Target recognition and regulatory functions[J].Cell, 2009, 136 (2) :215-233.
[2]	[2]ZHANG L, RONG L, HE J, et al.Co-expression analysis among micro RNAs, long non-coding RNAs, and messenger RNAs to understand the pathogenesis and progression of diabetic kidney disease at the genetic level[J].Methods, 2017, 124 (5) :46-56.
[3] 中华医学会糖尿病学分会.中国2型糖尿病防治指南 (2013年版) [J].中华内分泌代谢杂志, 2014, 30 (10) :447-498.
[4]莫一菲, 周健, 贾伟平.国际糖尿病联盟2012年全球2型糖尿病指南解读[J].中国医学前沿杂志 (电子版) , 2012, 4 (11) :70-77.
[5]	[5]CAON B L, DIAZH N, CALVO HUEROS J I, et al.Incidence of cardiovascular disease and validity of equations of coronary risk in diabetic patients with metabolic syndrome[J].Med Clin (Barc) , 2007, 128 (14) :529-535.
[6] 中华医学会糖尿病学分会微血管并发症学组.糖尿病肾病防治专家共识 (2014年版) [J].中国糖尿病杂志, 2014, 6 (11) :792-801.
[7]	[7]LONG J, WANG Y, WANG W, et al.Micro RNA-29c is a signature micro RNA under high glucose conditions that targets Sprouty homolog 1, and its in vivo knockdown prevents progression of diabetic nephropathy[J].J Biol Chem, 2011, 286 (13) :11837-11848.
[8]	[8]WANG Q, WANG Y, MINTO A W, et al.Micro RNA-377is upregulated and can lead to increased fibronectin production in diabetic nephropathy[J].Faseb J, 2008, 22 (12) :4126-4135.
[9]	[9]KATO M, ARCE L, WANG M, et al.A micro RNA circuit mediates transforming growth factor-beta1 autoregulation in renal glomerular mesangial cells[J].Kidney Int, 2011, 80 (4) :358-368.
[10]	[10]CHUNG A C, LAN H Y.Micro RNAs in renal fibrosis[J].Front Physiol, 2015, 6:50.doi:10.3389/fphys.2015.00050.
[11]袁牧, 王昌留, 王一斐, 等.超氧化物歧化酶的研究进展[J].中国组织化学与细胞化学杂志, 2016, 25 (6) :550-558.
[12]	[12]ZHANG C, WANG C, CHEN X, et al.Expression profile of micro RNAs in serum:A fingerprint for esophageal squamous cell carcinoma[J].Clin Chem, 2010, 56 (12) :1871-1879.
[13]	[13]WANG H, PENG W, OUYANG X, et al.Circulating micro RNAs as candidate biomarkers in patients with systemic lupus erythematosus[J].Transl Res, 2012, 160 (3) :198-206.
[14]	[14]XU J, YAO Q, HOU Y, et al.mi R-223/Ect2/p21 signaling regulates osteosarcoma cell cycle progression and proliferation[J].Biomed Pharmacother, 2013, 67 (5) :381-386.
[15]	[15]HUANG K, DONG X, SUI C, et al.mi R-223 suppresses endometrial carcinoma cells proliferation by targeting IGF-1R[J].Am J Transl Res, 2014, 6 (6) :841-849.
[16]	[16]WALSH C A, QIN L, TIEN J C, et al.The function of steroid receptor coactivator-1 in normal tissues and cancer[J].Int J Biol Sci, 2012, 8 (4) :470-485.
[17]刘秉文, 陈俊杰.医学分子生物学[M].北京:中国协和医科大学出版社, 2006:191-604.
[18]戴国华, 宋宪波, 马培泽, 等.微小RNA-223-3p通过调节Rps6kb1/HIF-1α信号通路抑制缺血心肌微血管内皮细胞血管新生[J].中华心血管病杂志, 2014, 42 (12) :1039-1047.
[19]李星军, 王哲敏, 郭友成, 等.血清VEGF、IL-6、Cys-C水平与2型糖尿病肾脏疾病的关系研究[J].检验医学与临床, 2015, 12 (21) :3207-3208.
[20]	[20]TANAKA T, NARAZAKI M, KISHIMOTO T.IL-6 in inflammation, immunity, and disease[J].Cold Spring Harb Perspect Biol, 2014, 6 (10) :a016295.
[21]	[21]WOLF J, ROSE-JOHN S, GARBERS C.Interleukin-6and its receptors:A highly regulated and dynamic system[J].Cytokine, 2014, 70 (1) :11-20.
[22]	[22]WUEEST S, ITEM F, LUCCHINI F C, et al.Mesenteric fat lipolysis mediates obesity-associated hepatic steatosis and insulin Resistance[J].Diabetes, 2016, 65 (1) :140.
[23]	[23]DEKKER L V, PARKER P J.Protein kinase C--a question of specificity[J].Trends in Biochemical Sciences, 1994, 19 (2) :73.
[24]	[24]BASTA P, STRICKLAND M B, HOLMES W, et al.Sequence and expression of human protein kinase C-epsilon[J].Biochimica et Biophysica Acta (BBA) -Gene Structure and Expression, 1992, 1132 (2) :154-160.

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