Expression of Astrocyte, Neuron, and Proinflammatory Cytokines Derived from Frontal Cortex of Methamphetamine Dependent rats after Gastrodin Administration

Ma Chen Li; Yang Gen Meng; Yan Qing Wen; Zhang Feng Yi; Shi Zhen Jin; Zhou Yi Qing; Wang Yi Hang; Zhao Yong Na; Zhang Dong Xian; Li Li Hua; Hong Shi Jun

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Ma Chen Li , Yang Gen Meng , Yan Qing Wen , Zhang Feng Yi , Shi Zhen Jin , Zhou Yi Qing , Wang Yi Hang , Zhao Yong Na , Zhang Dong Xian , Li Li Hua , Hong Shi Jun . Expression of Astrocyte, Neuron, and Proinflammatory Cytokines Derived from Frontal Cortex of Methamphetamine Dependent rats after Gastrodin Administration[J]. Journal of Kunming Medical University, 2018, 39(06): 1-6.

Citation:

Ma Chen Li , Yang Gen Meng , Yan Qing Wen , Zhang Feng Yi , Shi Zhen Jin , Zhou Yi Qing , Wang Yi Hang , Zhao Yong Na , Zhang Dong Xian , Li Li Hua , Hong Shi Jun . Expression of Astrocyte, Neuron, and Proinflammatory Cytokines Derived from Frontal Cortex of Methamphetamine Dependent rats after Gastrodin Administration[J]. Journal of Kunming Medical University, 2018, 39(06): 1-6.

Citation:

Ma Chen Li , Yang Gen Meng , Yan Qing Wen , Zhang Feng Yi , Shi Zhen Jin , Zhou Yi Qing , Wang Yi Hang , Zhao Yong Na , Zhang Dong Xian , Li Li Hua , Hong Shi Jun . Expression of Astrocyte, Neuron, and Proinflammatory Cytokines Derived from Frontal Cortex of Methamphetamine Dependent rats after Gastrodin Administration[J]. Journal of Kunming Medical University, 2018, 39(06): 1-6.

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Expression of Astrocyte, Neuron, and Proinflammatory Cytokines Derived from Frontal Cortex of Methamphetamine Dependent rats after Gastrodin Administration

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基金：云南省教育厅科学研究基金资助项目 (2016YJS051); 国家自然科学基金资助项目 (81160380, 81560302, 81760337); 云南省科技厅-昆明医科大学联合专项资助项目 (2013FB123, 2017FE467-003); 昆明医科大学科技创新团队项目 (CXTD201604);

Received Date: 2018-04-13

Abstract

Abstract

Objective To observe the curative mechanism and effect of neurotoxicity injury induced by methamphetamine (MA) and the neuroprotective effects of gastrodin interfered. Whether the expression of astrocyte and proinflammatory cytokines has contributed to the effects of gastrodin.Me thods 48 healthy male SD rats were randomly divided into three groups: control group (Daily intraperitoneal injection of saline for 8 weeks) , MA group (A dose of 10 mg/kg MA was administered every day for four weeks, then given daily intraperitoneal injection with10 mg/kg saline for 4 weeks) and gastrodin group (A dose of 10 mg/kg MA was administered every day for four weeks, then given daily intraperitoneal injection with 10 mg/kg gastrodin for 4 weeks) . The behavioral changes of rats were measured by conditioned place preference ( CPP) and sterotyped behavior ( SB) induced by methamphetamine. Immunofluorescence staining was used to detect the expression of glial fibrillary acidic protein (GFAP) and NEUN in rat frontal cortex.The expression of IL-6 and TNF-α were detected by quantity RT-PCR and westrn bloting.Re s ults Compa MA depndent 4 weeks group with control group, the scores of sterotyped behavior of MA depndent groups had signficantly increased (P <0.01) . Comparing MA depndent 4 weeks group with MA depndent 4 weeks+gastrodin group, the scores of sterotyped behavior of MA dependent 4 weeks group had obviously decreaseed (P<0.01) . Compared with the control group, the expression of GFAP of MA dependent 4 weeks group decreased and the expression of NEUN increased. Compared MA dependent 4 weeks group with control group, the expression of IL-6 and TNF-α increased (P < 0.01) . Compared MA dependent 4 weeks+gastrodian group with MA dependent 4 weeks group, the expression of TNF-α and IL-6 significantly reduced (P < 0.01) .Conclus ion The neurological damage induced by methamphetamine might be related to the activation of astrocytes and the high expression of inflammatory cytokines including IL-6 and TNF-α. Gastrodin could abate the neurological injury of methamphetamine dependence via reducing the activation of astrocytes and decreasing the expression of IL-6 and TNF-α.
- Gastrodin,
- Methamphetamine,
- Astrocyte,
- Neuron,
- Proinflammatory cytokines

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References(16)

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