A Study on Methylation Status of Imprinted Gene H19 and PEG1 in Different Quality of Sperms and Embryos

Xiang Li Feng; Wang Qing Qing; Li Ming Ying; Zeng Peng; Li Yong Gang

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Xiang Li Feng , Wang Qing Qing , Li Ming Ying , Zeng Peng , Li Yong Gang . A Study on Methylation Status of Imprinted Gene H19 and PEG1 in Different Quality of Sperms and Embryos[J]. Journal of Kunming Medical University, 2018, 39(06): 101-104.

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Xiang Li Feng , Wang Qing Qing , Li Ming Ying , Zeng Peng , Li Yong Gang . A Study on Methylation Status of Imprinted Gene H19 and PEG1 in Different Quality of Sperms and Embryos[J]. Journal of Kunming Medical University, 2018, 39(06): 101-104.

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A Study on Methylation Status of Imprinted Gene H19 and PEG1 in Different Quality of Sperms and Embryos

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基金：云南省卫生科技计划基金资助项目 (2014NS255);

Received Date: 2018-04-12

Abstract

Abstract

Objective By comparing the methylation status of imprinted gene H19 in three different quality spermatozoa and the methylation status of the embryos after the combination of spermatozoa, the sperm methylation status in the ART technique was preliminarily explored with the postnatal embryo methylation.Me thods In this study, a total of 91 spermatozoa and 91 low-quality embryos were collected from IVF-ET in the First People's Hospital of Yunnan Province. Among them, sperm samples were divided into three groups according to the sperm concentration parameter in the WHO fourth edition, then the relationship between different quality spermatozoa and postnatal embryo methylation were analyzed.Re s ults The abnormality rate of H19 methylation status in group B was significantly higher than that of the sperm group and the embryo group.Conclus ions The abnormalities of H19 methylation mainly concentrated in the abnormal parameters of spermatozoa, suggesting that the abnormal state was related to the decrease of sperm quality;Through IVF, ICSI fertilized embryos have the same proportion of the corresponding abnormal state with no significant difference; Abnormal sperm embryos did not show abnormality, which may be related to the self-repair function of the embryo itself.
- Imprinting gene H19,
- Sperm,
- Embryo,
- Methylation,
- ART

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References(12)

References

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