Analysis of the Carbapenemase Genotype in the Carbapenem-resistant Acinetobacter Baumannii in Traumatology Department

Deng De Yao; Yuan Wen Li; Wu Guang Juan; Chen Di; Li Li Li; Gao Zong Ying; Liu Chang; Gu Jin Yi

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Article Navigation > Journal of Kunming Medical University > 2018 > 39(06): 113-117

Deng De Yao , Yuan Wen Li , Wu Guang Juan , Chen Di , Li Li Li , Gao Zong Ying , Liu Chang , Gu Jin Yi . Analysis of the Carbapenemase Genotype in the Carbapenem-resistant Acinetobacter Baumannii in Traumatology Department[J]. Journal of Kunming Medical University, 2018, 39(06): 113-117.

Citation:

Deng De Yao , Yuan Wen Li , Wu Guang Juan , Chen Di , Li Li Li , Gao Zong Ying , Liu Chang , Gu Jin Yi . Analysis of the Carbapenemase Genotype in the Carbapenem-resistant Acinetobacter Baumannii in Traumatology Department[J]. Journal of Kunming Medical University, 2018, 39(06): 113-117.

Citation:

Deng De Yao , Yuan Wen Li , Wu Guang Juan , Chen Di , Li Li Li , Gao Zong Ying , Liu Chang , Gu Jin Yi . Analysis of the Carbapenemase Genotype in the Carbapenem-resistant Acinetobacter Baumannii in Traumatology Department[J]. Journal of Kunming Medical University, 2018, 39(06): 113-117.

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Analysis of the Carbapenemase Genotype in the Carbapenem-resistant Acinetobacter Baumannii in Traumatology Department

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基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2015FB081); 云南省教育厅科学研究基金资助项目 (2014Z071);

Received Date: 2018-02-21

Abstract

Abstract

Objective To investigate the genotype of D class of carbapenemase of carbapenem-resistant Acinetobacter baumannii in traumatology department, so as to guide the rational use of antibiotics as well as prevent the nosocomial infection. Me thods A total of 96 strains of Acinetobacter baumannii were collected in traumatology department. Patient history were analyzed and genotype of carbapenemases of OXA-51, OXA-23, ISAba1-oxa-51 and ISAba1-oxa-23 were analyzed by PCR.Re s ults 70.84% (68/96) of Acinetobacter baumannii were isolated from wound tissue. 12 antibacterial agents were tested, 96 strains of Acinetobacter baumannii showed the highest resistance rate (78.13%) to imipenem and the lowest resistance rate (43.75%) to levofloxacin. Among 23 strains of imipenem-sensitive Acinetobacter baumannii, OXA-51 gene expression was not detected in 4 strains, however, OXA-23 gene expression were observed in 5 strains. The gene expression rates of OXA-51, OXA-23, ISAba1-oxa-51 and ISAba1-oxa-23 genes were 100%, 95.89%, 79.45% and 71.23% respectively in 73 strains of imipenem-insensitive Acinetobacter baumannii. The rate of gene expression profile of OXA-51+ISAba1-oxa-51+OXA-23+ISAba1-oxa-23 was 65.75%.Conclus ion The strains of imipenem-sensitive Acinetobacter baumannii may have the gene expression of OXA-23 of D class of carbapenemase.The main pattern of carbapenemase-producing was OXA-51+ISAba1-oxa-51+OXA-23+ISAba1-oxa-23 in traumatology department, which might be regarded as one of the mechanisms that led to the high resistance of the bacterium of acinetobacter baumannii to carbapenem antibiotics.
- Acinetobacter baumannii,
- Carbapenase,
- Genotype,
- Traumatology department

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References(12)

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