The Effect of Astragalus Polysaccharide on the Expressions of Nephrin and Desmin in High Sugar Stimulation Mice Podocytes

Huang Jie; Fan Wen Xing; Peng Qing Yan; Xiao Hua

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Article Navigation > Journal of Kunming Medical University > 2018 > 39(06): 26-30

Huang Jie , Fan Wen Xing , Peng Qing Yan , Xiao Hua . The Effect of Astragalus Polysaccharide on the Expressions of Nephrin and Desmin in High Sugar Stimulation Mice Podocytes[J]. Journal of Kunming Medical University, 2018, 39(06): 26-30.

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Huang Jie , Fan Wen Xing , Peng Qing Yan , Xiao Hua . The Effect of Astragalus Polysaccharide on the Expressions of Nephrin and Desmin in High Sugar Stimulation Mice Podocytes[J]. Journal of Kunming Medical University, 2018, 39(06): 26-30.

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The Effect of Astragalus Polysaccharide on the Expressions of Nephrin and Desmin in High Sugar Stimulation Mice Podocytes

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基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目[2017FE467 (-043) ];

Received Date: 2018-04-13

Abstract

Abstract

Objective To study the effect of Astragalus polysaccharide on the expressions of Nephrin and Desmin in high sugar stimulation mice podocytes. Me thods Test one: the cultured mouse podocytes were divided into 5 groups: astragalus polysaccharide group [D-glucose 30 mmol/L + astragalus polysaccharide (0.0 g/L, 0.1 g/L, 0.2 g/L, 0.4 g/L and 0.8 g/L) ]. Test two: In test one, we chose the concentration of astragalus polysaccharide which most affected both expression of Nephrin and Desmin in podocytes and named it as astragalus polysaccharide 0.X g/L. The cultured mouse podocytes were divided into 7 groups: astragalus polysaccharide group (D-glucose 30 mmol/L + astragalus polysaccharide 0.X g/L) had been cultured for 0 h, 3 h, 6 h, 12 h, 24 h, 48 h and 72 h, respectively. Test three: Basted on test two, we chose the time which most affected both expression of Nephrinand Desmin in podocytes and named it as Yh. The cultured mouse podocytes were divided into 4 groups: the control group, mannitol hypertonic glucose control group, high sugar group, and astragalus polysaccharide group (high sugar group + astragalus polysaccharide 0.X g/L) . Each group had been cultured for Yh. Nephrin and Desmin of each subgroup was detected by flow cytometry podocytes and real-time PCR. Re s ults With the intervention of polysaccharide, Nephrin in podocyte cell surface gradually increased, Desmin gradually reduced in a concentration-dependent manner ( P <0.05) . With the intervention of astragalus polysaccharide, Nephrin gradually increase and Desmin expression gradually decreased in a concentration-dependent manner (P<0.05) .Compared to the control group and the high sugar group, Nephrin mR NA increased and Desmin mR NA decreased in0.8 g/L astragalus polysaccharide co-cultured for 72 h ( P <0.05) . Conclus ion Astragalus polysaccharide intervention can increase Nephrin and reduce Desmin in podocytes, which are possible targets of Astragalus polysaccharide in treatment of DN proteinuria.
- Astragalus polysaccharide,
- Podocytes,
- Nephrin,
- Desmin

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References(15)

References

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