The Effect of Astragalus Polysaccharide on the Expressions of Nephrin and Desmin in High Sugar Stimulation Mice Podocytes

Huang Jie; Fan Wen Xing; Peng Qing Yan; Xiao Hua

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Article Navigation > Journal of Kunming Medical University > 2018 > 39(06): 26-30

Huang Jie , Fan Wen Xing , Peng Qing Yan , Xiao Hua . The Effect of Astragalus Polysaccharide on the Expressions of Nephrin and Desmin in High Sugar Stimulation Mice Podocytes[J]. Journal of Kunming Medical University, 2018, 39(06): 26-30.

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Huang Jie , Fan Wen Xing , Peng Qing Yan , Xiao Hua . The Effect of Astragalus Polysaccharide on the Expressions of Nephrin and Desmin in High Sugar Stimulation Mice Podocytes[J]. Journal of Kunming Medical University, 2018, 39(06): 26-30.

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The Effect of Astragalus Polysaccharide on the Expressions of Nephrin and Desmin in High Sugar Stimulation Mice Podocytes

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基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目[2017FE467 (-043) ];

Received Date: 2018-04-13

Abstract

Abstract

Objective To study the effect of Astragalus polysaccharide on the expressions of Nephrin and Desmin in high sugar stimulation mice podocytes. Me thods Test one: the cultured mouse podocytes were divided into 5 groups: astragalus polysaccharide group [D-glucose 30 mmol/L + astragalus polysaccharide (0.0 g/L, 0.1 g/L, 0.2 g/L, 0.4 g/L and 0.8 g/L) ]. Test two: In test one, we chose the concentration of astragalus polysaccharide which most affected both expression of Nephrin and Desmin in podocytes and named it as astragalus polysaccharide 0.X g/L. The cultured mouse podocytes were divided into 7 groups: astragalus polysaccharide group (D-glucose 30 mmol/L + astragalus polysaccharide 0.X g/L) had been cultured for 0 h, 3 h, 6 h, 12 h, 24 h, 48 h and 72 h, respectively. Test three: Basted on test two, we chose the time which most affected both expression of Nephrinand Desmin in podocytes and named it as Yh. The cultured mouse podocytes were divided into 4 groups: the control group, mannitol hypertonic glucose control group, high sugar group, and astragalus polysaccharide group (high sugar group + astragalus polysaccharide 0.X g/L) . Each group had been cultured for Yh. Nephrin and Desmin of each subgroup was detected by flow cytometry podocytes and real-time PCR. Re s ults With the intervention of polysaccharide, Nephrin in podocyte cell surface gradually increased, Desmin gradually reduced in a concentration-dependent manner ( P <0.05) . With the intervention of astragalus polysaccharide, Nephrin gradually increase and Desmin expression gradually decreased in a concentration-dependent manner (P<0.05) .Compared to the control group and the high sugar group, Nephrin mR NA increased and Desmin mR NA decreased in0.8 g/L astragalus polysaccharide co-cultured for 72 h ( P <0.05) . Conclus ion Astragalus polysaccharide intervention can increase Nephrin and reduce Desmin in podocytes, which are possible targets of Astragalus polysaccharide in treatment of DN proteinuria.
- Astragalus polysaccharide,
- Podocytes,
- Nephrin,
- Desmin

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References(15)

References

[1]	[1]LI M, WANG W, XUE J, et al.Meta-analysis of the clinical value of Astragalus membranaceus in diabetic nephropathy[J].J Ethnopharmacol, 2011, 133 (1) :412-419.
[2]	[2]BOSE M, ALMAS S, PRABHAKAR S.Wnt signaling and podocyte dysfunction in diabetic nephropathy[J].J Investig Med, 2017, 65 (8) :1093-1101.
[3]	[3] GUO C, LIU Y, ZHAO W, et al.Apelin promotes diabetic nephropathy by inducing podocyte dysfunction via inhibiting proteasome activities[J].J Cell Mol Med, 2015, 19 (9) :2273-2285.
[4]	[4]NAKHJAVANI M, AGHAJANI NARGESI A, SALABATI M, et al.Changes in Leukocyte Subpopulations with Decline in Glomerular Filtration Rate in Patients with Type 2Diabetes[J].Acta Med Iran, 2015, 53 (7) :425-431.
[5]	[5]JOURDAN T, SZANDA G, ROSENBERG A Z, et al.Overactive cannabinoid 1 receptor in podocytes drives type2 diabetic nephropathy[J].Proc Natl Acad Sci U S A, 2014, 111 (50) :E5420-E5428.
[6]	[6]DUMONT V, TOLVANEN TA, KUUSELA S, et al.PACSIN2 accelerates nephrin trafficking and is up-regulated in diabetic kidney disease[J].FASEB J, 2017, 31 (9) :3978-3990.
[7]李志杰, 张悦, 刘煜敏, 等.黄芪多糖对早期糖尿病肾病大鼠足细胞nephrin和podocin表达的影响[J].中国病理生理杂志, 2011, 27 (09) :1772-1776.
[8]	[8]HONG T, CUI LK, WEN J, et al.Cordycepin protects podocytes from injury mediated by complements complex C5b-9[J].Sichuan Da Xue Xue Bao Yi Xue Ban, 2015, 46 (2) :173-227.
[9]	[9]ZHAI L, GU J, YANG D, et al.Metformin ameliorates podocyte damage by restoring renal tissue nephrin expression in type 2 diabetic rats[J].J Diabetes, 2017, 9 (5) :510-517.
[10]	[10]GUO C, LIU Y, ZHAO W, et al.Apelin promotes diabetic nephropathy by inducing podocyte dysfunction via inhibiting proteasome activities[J].J Cell Mol Med, 2015, 19 (9) :2273-2285.
[11]	[11]ZHANG J, HU X, WANG S, et al.Protective effects of low-dose rapamycin combined with valsartan on podocytes of diabetic rats[J].Int J Clin Exp Med, 2015, 8 (8) :13275-13281.
[12]王竹, 刘俊田, 孙万森, 等.祛风通络方对阿霉素肾病大鼠足细胞Desmin及CD2AP蛋白的影响[J].中国中西医结合杂志, 2014, 34 (2) :203-208.
[13]张智颖.解毒通络保肾胶囊对实验性糖尿病肾病大鼠Desmin的实验研究[D].长春中医药大学, 2013.
[14]	[14]XIAO F, HU Y G, WU S N, et al.Protective effect of astragalus saponin extracts on kidneys of diabetic rats[J].Zhongguo Zhong Yao Za Zhi, 2015, 40 (10) :2014-8.
[15]	[15]ZHANG J, XIE X, LI C, et al.Systematic reviewof the renal protective effect of Astragalus membranaceus (root) on diabetic nephropathy in animal models[J].J Ethnopharmacol, 2009, 126 (2) :189-196.

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