A Study of LPS Mediated Inflammatory Oxidation Reaction of Three Types of Rodents

Chen Zhong Yi; Qiao Ting Ting; Dong Bao Lian; Guo Ling

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Chen Zhong Yi , Qiao Ting Ting , Dong Bao Lian , Guo Ling . A Study of LPS Mediated Inflammatory Oxidation Reaction of Three Types of Rodents[J]. Journal of Kunming Medical University, 2018, 39(06): 7-13.

Citation:

Chen Zhong Yi , Qiao Ting Ting , Dong Bao Lian , Guo Ling . A Study of LPS Mediated Inflammatory Oxidation Reaction of Three Types of Rodents[J]. Journal of Kunming Medical University, 2018, 39(06): 7-13.

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A Study of LPS Mediated Inflammatory Oxidation Reaction of Three Types of Rodents

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基金：国家自然科学基金资助项目 (81760228); 云南省科技计划基金资助项目 (2017FD193);

Received Date: 2017-12-17

Abstract

Abstract

Objective By comparing with the SD rats mixed glial cells, C57 mice mixed glial cells and Kunming mice mixed glial cells and exploring the expression of inflammatory factor of three mixed glial cells induced by lipopolysaccharide (LPS) , the study aimed to explore the application value of three kinds of mice and find the ideal model of inflammation. Me thods We used LPS as inducers, and NO, IL-1β, COX-2 and i NOS as anti-inflammatory antioxidant index. After cutting the head and taking the brain, we cultured the mixed glials. Then we used Greiss assay to detect the expression of NO and used western blot to detect the expression of protein of IL-1β, COX-2, and i NOS protein.Finally we compared the mixed glial cells from SD rats, C57 mice mixed glial cells and Kunming mouse mixed glial cells and selected the best inflammation model from three mixed glial cells.Re s ults The results showed that the morphological changes of the mixed glial cells in SD rats were treated with N2-free medium. Compared with the control group, the quantity of NO of LPS group of three mixed glial cells increased significantly (P<0.01) . The LPS group of SD rats released the highest concentration of NO. Western blot was used to detect the expression of IL-1β, Cox-2 and i NOS in three kinds of rodents. Compared with the blank control group, the expression of COX-2 protein, i NOS and IL-1β in the LPS group of the three mice increased significantly. The results showed that LPS could successfully stimulate the release of inflammatory cytokines in three kinds of mice, among which the SD rats were more sensitive and it could be used in the study of AD inflammation model. Conclus ion The results showed that LPS could induce the release of NO and the expression of IL-1β, i NOS and COX-2 in C57 BL/6 mice, Kunming mice and SD rats to induce inflammatory response. Thus, LPS can induce the formation of inflammatory oxidation models of the original mixed glial cells of the three mice. Moreover, the SD rats were more sensitive.
- LPS,
- Kunming Mouse,
- C57 Mouse,
- SD rats,
- NO,
- Interleukin-1β

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References(16)

References

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