Effects of Rutin on Proliferation of Keloid Fibroblasts

Li Yu Peng; Jiao Jian Lin; Lv Le Chun

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Li Yu Peng , Jiao Jian Lin , Lv Le Chun . Effects of Rutin on Proliferation of Keloid Fibroblasts[J]. Journal of Kunming Medical University, 2018, 39(07): 1-4.

Citation:

Li Yu Peng , Jiao Jian Lin , Lv Le Chun . Effects of Rutin on Proliferation of Keloid Fibroblasts[J]. Journal of Kunming Medical University, 2018, 39(07): 1-4.

Li Yu Peng , Jiao Jian Lin , Lv Le Chun . Effects of Rutin on Proliferation of Keloid Fibroblasts[J]. Journal of Kunming Medical University, 2018, 39(07): 1-4.

Citation:

Li Yu Peng , Jiao Jian Lin , Lv Le Chun . Effects of Rutin on Proliferation of Keloid Fibroblasts[J]. Journal of Kunming Medical University, 2018, 39(07): 1-4.

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Effects of Rutin on Proliferation of Keloid Fibroblasts

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基金：国家自然科学基金资助项目 (81560502); 云南省自然科学基金资助项目 (2017FB116); 昆明医科大学“百名中青年学术和技术骨干”资助项目;

Received Date: 2018-02-13

Abstract

Abstract

Objective To investigate the effects of rutin on the proliferation of keloid fibroblasts.Methods Keloid tissue was used to induce fibroblasts and 5 concentration gradients of rutin were set: 0, 50, 100, 200 and300 μmol/L.The effects of rutin on the proliferation of keloid-derived fibroblasts were observed by CCK-8 and Ed U assays.Western blot was applied to detect the effect of rutin on TGF-β/Smad signaling pathway.Re s ults Rutin inhibited the proliferation of keloid-derived fibroblasts in a dose-dependent manner when concentration gradient was lower than 300 μmol/L (P<0.01) . The result of Ed U assay showed that the synthesis of DNA in keloid-derived fibroblasts was inhibited by rutin. At the concentration gradient of 50, 100 and 200 μmol/L, rutin could significantly inhibit the expression of TGF-β1 and Smad2 in keloid-derived fibroblasts.Conclus ion Rutin inhibits the proliferation of keloid-derived fibroblasts by regulating TGF-β/Smad signaling pathway.
- Keloid,
- Fibroblast,
- Rutin,
- Proliferation

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References(8)

References

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