Effect of Ginsenoside Rg3 on Mouse Neural Stem Cells Proliferation in Vitro

Zhang Feng Lan; Zhao Yu; Yuan Jing; Yang Lu Jun; Lu Lin

Article Contents

Article Navigation > Journal of Kunming Medical University > 2018 > 39(07): 10-14

Zhang Feng Lan , Zhao Yu , Yuan Jing , Yang Lu Jun , Lu Lin . Effect of Ginsenoside Rg3 on Mouse Neural Stem Cells Proliferation in Vitro[J]. Journal of Kunming Medical University, 2018, 39(07): 10-14.

Citation:

Zhang Feng Lan , Zhao Yu , Yuan Jing , Yang Lu Jun , Lu Lin . Effect of Ginsenoside Rg3 on Mouse Neural Stem Cells Proliferation in Vitro[J]. Journal of Kunming Medical University, 2018, 39(07): 10-14.

Citation:

PDF( 1986 KB)

Effect of Ginsenoside Rg3 on Mouse Neural Stem Cells Proliferation in Vitro

Zhang Feng Lan ¹,
Zhao Yu ²,
Yuan Jing ^1,3,
Yang Lu Jun ⁴,
Lu Lin ^1,5

Funds:

基金：云南省教育厅科学研究基金资助项目 (2014C039Y, 2016ZDX041);

Received Date: 2018-02-24

Abstract

Abstract

Objective To investigate the effect of ginsenoside Rg3 on mouse neural stem cells (NSCs) proliferation in vitro.Me thods Fetal cortices of embryonic 14 d (E14) C57 BL/6 mouse were isolated for culturing primary NSCs.After being passaged twice, NSCs specific antibodies, Nestin and Sox2 were identified by immunocyto chemistry staining. CCK-8 assay and Brd U labelling assay were used to measure NSCs proliferation in response to different ginsenoside Rg3 concentrations (20 n M, 50 n M, 250 n M and 500 n M) . Re s ults Primary and passaged NSCs were successfully cultured and almost all the cells were both Nestin and Sox2 positive NSCs, which indicated high purity of NSCs. Brd U labelling assay showed the group of 50 n M Rg3 had the highest relative ratio of Brd U positive percentage, and the difference with other groups was statistically significant (P<0.01) .CCK-8 assay also showed 50 n M Rg3 group had the highest OD value, which was significantly higher than the control group (P<0.01) , 20 n M group (P<0.05) and 500 n M group (P<0.01) , while other groups had no significant difference between each other (P >0.05) . Conclus ion 50 n M ginsenoside Rg3 could significantly promote mouse NSCs proliferation in vitro.
- Neural stem cells,
- Ginsenoside Rg3,
- Proliferation,
- BrdU,
- CCK-8

FullText(HTML)

References(16)

References

[1]	[1]TORRADO E F, GOMES C, SANTOS G, et al.Directing mouse embryonic neurosphere differentiation toward an enriched neuronal population[J].Int J Dev Neurosci, 2014, 37 (2014) :94-99.
[2]	[2]WALKER T, HUANG J, YOUNG K.Neural stem and progenitor cells in nervous system function and therapy[J].Stem Cells Int, 2016, 6 (2016) :1890568.
[3]	[3]LEE R, KIM IS, HAN N, et al.Real-time discrimination between proliferation and neuronal and astroglial differentiation of human neural stem cells[J].Sci Rep, 2014, 9 (4) :6319.
[4]石海杉, 吴文, 徐建兰.内源性神经干细胞的研究进展[J].实用医学杂志, 2013, 29 (19) :3252-3254.
[5]赵瑜, 袁婧, 鲁琳.小鼠胚胎脑源与脊髓源神经干细胞增殖能力的比较[J].昆明医科大学学报, 2017, 38 (9) :19-23.
[6]胡炜彦, 于浩飞, 张荣平.人参皂苷Rg3对H2O2导致海马神经元损伤的保护作用研究[J].中成药, 2014, 36 (4) :670-674.
[7]	[7]KIM H S, LEE E H, KOSR, et al.Effects of ginsenosides rg3 and rh2 on the proliferation of prostate cancer cells[J].Arch Pharm Res, 2004, 27 (4) :429-435.
[8]孙建忠, 刘新伟, 管华鹏, 等.人参皂苷Rg1与神经干细胞共培养的促增殖和保护作用[J].中国组织工程研究, 2015, 19 (10) :1580-1584.
[9]郑玉芹, 姜正林, 徐美玉, 人参皂苷Rb1对体外培养胎鼠神经干细胞增殖及分化的影响[J].神经解剖学杂志, 2014, 30 (3) :273-279.
[10]	[10]BABU H, CLAASEN J H, KANNAN S, et al.A protocol for isolation and enriched monolayer cultivation of neural precursor cells from mouse dentate gyrus[J].Front Neurosci, 2011, 5 (89) :1-10.
[11]	[11]XIONG F, GAO H, ZHEN Y, et al.Optimal time for passaging neurospheres based on primary neural stem cell cultures[J].Cytotechnology, 2011, 63 (6) :621-631.
[12]	[12]SINGEC I, KNOTH R, MEYER RP, et al.Defining the actual sensitivity and specificity of the neurosphere assay in stem cell biology[J].Nat Methods, 2006, 3 (10) :801-806.
[13]	[13]ZHANG J, JIAO JW.Molecular Biomarkers for Embryonic and Adult Neural Stem Cell and Neurogenesis[J].Biomed Res Int, 2015, 2015:1-14.
[14]	[14]XU R, WU C, TAO Y, et al.Description of distributed features of the nestin-containing cells in brains of adult mice:a potential source of neural precursor cells[J].J Neurosci Res, 2010, 88 (5) :945-956.
[15]	[15]ZHANG S, CUI W.Sox2, a key factor in the regulation of pluripotency and neural differentiation[J].World J Stem Cells, 2014, 6 (3) :305-311.
[16]	[16]MORTE M I, CARREIRA B P, MACHADO V, et al.Evaluation of proliferation of neural stem cells in vitro and in vivo[J].Curr Protoc Stem Cell Biol, 2013, 2 (14) :1-14.

Relative Articles(20)

Relative Articles

[1]	Ling WANG, Xiangchuan QIN, Jinqiu LI, Hasim AXIANGU. CD147 Mediates Cervical Cancer Cell Pyroptosis and Proliferation through AIM2 Inflammasome. Journal of Kunming Medical University, 2024, 45(1): 15-21. doi: 10.12259/j.issn.2095-610X.S20240103
[2]	Yelin ZHANG, Liya MA, Xuhui PENG, Hefeng YANG, Rui SHE. Hsa-let-7a-5p Regulates Proliferation and Apoptosis of Periodontal Ligament Stem Cells. Journal of Kunming Medical University, 2023, 44(10): 47-54. doi: 10.12259/j.issn.2095-610X.S20231028
[3]	Liang ZHANG, Baoquan WANG, Xifeng LEI, Xu WANG, Yang KE, Wei ZHANG. Effect of miR-29c-3p/IGF1 Molecular Axis on Activation，Proliferation and Apoptosis of Hepatic Stellate Cells. Journal of Kunming Medical University, 2023, 44(9): 7-14. doi: 10.12259/j.issn.2095-610X.S20230926
[4]	Baoquan WANG, Wei ZHANG, Yuan TIAN, Xifeng LEI, Xu WANG. miR-142-5p Regulates the Proliferation and Metastasis of Gallbladder Carcinoma Cells through CCND1. Journal of Kunming Medical University, 2022, 43(2): 47-53. doi: 10.12259/j.issn.2095-610X.S20220223
[5]	Wei ZHANG, Baoquan WANG, Xifeng LEI, Xu WANG, Liang ZHANG. miR-125b-5p Regulates HK2 to Inhibit Proliferation and Glycolysis of Gallbladder Cancer Cells. Journal of Kunming Medical University, 2022, 43(12): 23-29. doi: 10.12259/j.issn.2095-610X.S20221206
[6]	Zhoujun LIAO, Shaohua YANG, Lixin LIU, Sheng HU, Yihui CHEN, Qiang KANG, Xiaowen ZHANG. Effect of AK4 on Proliferation and Migration of Intrahepatic Bile Duct Carcinoma Cell HUCCT1. Journal of Kunming Medical University, 2022, 43(6): 1-6. doi: 10.12259/j.issn.2095-610X.S20220611
[7]	Lan YANG, Xiao JIA, Yi-tong JIANG, Qi CUI, Guang-ci LIU, Ying-hong HE. The Effect of Silencing of UBE2C Gene on the Proliferation and Migration of Human Gastric Cancer AGS Cells. Journal of Kunming Medical University, 2021, 42(5): 18-23. doi: 10.12259/j.issn.2095-610X.S20210504
[8]	Zhao Yu , Yuan Jing , Liu Jian Jun , Lu Lin . Comparison of Proliferation between Fetal Forebrain-and Spinal Cord-Derived Neural Stem Cells in Mice. Journal of Kunming Medical University, 2017, 38(09): 19-23.
[9]	Wei Dong . Effect of Fragile Site WWOX Gene on Regulating Proliferation of Human Gallbladder Cancer Cells in Vitro. Journal of Kunming Medical University,
[10]	Jia Nan Nan . Effect of Gnaq on the Proliferation of SH-SY5Y Cells and Its Mechanism of Action. Journal of Kunming Medical University,
[11]	Huang Yun Yan . . Journal of Kunming Medical University,
[12]	Zhao Yu Xin . . Journal of Kunming Medical University,
[13]	Zeng Jin . . Journal of Kunming Medical University,
[14]	Xia Ying Jie . . Journal of Kunming Medical University,
[15]	Zeng Jin . 人参皂苷Rg3、干扰素α联合与单一用药治疗血吸虫病肝纤维化疗效对比. Journal of Kunming Medical University,
[16]	Wu Bin . . Journal of Kunming Medical University,
[17]	. Growth Characteristics of Lung Adenocarcinoma Cell Lines A549 and Brdu Expression in Vitro. Journal of Kunming Medical University,
[18]	Li Yu . . Journal of Kunming Medical University, 2011, 32(02): 1-1.
[19]	Yang Jin Wei . . Journal of Kunming Medical University,
[20]	Li Xiong . . Journal of Kunming Medical University,