Effects of Methamphetamine on the Expression of 5-HT, IL-6 and NF-κB in Rats' Hippocampus

Yang Jin Feng; Meng Guo Yi; Huang Zhao Kui; Hong Shi Jun; Li Li Hua; Xing Yu Ming; Zhao Yong Na

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Yang Jin Feng , Meng Guo Yi , Huang Zhao Kui , Hong Shi Jun , Li Li Hua , Xing Yu Ming , Zhao Yong Na . Effects of Methamphetamine on the Expression of 5-HT, IL-6 and NF-κB in Rats' Hippocampus[J]. Journal of Kunming Medical University, 2018, 39(07): 5-9.

Citation:

Yang Jin Feng , Meng Guo Yi , Huang Zhao Kui , Hong Shi Jun , Li Li Hua , Xing Yu Ming , Zhao Yong Na . Effects of Methamphetamine on the Expression of 5-HT, IL-6 and NF-κB in Rats' Hippocampus[J]. Journal of Kunming Medical University, 2018, 39(07): 5-9.

Citation:

Yang Jin Feng , Meng Guo Yi , Huang Zhao Kui , Hong Shi Jun , Li Li Hua , Xing Yu Ming , Zhao Yong Na . Effects of Methamphetamine on the Expression of 5-HT, IL-6 and NF-κB in Rats' Hippocampus[J]. Journal of Kunming Medical University, 2018, 39(07): 5-9.

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Effects of Methamphetamine on the Expression of 5-HT, IL-6 and NF-κB in Rats' Hippocampus

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基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2014FB018);

Received Date: 2018-02-12

Abstract

Abstract

Objective To study the effects of methamphetamine on the expression of 5-Hydroxytryptophan (5-HT) , interleukin-6 (IL-6) and nuclear fact kappa B (NF-κB) in rats' hippocampus. Methods Thirty SD rats were randomly allocated into normal control group, METH1 d group, METH3 d group, METH5 d group and METH7 d group, with 6 rats in each group.Intraperitoneal injections of METH [15 mg/ (kg·time) ] were conducted at9:00 am and 21:00 pm every day.After injection, scoring was performed in combination with observation on their stereotyped behavior. We established different models for rats suffering from 1-day, 3-day, 5-day, and 7-day METH acute poisoning. After 24 hours of the last intraperitoneal injections of METH, hippocampal tissues were isolated.Enzyme linked immunoorbent assay (ELISA) was applied to detect the expression level of 5-HT and IL-6 in rats hippocampus. Western blot was used to detect the expression level of NF-κBp65.Re s ults Compared with the control group, the expression of 5-HT was reduced in different METH groups, and the decrease was statistically significant in METH5 d and METH7 d groups (with P<0.05 and P<0.01 respectively) . Compared with the control group, the expression of IL-6 was increased in different METH groups, and the increase was statistically significant in METH5 d and METH7 d groups (with P<0.05 and P<0.01 respectively) . Compared with the control group, the expression of NF-κB was increased in different groups, and the increase was statistically significant only in METH7 d group (P <0.01) . Conclus ion METH administration could result in a significant reduce of 5-HT expression and increases of IL-6 and NF-κB expressions in rats hippocampus.
- Methamphetamine,
- Hippocampal tissue,
- 5-HT,
- IL-6,
- NF-κB

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References(18)

References

[1]李学峰, 丘平明, 等.甲基苯丙胺对相关脑区的神经毒性作用[J].解剖学杂志, 2007, 30 (1) :63-65.
[2]	[2]JOHN D, ROBINSON, CHRISTOPHER D, et al.Methamphetamine-induced neurotoxicity disrupts pharmacologically evoked dopamine transients in the dorsomedial and dorsolateral striatum[J].Neurotox Res, 2014, 26 (2) 152-167.
[3]	[3]AMANDA SIERRA, ASTRID E, CARDONA, et al.Functional diversity of microglia-how heterogenenous are they to begin with[J].Frontiers in Cellular Neuroscience, 2013, 14 (5) :1-18.
[4]	[4]BALDWIN A S.The NF-κB and IκB proteins:new discoveries and insights[J].Annu Rev Immunol, 1996, 14 (1) :649-683.
[5]	[5]KARIN M, BEN-NERIAH Y.Phosphorylation meets ubiquitination the control of NF-κB activity[J].Annu Rev Immunol, 2000, 18 (1) :621-663.
[6]	[6]RYANT, MOTT, MARTIANVENDRAME, et al.Neuronal expression of CD22:Novel nechanism for inhibiting microglial Proinflammatory cytokine production[J].GLIA, 2004, 46 (4) :369-379.
[7]	[7]SAMS-DODD F.Effects of continuous D-amphetamine and phencyclidine administration onsocial behaviour, stereotyped behaviour, and locomotor activity in rats[J].Neuropsychopha Rmacology, 1998, 19 (1) :18-25.
[8]	[8]THOMAS D M, DOWGIERT J, GEDDES T J, et al.Microglial activation is a pharmacologically specific marker for the neurotoxic amphetamines[J].Neurosci Lett, 2004, 367 (3) :349-354.
[9]	[9]GONCALVES J, RIBEIRO C F, MALVA J O, et al.Protective role of neuropeptide Y Y (2) receptors in cell death and microglial response following methamphetamine injury[J].Eur J Neurosci, 2012, 36 (9) :3173-3183.
[10]	[10]AIZENMAN E, MCCORD M C, SAADI R A, et al.Complex role of zinc in methamphetamine toxicity in vitro[J].Neuroscience, 2010, 171 (1) :31-39.
[11]	[11]MCCANN U D, KUWABARA H, KUMAR A, et al.Persistent cognitive and dopamine transporter deficits in abstinent methamphetamine users[J].Synapse, 2008, 62 (2) :91-100.
[12]苟红艳.CCK-8对甲基苯丙胺诱导神经损伤的影响及其抗炎机制研究[D].石家庄:河北医科大学, 2015.
[13]	[13]GONCALVES J, MARITINS T, MILHAZES N, et al.Methamphetmine-induced neuroinflammation of neuronal dysfunction in the mice hippocampus:preventive effect of indomethacin[J].Eur J Neurosci, 2010, 31 (2) :315-326.
[14]	[14]LEE Y W, HENNING B, YAO J, et al.Methamphetamine induces AP-1 and NF-kappa B binding and transanctivation in human brain endothedial cell[J].J Neurosci Res, 2001, 66 (4) :583-591.
[15]	[15]SHIFERA A S.The zinc finger domain of ikkgamma (Nemo) protein in health and disease[J].Cell Mol Med, 2010, 14 (10) :2404-2414.
[16]	[16]BAKER R G, HAYDEN M S, GHOSH S.NF-κB, Inflammation, and metabolic disease[J].Cell Metabolism, 2011, 13 (4) :11-22.
[17]	[17] MOTT R T, AIT-GHEZALA G, TOWN T, et al.Neuronal expression of CD22:novel mechanism for inhibiting microglial proinflammatory cytokine production.[J].Glia, 2004, 46 (4) :369-379.
[18]	[18]NAGATSU T.Parkinson.5 disease:changes in apoptosis-related faetors suggesting Possible gene therapy[J].Neural Transm, 2002, 109 (526) :731-745.

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