Effects of Methamphetamine on the Expression of 5-HT, IL-6 and NF-κB in Rats' Hippocampus

Yang Jin Feng; Meng Guo Yi; Huang Zhao Kui; Hong Shi Jun; Li Li Hua; Xing Yu Ming; Zhao Yong Na

Article Contents

Article Navigation > Journal of Kunming Medical University > 2018 > 39(07): 5-9

Yang Jin Feng , Meng Guo Yi , Huang Zhao Kui , Hong Shi Jun , Li Li Hua , Xing Yu Ming , Zhao Yong Na . Effects of Methamphetamine on the Expression of 5-HT, IL-6 and NF-κB in Rats' Hippocampus[J]. Journal of Kunming Medical University, 2018, 39(07): 5-9.

Citation:

Yang Jin Feng , Meng Guo Yi , Huang Zhao Kui , Hong Shi Jun , Li Li Hua , Xing Yu Ming , Zhao Yong Na . Effects of Methamphetamine on the Expression of 5-HT, IL-6 and NF-κB in Rats' Hippocampus[J]. Journal of Kunming Medical University, 2018, 39(07): 5-9.

Citation:

Yang Jin Feng , Meng Guo Yi , Huang Zhao Kui , Hong Shi Jun , Li Li Hua , Xing Yu Ming , Zhao Yong Na . Effects of Methamphetamine on the Expression of 5-HT, IL-6 and NF-κB in Rats' Hippocampus[J]. Journal of Kunming Medical University, 2018, 39(07): 5-9.

PDF( 487 KB)

Effects of Methamphetamine on the Expression of 5-HT, IL-6 and NF-κB in Rats' Hippocampus

Funds:

基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2014FB018);

Received Date: 2018-02-12

Abstract

Abstract

Objective To study the effects of methamphetamine on the expression of 5-Hydroxytryptophan (5-HT) , interleukin-6 (IL-6) and nuclear fact kappa B (NF-κB) in rats' hippocampus. Methods Thirty SD rats were randomly allocated into normal control group, METH1 d group, METH3 d group, METH5 d group and METH7 d group, with 6 rats in each group.Intraperitoneal injections of METH [15 mg/ (kg·time) ] were conducted at9:00 am and 21:00 pm every day.After injection, scoring was performed in combination with observation on their stereotyped behavior. We established different models for rats suffering from 1-day, 3-day, 5-day, and 7-day METH acute poisoning. After 24 hours of the last intraperitoneal injections of METH, hippocampal tissues were isolated.Enzyme linked immunoorbent assay (ELISA) was applied to detect the expression level of 5-HT and IL-6 in rats hippocampus. Western blot was used to detect the expression level of NF-κBp65.Re s ults Compared with the control group, the expression of 5-HT was reduced in different METH groups, and the decrease was statistically significant in METH5 d and METH7 d groups (with P<0.05 and P<0.01 respectively) . Compared with the control group, the expression of IL-6 was increased in different METH groups, and the increase was statistically significant in METH5 d and METH7 d groups (with P<0.05 and P<0.01 respectively) . Compared with the control group, the expression of NF-κB was increased in different groups, and the increase was statistically significant only in METH7 d group (P <0.01) . Conclus ion METH administration could result in a significant reduce of 5-HT expression and increases of IL-6 and NF-κB expressions in rats hippocampus.
- Methamphetamine,
- Hippocampal tissue,
- 5-HT,
- IL-6,
- NF-κB

FullText(HTML)

References(18)

References

[1]李学峰, 丘平明, 等.甲基苯丙胺对相关脑区的神经毒性作用[J].解剖学杂志, 2007, 30 (1) :63-65.
[2]	[2]JOHN D, ROBINSON, CHRISTOPHER D, et al.Methamphetamine-induced neurotoxicity disrupts pharmacologically evoked dopamine transients in the dorsomedial and dorsolateral striatum[J].Neurotox Res, 2014, 26 (2) 152-167.
[3]	[3]AMANDA SIERRA, ASTRID E, CARDONA, et al.Functional diversity of microglia-how heterogenenous are they to begin with[J].Frontiers in Cellular Neuroscience, 2013, 14 (5) :1-18.
[4]	[4]BALDWIN A S.The NF-κB and IκB proteins:new discoveries and insights[J].Annu Rev Immunol, 1996, 14 (1) :649-683.
[5]	[5]KARIN M, BEN-NERIAH Y.Phosphorylation meets ubiquitination the control of NF-κB activity[J].Annu Rev Immunol, 2000, 18 (1) :621-663.
[6]	[6]RYANT, MOTT, MARTIANVENDRAME, et al.Neuronal expression of CD22:Novel nechanism for inhibiting microglial Proinflammatory cytokine production[J].GLIA, 2004, 46 (4) :369-379.
[7]	[7]SAMS-DODD F.Effects of continuous D-amphetamine and phencyclidine administration onsocial behaviour, stereotyped behaviour, and locomotor activity in rats[J].Neuropsychopha Rmacology, 1998, 19 (1) :18-25.
[8]	[8]THOMAS D M, DOWGIERT J, GEDDES T J, et al.Microglial activation is a pharmacologically specific marker for the neurotoxic amphetamines[J].Neurosci Lett, 2004, 367 (3) :349-354.
[9]	[9]GONCALVES J, RIBEIRO C F, MALVA J O, et al.Protective role of neuropeptide Y Y (2) receptors in cell death and microglial response following methamphetamine injury[J].Eur J Neurosci, 2012, 36 (9) :3173-3183.
[10]	[10]AIZENMAN E, MCCORD M C, SAADI R A, et al.Complex role of zinc in methamphetamine toxicity in vitro[J].Neuroscience, 2010, 171 (1) :31-39.
[11]	[11]MCCANN U D, KUWABARA H, KUMAR A, et al.Persistent cognitive and dopamine transporter deficits in abstinent methamphetamine users[J].Synapse, 2008, 62 (2) :91-100.
[12]苟红艳.CCK-8对甲基苯丙胺诱导神经损伤的影响及其抗炎机制研究[D].石家庄:河北医科大学, 2015.
[13]	[13]GONCALVES J, MARITINS T, MILHAZES N, et al.Methamphetmine-induced neuroinflammation of neuronal dysfunction in the mice hippocampus:preventive effect of indomethacin[J].Eur J Neurosci, 2010, 31 (2) :315-326.
[14]	[14]LEE Y W, HENNING B, YAO J, et al.Methamphetamine induces AP-1 and NF-kappa B binding and transanctivation in human brain endothedial cell[J].J Neurosci Res, 2001, 66 (4) :583-591.
[15]	[15]SHIFERA A S.The zinc finger domain of ikkgamma (Nemo) protein in health and disease[J].Cell Mol Med, 2010, 14 (10) :2404-2414.
[16]	[16]BAKER R G, HAYDEN M S, GHOSH S.NF-κB, Inflammation, and metabolic disease[J].Cell Metabolism, 2011, 13 (4) :11-22.
[17]	[17] MOTT R T, AIT-GHEZALA G, TOWN T, et al.Neuronal expression of CD22:novel mechanism for inhibiting microglial proinflammatory cytokine production.[J].Glia, 2004, 46 (4) :369-379.
[18]	[18]NAGATSU T.Parkinson.5 disease:changes in apoptosis-related faetors suggesting Possible gene therapy[J].Neural Transm, 2002, 109 (526) :731-745.

Relative Articles(20)

Relative Articles

[1]	Xiaoqing ZHAO, Jiaxin XIAO, Yong LIN, Jianfeng ZHANG, Wenhui WANG, Wenjie ZHANG. Effects of Different Virulence Types of Helicobacter Pylori on Gastric Micro-environment. Journal of Kunming Medical University, 2024, 45(2): 148-152. doi: 10.12259/j.issn.2095-610X.S20240221
[2]	Aheng WANG, Zhen SONG, Guanghua YANG, Haiyan HE. Regulatory Mechanisms of TLR4/NF-κB/ MUC5B in Allergic Rhinitis. Journal of Kunming Medical University, 2024, 45(11): 46-51. doi: 10.12259/j.issn.2095-610X.S20241107
[3]	Lin XU, Kaicheng GAO, Jie JIA, Yuyang LI, Huawei WANG, Yiqun KUANG, Yu ZHAO. Effect of Shen-ling-bai-zhu-san on Methamphetamine-Induced Gut Microbiota Change in Mice. Journal of Kunming Medical University, 2023, 44(8): 37-43. doi: 10.12259/j.issn.2095-610X.S20230829
[4]	Ranxi SHI, Tao YANG, Qing CHENG, Liangchen ZHAO, Jiahui GUO, Limei WANG. Mechanism of Dendrobium Officinale Against Inflammatory Aging Based on Network Pharmacology and Molecular Docking. Journal of Kunming Medical University, 2023, 44(11): 1-8. doi: 10.12259/j.issn.2095-610X.S20231101
[5]	Wenbiao SHI, Cailiang MA, Yalin ZHAO, Feng WANG, Julin WANG, Mihui ZHANG. Changes and Meaning of Serum IL-1β，IL-2，IL-4，IL-5， IL-6 in Patients with Pregnancy Syndrome in Hypertensive Diseases. Journal of Kunming Medical University, 2023, 44(9): 100-103. doi: 10.12259/j.issn.2095-610X.S20230909
[6]	Junhong MAO, Danfeng LU, Yu XU, Yicong DAI, Zherui ZHANG, Yue LI, Kunhua WANG. Methamphetamine Alters Methylation Modifications of Synaptic Plasticity Genes in Addicted Mice. Journal of Kunming Medical University, 2022, 43(1): 1-7. doi: 10.12259/j.issn.2095-610X.S20220121
[7]	Yuan-yuan LI, Juan LI, Gen-meng YANG, Jian HUANG, Liu LIU, Bao-yu SHEN, Chan WANG, Yue XU, Shu-cheng LIN, Xiao-feng ZENG. Research Progress on Neurotoxic Effects and Mechanism of Methamphetamine. Journal of Kunming Medical University, 2021, 42(2): 153-157. doi: 10.12259/j.issn.2095-610X.S20210210
[8]	Zhi-qiang LI, Hong-qiang GAO, Jing-feng LI, Jie-qi YU, Dong-dong WANG, Gang CHEN, Guang CHU. Expression of ICAM-1, MMP-9 and NF-κB in Cholangiocarcinoma and Their Relationship with Immunosuppressive Cells in Peripheral Blood. Journal of Kunming Medical University, 2021, 42(11): 93-98. doi: 10.12259/j.issn.2095-610X.S20211117
[9]	Ting-na ZHU, Peng-liang LIU, Wen-juan DONG, Hao YU, Ya-mei WU, Zhao-kui HUANG, Shi-jun HONG, Yong-na ZHAO. Effects of Different Doses of Gastrodin on Conditioned Place Preference and Microglia Activation in Hippocampus of Methamphetamine Dependent Rats. Journal of Kunming Medical University, 2021, 42(5): 6-11. doi: 10.12259/j.issn.2095-610X.S20210502
[10]	Wang Yi Hang , Hong Shi Jun , Zhong Lei Lei , Jie Run Fang , Peng Yan Xia , Li Li Hua . . Journal of Kunming Medical University, 2020, 41(03): 17-21.
[11]	Ma Chen Li , Yang Gen Meng , Yan Qing Wen , Zhang Feng Yi , Shi Zhen Jin , Zhou Yi Qing , Wang Yi Hang , Zhao Yong Na , Zhang Dong Xian , Li Li Hua , Hong Shi Jun . Expression of Astrocyte, Neuron, and Proinflammatory Cytokines Derived from Frontal Cortex of Methamphetamine Dependent rats after Gastrodin Administration. Journal of Kunming Medical University, 2018, 39(06): 1-6.
[12]	Tang Wei Wei , He Yong Wen . . Journal of Kunming Medical University, 2018, 39(10): 42-45.
[13]	Li Yan Ming , Zheng Yan Zhen , Zhang Dong Xian , Li Juan , Yang Gen Meng , He Yong Wang , Zhou Yi Qing , Zeng Xiao Feng , Li Zhen . Expression of NMDA Receptor Subunit 2B and iNOS in Rat Striatum by Synergizing Methamphetamine and Human Immunodeficiency Virus Protein Tat. Journal of Kunming Medical University, 2017, 38(07): 1-6.
[14]	Zhang Jin Pei , Yang Yi , Jin Yan . Effect of Ma Xiong Shu Tong Fang on Migraine and Its Influence on Serum Levels of 5-HT, NF-NF-κB and Inflamatory Cytokines. Journal of Kunming Medical University, 2017, 38(08): 40-43.
[15]	Meng Guo Yi , Sui Nian Han , Yang Jin Feng , Bian Li Gong , Hong Shi Jun , Li Li Hua , Zhao Yong Na . . Journal of Kunming Medical University, 2017, 38(02): 19-22.
[16]	Yang Jin Feng , Meng Guo Yi , Sui Nian Han , Bian Li Gong , Hong Shi Jun , Li Li Hua , Zhao Yong Na . Effects of Methamphetamine on the Expression of DA, 5-HT and TNF-α in Rats Hippocampus. Journal of Kunming Medical University, 2017, 38(10): 6-10.
[17]	Jia Nan Nan . Effect of Gnaq on the Proliferation of SH-SY5Y Cells and Its Mechanism of Action. Journal of Kunming Medical University,
[18]	Zeng Bai Rui . . Journal of Kunming Medical University,
[19]	Li Hong Li . . Journal of Kunming Medical University,
[20]	Ma Shan . Effects of Liangxuehuoxue Drugs on Hyperoxia Induced Retinal Neovascular HIF-1 and NF-кB Expression in Mice. Journal of Kunming Medical University, 2012, 33(10): 1-1.