Effects of CNTF Antibody Block to the Bone Marrow Mesenchymal Stem Cells Transplantated by Abdominal Aortic on the Downstream Signaling Pathways STAT3/Caspase-9 in Spinal Cord Ischemic Reperfusion Injuried Rats

Chen Shan Shan; Yi Min Chun; Zhou Guo Zhong; Pu Yue Chang; Hu Yi; Han Mi Hua; Jin Hua

Article Contents

Article Navigation > Journal of Kunming Medical University > 2018 > 39(08): 5-12

Chen Shan Shan , Yi Min Chun , Zhou Guo Zhong , Pu Yue Chang , Hu Yi , Han Mi Hua , Jin Hua . Effects of CNTF Antibody Block to the Bone Marrow Mesenchymal Stem Cells Transplantated by Abdominal Aortic on the Downstream Signaling Pathways STAT3/Caspase-9 in Spinal Cord Ischemic Reperfusion Injuried Rats[J]. Journal of Kunming Medical University, 2018, 39(08): 5-12.

Citation:

Chen Shan Shan , Yi Min Chun , Zhou Guo Zhong , Pu Yue Chang , Hu Yi , Han Mi Hua , Jin Hua . Effects of CNTF Antibody Block to the Bone Marrow Mesenchymal Stem Cells Transplantated by Abdominal Aortic on the Downstream Signaling Pathways STAT3/Caspase-9 in Spinal Cord Ischemic Reperfusion Injuried Rats[J]. Journal of Kunming Medical University, 2018, 39(08): 5-12.

Citation:

Chen Shan Shan , Yi Min Chun , Zhou Guo Zhong , Pu Yue Chang , Hu Yi , Han Mi Hua , Jin Hua . Effects of CNTF Antibody Block to the Bone Marrow Mesenchymal Stem Cells Transplantated by Abdominal Aortic on the Downstream Signaling Pathways STAT3/Caspase-9 in Spinal Cord Ischemic Reperfusion Injuried Rats[J]. Journal of Kunming Medical University, 2018, 39(08): 5-12.

PDF( 2340 KB)

Effects of CNTF Antibody Block to the Bone Marrow Mesenchymal Stem Cells Transplantated by Abdominal Aortic on the Downstream Signaling Pathways STAT3/Caspase-9 in Spinal Cord Ischemic Reperfusion Injuried Rats

Funds:

基金：国家自然科学基金资助项目 (31360246, 81300973); 云南省教育厅科学研究基金资助项目 (2016ZZX279); 云南省学术技术后备人才基金资助项目 (2017HB042); 云南省学术带头人基金资助项目 (D-201631);

Received Date: 2018-05-14

Abstract

Abstract

Objective To investigate the functional recovery and the effect of CNTF antibody block to the bone marrow mesenchymal stem cells (BMSCs) transplantated by Abdominal aortic on the downstream signaling pathways STAT3/Caspase-9 in spinal cord ischemic reperfusion injury in rats.Methods Adult female SD ratswere assigned randomly to 4 groups. The neurological functional status of the animals was assessed with BBB scores, the Motor evoked potentials (MEP) and Cortical somatosensory evoked potentials (CSEP) .The IHC were used to detect the the expressional changes of CNTF, then Western blot and RT-PCR were used to detect the expressional changes of STAT3、p-STAT3、CNTF and Caspase-9 in the ischemic segments of spinal cord.Results Compared with the sham group, in the SCIRI rats, the BBB scores were markedly decreased at all time points (P<0.01) , the latency and the amplitude of MEP and CSEP was longer and lower at 14 d post operation (P<0.01) , and this change was the most significant in the control group the second in the CNTF block group, and the last in the transplantation group, Resutts between each two groups were statistically significant (P<0.05) . At 7 d post operation, compared with the sham group, the immunoreactive products of CNTF were decreased in the CNTF block group (P<0.05) , but were increased (P<0.05) in the control group and the transplantation group (P<0.05) , and results in the transplantation group were higher than in the control group (P<0.05) . At 7 d post operation, compared with the sham group, the m RNA and protein level of CNTF、STAT3、 p-STAT3 were decreased obviously in CNTF block group (P<0.05) , the levels were increased in the control group and the transplantation group (P<0.05) , and the levels in the transplantation group were higher than that in the control group (P<0.05) ; but the m RNA and protein level of Caspase-9 were only decreased in the transplantation group (P<0.05) , the level was increased in the CNTF block group and the control groups (P<0.05) , and the level in the CNTF block group was more significantly increased than that in the control group (P<0.05) . At 14 d post operation, in CNTF block group, the m RNA and protein level of CNTF、STAT3、p-STAT3 were significantly higher than that in the sham group and the control group (P<0.05) , and the m RNA and protein level of caspase-9 was higher than that in the sham group (P<0.05) , but lower than that in the control group (P<0.05) , there were not statistically different in the level of each factor compared with transplantation group (P>0.05) . Conclusions BMSCs, transplanted by the abdominal aorta, can promote the expression of CNTF in the injuried spinal cord and significantly improve the hind limb function recovery by CNTF-mediated signaling pathway downstream of STAT3/Caspase-9 SCIRI in rats, but the role of BMSCs can be weakening by CNTF block that inhibited STAT3/Caspase-9 signaling pathway.
- Spinal cord,
- Ischemic-reperfusion injury,
- Abdominal aorta,
- Bone marrow mesenchymal stem cells (BMSCs) ,
- CNTF,
- STAT3

FullText(HTML)

References(20)

References

[1]	[1]SUN Y, TIAN Y, LI H, et al.Antinociceptive Effect of Intrathecal Injection of Genetically Engineered Human Bone Marrow Stem Cells Expressing the Human Proenkephalin Gene in a Rat Model of Bone Cancer Pain[J].Pain Res Manag, 2017, 2017 (1) :7346103.
[2]	[2]CHEN B K, STAFF N P, KNIGHT A M, et al.A safety study on intrathecal delivery of autologous mesenchymal stromal cells in rabbits directly supporting Phase I human trials[J].Transfusion, 2015, 55 (5) :1013-1020.
[3]	[3]UEDA S, SHIMASAKI M, ICHISEKI T, et al.Prevention of glucocorticoid-associated osteonecrosis by intravenous administration of mesenchymal stem cells in a rabbit model[J].BMC Musculoskelet Disord, 2017, 18 (1) :480.
[4]	[4]CUI LL, NITZSCHE F, PRYAZHNIKOV E, et al.Integrinα4 Overexpression on Rat Mesenchymal Stem Cells Enhances Transmigration and Reduces Cerebral Embolism After Intracarotid Injection[J].Stroke, 2017, 48 (10) :2895-2900.
[5]	[5]LUNDBERG J, SDERSTEN E, HOLMIN S, et al.Targeted intra-arterial transplantation of stem cells to the injured CNSis more effective than intravenous administration:engraftment is dependent on cell type and adhesion molecule expression[J].Cell Transplant, 2012, 21 (1) :333-343.
[6]	[6]LU D, LI Y, WANG L, et al.Intraarterial administration of marrow stromal cells in a rat model of traumatic brain injury[J].J Neurotrauma, 2001, 8 (8) :813-819.
[7]刘敬霞, 李建生, 刘轲, 等.脑脉通联合骨髓间充质干细胞移植对脑缺血大鼠神经营养因子表达的影响[J].时珍国医国药, 2013, 24 (8) :1986-1989.
[8]陈珊珊, 郭小兵, 金华.动脉移植BMSCs对大鼠缺血再灌注损伤脊髓CNTF和STAT3的影响[J].中风与神经疾病杂志, 2014, 31 (6) :492-496.
[9]陈珊珊, 宁杰, 朱丽璇, 等.肾下腹主动脉移植骨髓间充质干细胞治疗脊髓缺血再灌注损伤大鼠模型的建立[J].昆明理工大学学报, 2014, 39 (4) :99-104.
[10]	[10]BASSO D M, BEATTIE M S, BRESNAHAN J C.Descending systems contributing to locomotor recov-ery after mild or moderate spinal cord injury in rats:experimental evidence and a review of l-iterature[J].Restor Neurol Neurosci, 2002, 20 (5) :189-218.
[11]	[11]COPEMAN C A, CUZNER M L, GROOMEN N, et al.Temporal analysis of growth factor m RNA expression in myelinating rat brain aggregate cultures:increments in CNTF, FGF-2, IGF-I, and PDGF-AA m RNA are induced by antibody-mediated demyelination[J].Glia, 2000, 30 (4) :342-351.
[12]	[12]HELFAND S L, SMITH G A, WESSELLS N K.Survival and development in culture of dissociated parasympathetic neurons from ciliary ganglia[J].Dev Biol, 1976, 50 (2) :541-547.
[13]	[13]BARBIN G, MANTHORPE M, VARON S.Purification of the chick eye ciliary neurotrophie factor[J].J Neurochem, 1984, 43 (13) :1468-1478.
[14]习杨彦彬, 王廷华.CNTF基因在正常大鼠和猫脊髓组织中的表达[J].昆明医学院学报, 2006, 27 (6) :34-37.
[15]曾洪艳, 李力燕, 郭小兵.神经营养因子、凋亡相关因子和轴突导向因子在大鼠神经管畸形发育中的表达[J].昆明医科大学学报, 2012, 33 (11) :13-18.
[16]	[16]TRIPATHI RB, MCTIGUE DM.Chronically increased ciliary neurotrophic factor and fibroblast growth factor-2 expression after spinal contusion in rats[J].J Comp Neurol, 2008, 510 (2) :129-144.
[17]	[17]YETISER S, KAHRAMAN E.An analysis of time-dependent changes of neurotrophic factors (BDNF, CNTF) in traumatic facial nerve injury of a nerve-cut and nerve-crush model in rats[J].Otol Neurotol, 2008, 29 (3) :392-396.
[18]	[18]HOLST A, HELLER S, JUNGHANS D, et al.Onset of CNTFRalpha expression and signal transduction during neurogenesis in chick sensory dorsal root ganglia[J].Dev Biol, 1997, 191 (1) :1-13.
[19]	[19]SANTOS M D, YASUIKE M, KONDO H, et al.A novel type-1 cytokine receptor from fish involved in the Janus kinase/signal transducers and activators of transcription (Jak/STAT) signal path-way[J].Mol Immunol.2007, 44 (13) :3355-3363.
[20]陈珊珊, 金华.动脉移植BMSCs对缺血再灌注损伤大鼠细胞凋亡和Caspase-9基因的影响[J].基础医学与临床, 2015, 35 (7) :900-905.

Relative Articles(20)

Relative Articles

[1]	Yanping LI, Xiaolin¹ DONG, Qingyun LI, Hongmei LI, Huan WEI, Yi ZENG. miR-21-5p Alleviates OGD/R-induced Inflammation and Apoptosis of HT22 Cells and Promotes Proliferation by Inhibiting STAT3. Journal of Kunming Medical University, 2024, 45(12): 1-7.
[2]	Ruili XUE, Yuyin DUAN, Rui LIU, Xiaowei DU, Baihui YANG, Dong HUANG. Simultaneous Imaging of Ureter and Abdominal Aorta and Its Application in Open Surgery for Abdominal Aortic Aneurysm. Journal of Kunming Medical University, 2023, 44(6): 102-106. doi: 10.12259/j.issn.2095-610X.S20230623
[3]	Ke YANG, Junjie FEI, Yuyin DUAN, Xin LI, Zhongcheng WU, Yingshan HUANG. Establishment of Spinal Cord Ischemia Injury Model in Rats by Thoracic Aorta Occlusion Combined with Aortic Bypass. Journal of Kunming Medical University, 2022, 43(2): 18-22. doi: 10.12259/j.issn.2095-610X.S20220217
[4]	Wang Zeng Tao , Zhang Jie , Guo Tao . . Journal of Kunming Medical University, 2019, 40(04): 6-10.
[5]	Li Hong Yang , Su Tian Yi , Gong Jian Ping , Gan Ping , Yang Bu Rong , Cun Ying Li , Cha Yong , Zhao Gong Fang . . Journal of Kunming Medical University, 2018, 39(09): 11-14.
[6]	Zhao Yu , Yuan Jing , Liu Jian Jun , Lu Lin . Comparison of Proliferation between Fetal Forebrain-and Spinal Cord-Derived Neural Stem Cells in Mice. Journal of Kunming Medical University, 2017, 38(09): 19-23.
[7]	Song Shi Gang , Wu Di , Bai Ping . . Journal of Kunming Medical University, 2017, 38(02): 74-77.
[8]	Li Liu Zheng . The Experience of Surgical Treatment of Abdominal Aortic Aneurysm. Journal of Kunming Medical University,
[9]	Zhang Wei . Effect of SGK1 on the Protection of Ischemia Reperfusion Model Rats. Journal of Kunming Medical University,
[10]	Hu Yan Jie . . Journal of Kunming Medical University,
[11]	Zeng Hong Yan . . Journal of Kunming Medical University,
[12]	. Protecton Mechanism of Shuanghuanglian against Liver Ischemia/Reperfusion injury in Rats. Journal of Kunming Medical University,
[13]	. . Journal of Kunming Medical University,
[14]	. Treatment Experiences of Ulinstatin on the Reperfusion Injury of Postoperative Artery of Lower Extremity Acute Ischemia. Journal of Kunming Medical University,
[15]	. Specific Identification of Neurons and Astrocytes in Spinal Cords of Adult Monkeys and Rats. Journal of Kunming Medical University,
[16]	. A Preliminary Study on Magnetic Resonance Diffusion Imaging of Normal Canine Spinal Cord. Journal of Kunming Medical University,
[17]	. Expression of CNTF, TGF-β1, and IGF-1 in NSCs Cultured in vitro. Journal of Kunming Medical University,
[18]	Hu Yan Li . . Journal of Kunming Medical University,
[19]	Yin Lu Wei . . Journal of Kunming Medical University,
[20]	Cheng Jian Jun . . Journal of Kunming Medical University,