Construction of UV-induced Skin Squamous Cell Carcinoma Model of SKH-1 Hairless Mice and Expression of DNA-PKcs in Mice and Adult Squamous Cell Carcinoma

Jiang Hui; Zhang Juan; Xu Dan; He Li

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Jiang Hui , Zhang Juan , Xu Dan , He Li . Construction of UV-induced Skin Squamous Cell Carcinoma Model of SKH-1 Hairless Mice and Expression of DNA-PKcs in Mice and Adult Squamous Cell Carcinoma[J]. Journal of Kunming Medical University, 2019, 40(03): 11-17.

Citation:

Jiang Hui , Zhang Juan , Xu Dan , He Li . Construction of UV-induced Skin Squamous Cell Carcinoma Model of SKH-1 Hairless Mice and Expression of DNA-PKcs in Mice and Adult Squamous Cell Carcinoma[J]. Journal of Kunming Medical University, 2019, 40(03): 11-17.

Citation:

Jiang Hui , Zhang Juan , Xu Dan , He Li . Construction of UV-induced Skin Squamous Cell Carcinoma Model of SKH-1 Hairless Mice and Expression of DNA-PKcs in Mice and Adult Squamous Cell Carcinoma[J]. Journal of Kunming Medical University, 2019, 40(03): 11-17.

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Construction of UV-induced Skin Squamous Cell Carcinoma Model of SKH-1 Hairless Mice and Expression of DNA-PKcs in Mice and Adult Squamous Cell Carcinoma

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基金：国家自然科学基金资助项目 (81472540, 81660518); 昆明医科大学硕士研究生创新基金资助项目 (2018S092);

Received Date: 2018-11-06

Abstract

Abstract

Objective To construct an animal model of squamous cell carcinoma of the skin in SKH-1 mice with ultraviolet irradiation, and to investigate the DNA-PKcs activation level of DNA-PKcs-m TORC2/Akt signal transduction pathway during UV-induced skin SCC and AK to SCC transformation. Methods 43 SKH-l hairless mice were randomly divided into experimental group (33) and control group (10) .The experimental group used the SUV-1000 solar simulator to simulate sun-irradiated SKH-1 hairless mice, and the control group did not intervene. Experimental group 1 maintained irradiation for 24 weeks, and experimental group 2 maintained irradiation for 16 weeks. The mice were sacrificed at 12, 18, 24, and 28 weeks for skin histopathological examination. At 28 weeks, the same skin of the same mouse was taken for histopathological examination and immunohistochemistry.Five cases of adult solar keratosis (AK) , cutaneous squamous cell carcinoma (SCC) , normal light-protected site (NNS) , and normal exposed site (NES) were collected for Western Blot. Results After 12 weeks, some mice in the experimental group were thickened in the back skin. At 17 weeks, the papules with diameter ≥ 1 mm appeared in the back skin of experimental group. After 28 weeks of irradiation, the tumor formation rate reached100%. No tumor formation was observed in the control group. Both DNA-PKcs and p-DNA-PKcs (T2609) were localized in the cytoplasm and nucleus. Expression of DNA-PKcs increased in epidermal tissue. There was a difference in the statistical analysis of DNA-PKcs expression (P<0.05) , but there was no difference in the expression of p-DNA-PKcs (T2609) (P>0.05) .The expression levels of DNA-PKcs and p-DNA-PKcs (T2609) in human epidermal tissue increased and there were statistical differences (P<0.05) .Conclusions We successfulyl prepared of mouse AK model and SCC model. It is clear that UV-induced squamous cell carcinoma of the skin can induce DNA damage and repair.
- Ultraviolet radiation,
- Squamous cell carcinoma,
- Actinic keratosis,
- Disease models,
- Animal,
- DNA-PKcs

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References(14)

References

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