Researches on the Effect of Alcohol on Endogenous GHB in Human Blood by LC-MS/MS Method

Fang LUO; Ruibin ZHAO; Guojun LI; Xuxian FU; Xianmou FAN; Shaoming SU; Yunbo WEN; Qilin HUANG; Bin LI

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Fang LUO, Ruibin ZHAO, Guojun LI, Xuxian FU, Xianmou FAN, Shaoming SU, Yunbo WEN, Qilin HUANG, Bin LI. Researches on the Effect of Alcohol on Endogenous GHB in Human Blood by LC-MS/MS Method[J]. Journal of Kunming Medical University.

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Researches on the Effect of Alcohol on Endogenous GHB in Human Blood by LC-MS/MS Method

1.
Research Institution of Criminal Science and Technology of Kunming Public Security Bureau ，Kunming Yunnan 650500
2.
Yunnan Police College，Kunming Yunnan 650221
3.
College of Forensic Medicine，Kunming Medical University，Kunming Yunnan 650500，China

Received Date: 2024-01-12
Available Online: 2024-04-29

Abstract

Abstract

Objective Using LC-MS/MS method to study the effect of alcohol on endogenous GHB content in human blood . Methods To establish LC-MS/MS inspection method in the blood for γ- Hydroxybutyric acid （GHB）, γ- butyrolactone （GBL） and 1,4-butanediol （1,4-BD）. Taking 22 healthy subjects were selected as the research subjects and simulating real cases of drinking alcohol. Detecting the levels of GHB in their blood before and after drinking , and to compare the endogenous GHB levels in the blood before and after drinking. Results GHB in the concentration range of 0.1～50 μg/mL has a good linear relationship and there was a good linear relationship between GBL and 1,4-BD in the concentration range of 0.15～50 μg/mL （R² > 0.999）, The limits of detection of GHB, GBL and 1,4-BD were 0.015 μg/mL, 0.025 μg/mL and 0.01 μg/mL, respectively, and the limits of GHB, GBL and 1,4-BD quantification were 0.1 μg/mL, 0.15 μg/mL and 0.15 μg/mL. The matrix effects of the three compounds ranged from 18.2% to 20.1%, the recoveries were 81.6% to 92.3%, the intra-day and inter-day precision of RSD were less than 10%, and the accuracy was -5.8%-7.0%. The content of GHB in the blood after 7 hours of drinking were higher than that before drinking alcohol （P < 0.05）, and the concentration range was 0.71-3.81 μg/mL. Conclusion The content of endogenous GHB in human blood has increased after 7 hours of drinking alcohol, but the concentration was lower than 4 μg/mL, which provided data support for the assessment of endogenous and exogenous GHB involved in real cases, enriching the forensic toxicology data of GHB .
- γ-hydroxybutyric acid,
- γ-Butyrolactone,
- 1,4-Butanediol,
- Alcohol,
- LC-MS/MS method

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References(17)

References

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