Abstract: ［Abstract］Objective　To investigate the relationship between metalloproteinase-9 -9 （Matrix）， inhibitor-1 -1（MMP-9），metalloproteinase TIMP-1 factor（Matrix） and endothelial growth （VEGF）， and to investigate the correlation between the expression of（matrix） and vascular endothelial growth factor （endothelial）and vascular endothelial growth factor（vascular）. Methods　Sixty-eight cases of non small cell lung cancer patients treated in fengning manchu autonomous county hospital from January to January 2014 were analyzed retrospectively， and the expression level of TIMP-1， VEGF and MMP-9 were analyzed by immunohistochemical method. Results The positive rates of MMP-9（75%），TIMP-1（19.12%）and VEGF （13.24%）were significantly higher than those in normal tissues（P＜0.05），MMP-9 （60.87%），VEGF （54.55%），TIMP-1 （65.22%） and VEGF （76.09%， 59.09%，63.64%）. The positive rates of TIMP-1，VEGF and MMP-9 were higher than those without lymph node metastasis（P＜0.05）. Conclusion　MMP-9，TIMP-1 and VEGF expression levels can predict the invasion and metastasis of non small cell lung cancer，and the three kinds of substances can promote the development and progression of lung cancer in different degrees.