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富血小板血浆促进人子宫内膜间充质干细胞(EnMSCs)增殖的机制

王晓寒 牟善茂 郝翠芳 任琳琳 王敏 赵彤

王晓寒, 牟善茂, 郝翠芳, 任琳琳, 王敏, 赵彤. 富血小板血浆促进人子宫内膜间充质干细胞(EnMSCs)增殖的机制[J]. 昆明医科大学学报, 2022, 43(1): 26-32. doi: 10.12259/j.issn.2095-610X.S20220126
引用本文: 王晓寒, 牟善茂, 郝翠芳, 任琳琳, 王敏, 赵彤. 富血小板血浆促进人子宫内膜间充质干细胞(EnMSCs)增殖的机制[J]. 昆明医科大学学报, 2022, 43(1): 26-32. doi: 10.12259/j.issn.2095-610X.S20220126
Xiaohan WANG, Shanmao MOU, Cuifang HAO, Linlin REN, Min WANG, Tong ZHAO. Platelet-rich Plasma Promotes the Proliferation of Human Endometrial Mesenchymal Stem Cells (EnMSCs) through the PI3K/AKT/mTOR Signaling Pathway[J]. Journal of Kunming Medical University, 2022, 43(1): 26-32. doi: 10.12259/j.issn.2095-610X.S20220126
Citation: Xiaohan WANG, Shanmao MOU, Cuifang HAO, Linlin REN, Min WANG, Tong ZHAO. Platelet-rich Plasma Promotes the Proliferation of Human Endometrial Mesenchymal Stem Cells (EnMSCs) through the PI3K/AKT/mTOR Signaling Pathway[J]. Journal of Kunming Medical University, 2022, 43(1): 26-32. doi: 10.12259/j.issn.2095-610X.S20220126

富血小板血浆促进人子宫内膜间充质干细胞(EnMSCs)增殖的机制

doi: 10.12259/j.issn.2095-610X.S20220126
基金项目: 山东省医药卫生科技发展计划基金资助项目(202005031192)
详细信息
    作者简介:

    王晓寒(1983~),女,山东泰安人,医学博士,主治医师,主要从事子宫内膜容受性及女性生殖医学研究工作

    通讯作者:

    赵彤,E-mail: zhaotong667@163.com

  • 中图分类号: R714

Platelet-rich Plasma Promotes the Proliferation of Human Endometrial Mesenchymal Stem Cells (EnMSCs) through the PI3K/AKT/mTOR Signaling Pathway

  • 摘要:   目的   探讨PRP促进EnMSCs增殖的机制,为EnMSCs的扩增及临床应用提供理论基础。   方法   将EnMSCs随机分为对照组、2%PRP组、2%PRP + LY294002组,CCK-8法检测细胞增殖情况;流式细胞仪监测细胞周期及Western Blot和ELISA检测细胞中p-PI3K、AKT、p-AKT、mTOR及p-mTOR的蛋白表达。   结果   (1) CCK-8结果显示:与对照组相比较,2%PRP组EnMSCs的增殖水平显著较高(P < 0.05);与2%PRP组相比,2%PRP+LY294002组EnMSCs的增殖水平显著较低( P < 0.05);(2)流式细胞仪分析细胞周期结果显示:2%PRP组G2/M期细胞比例显著高于对照组和2%PRP+ LY294002组,G0/G1细胞比例明显低于对照组和2%PRP+ LY294002组,差异均有统计学意义( P < 0.05);(3) 2%PRP组EnMSCs中p-PI3K、AKT、p-AKT、mTOR、p-mTOR的蛋白表达明显高于对照组及2%PRP+LY294002组,差异均有统计学意义( P < 0.05)。   结论   EnMSCs的增殖由PI3K/AKT/ mTOR信号通路调控,PRP通过促进AKT、mTOR的蛋白表达及其磷酸化以激活该通路,促进EnMSCs由G1期向G2期转变,从而促进EnMSCs的增殖。
  • 图  1  各组48h EnMSCs生长情况(×200)

    A:对照组;B:2%PRP组;C:2%PRP + LY294002组

    Figure  1.  The 48 h EnMSCs proliferation phenomena for each group (×200)

    图  2  3组CCK-8增殖结果比较

    与对照组比较,**P < 0.01。

    Figure  2.  The CCK-8 proliferation results comparison between the three groups

    图  3  各组流式细胞周期及细胞周期比例图

    A:各组流式细胞周期图;B:细胞周期比例图。

    Figure  3.  The flow cell cycle and cell cycle proportion gram in three groups

    图  4  3组p-PI3K、AKT、p-AKT、mTOR、p-mTOR蛋白表达水平比较

    A:Western blot结果;B:ELISA结果。

    Figure  4.  The p-PI3K, AKT, p-AKT, mTOR, and p-mTOR proteins demonstration levels comparison between the three groups

    表  1  3组EnMSCs细胞周期比例(%)比较(n = 3)

    Table  1.   The EnMSCs cell cycle proportion comparison in the three groups (n = 3)

    组别 G1/G0(%) S(%) G2/M(%)
    对照组 70.04 ± 2.67 25.49 ± 2.68 4.47 ± 1.04
    2%PRP组 54.63 ± 4.23**▲ 30.66 ± 2.85*▲ 14.71 ± 2.72**▲
    2%PRP+ LY294002组 72.16 ± 4.26 17.45 ± 3.49** 10.39 ± 2.84**
      与对照组比较,*P < 0.05, **P < 0.01;与2%PRP+ LY294002组比较, P < 0.01。
    下载: 导出CSV
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  • 收稿日期:  2021-12-18
  • 刊出日期:  2022-01-25

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