<styled-content style-type="number">1800</styled-content> MHz电磁辐射对3xTg-AD和C57小鼠的学习及认知功能影响

徐晓霜; 马国玉; 和润华; 赵永丽; 武慧欣; 木云珍

1800 MHz电磁辐射对3xTg-AD和C57小鼠的学习及认知功能影响

1.
昆明医科大学公共卫生学院，云南昆明　650500
2.
云南省肿瘤医院/昆明医科大学第三附属医院/北京大学肿瘤医院云南医院肿瘤防治办公室，云南昆明　650118
3.
云南省煤田地质局综合管理处，云南昆明　650034
4.
丽江市疾病预防控制中心公共卫生科，云南丽江　674100

基金项目: 云南省科技厅-昆明医科大学应用基础研究联合专项基金（202101AY070001-056；202101AY070001-057）

详细信息

作者简介:
徐晓霜（1999～），女，重庆大足人，医学硕士，主要从事公共卫生研究工作

马国玉与徐晓霜对本文有同等贡献

通讯作者:
木云珍，E-mail：muyz98@163.com

中图分类号: R594.8
计量
- 文章访问数: 46
- HTML全文浏览量: 32
- PDF下载量: 3
- 被引次数: 0
出版历程
- 收稿日期: 2024-08-26
- 网络出版日期: 2025-01-06

The Effect of 1800 MHz Electromagnetic Radiation on Learning and Cognitive Functions in 3xTg-AD Mice

1.
School of Public Health，Kunming Medical University，Kumming Yunnan 650500
2.
Department of Cancer Prevention and Control，Yunnan Cancer Hospital，The Third Affiliated Hospital of Kunming Medical University，Peking University Cancer Hospital Yunnan，Kunming Yunnan 650118
3.
Comprehensive Management Department of Yunnan Coalfield Geological Bureau，Kunming Yunnan 650034
4.
Public Health Department，Lijiang Center for Disease Control and Prevention，Lijiang Yunnan 674100，China

摘要

摘要: 目的探讨1800 MHz电磁辐射（electromagnetic radiation，EMR）对3xTg-AD小鼠和57C小鼠认知功能的影响，为电磁辐射对人体的潜在影响提供理论依据。方法以12只3xTg-AD转基因小鼠和12只野生型C57小鼠为研究对象，将1月龄的小鼠分为四组：RF WT（C57小鼠暴露组）、Control WT（C57小鼠对照组）、RF AD（3xTg-AD小鼠暴露组）、Control AD（3xTg-AD小鼠对照组），每组6只。每晚20:00至次日8:00进行1800 MHz EMR暴露实验，持续5个月。暴露结束后进行水迷宫测试，评估EMR对3xTg-AD小鼠空间学习和记忆能力的影响，并测量体重、大脑重量及计算脑体比，最后采用Western Blot技术测量海马组织中APP、NR1、NR2A指标，分析1800 MHz EMR对3xTg-AD小鼠认知功能的影响。结果在1800 MHz EMR下，四组间的Morris水迷宫空间学习能力差异无统计学意义（P > 0.05），RF AD组的逃逸时间、游泳路程长度和穿越目标象限次数高于其他组（P < 0.05）。Western Blot结果显示，3xTg-AD小鼠的APP蛋白表达高于C57小鼠（P < 0.05）。WT组的NR1蛋白表达高于AD组（P < 0.05）；AD组中，RF AD组高于Control WT组（P < 0.05），Control WT组的NR2A蛋白高于其他组（P < 0.05）。结论长时间1800 MHz EMR暴露会影响3xTg-AD及C57小鼠学习与认知功能。
- 电磁辐射 /
- 3xTg-AD小鼠 /
- 淀粉样前体蛋白 /
- N-甲基-D-天冬氨酸受体1 /
- N-甲基-D-天冬氨酸受体2A
Abstract: Objective To Explore the effects of 1800 MHz electromagnetic radiation （EMR） on cognitive function of 3xTg-AD and 57C mice, providing a theoretical basis for the potential impacts of electromagnetic radiation on the human body. Methods A total of 12 3xTg-AD transgenic mice and 12 wild-type C57 mice were selected as research subjects. The one-month-old mice were divided into four groups: RF WT, Control WT, RF AD, and Control AD, with 6 mice in each group.The 1800 MHz EMR exposure experiments were conducted from 20:00 to 8:00 the next day for a duration of 5 months. After the exposure, a water maze test was conducted to evaluate the effects of EMR on spatial learning and memory abilities of 3xTg AD mice. along with measurements of body weight, brain weight, and calculation of the brain-to-body ratio. Finally, Western Blot technique was used to measure the levels of APP, NR1, and NR2A in hippocampal tissue to analyze effects of 1800 MHz EMR on the cognitive function of 3xTg AD mice. Results Under 1800 MHz EMR exposure, there were no statistically significant differences in Morris water maze spatial learning ability among the four groups （P > 0.05）. However, longer escape time, greater swimming distances, and more crossings of target quadrant were exhibited in the RF AD group compared to the other groups （P < 0.05）. Western Blot results showed that the APP protein levels in 3xTg AD mice was higher than those in C57 mice （P < 0.05）. The expression levels of NR1 protein in the WT group was higher than those in the AD group （P < 0.05）; In the AD group, the RF AD group was higher levels than the Control WT group （P < 0.05）, and the NR2A protein levels in the Control WT group were higher than in the other groups （P < 0.05）. Conclusion Prolonged exposure to 1800 MHz EMR can affect the learning and cognitive function of both 3xTg AD and C57 mice.
- Electromagnetic radiation /
- 3xTg-AD mice /
- Amyloid precursor protein /
- N-methyl-D-aspartate receptor 1 /
- N-methyl-D-aspartate receptor 2A

HTML全文

图 1 暴露后六月龄小鼠水迷宫定位续航实验

A：暴露后逃避潜伏期；B：暴露后总路径长度；C：距离目标的矢量距离；D：游泳速度；E：Control AD组水迷宫轨迹图；F：RF AD组水迷宫轨迹图；G：Control WT组水迷宫轨迹图；H：RF WT组水迷宫轨迹图。

Figure 1. Morris water maze navigation and endurance test for six-month-old mice post-exposure

下载: 全尺寸图片幻灯片

图 2 APP、NR1、NR2A组织蛋白表达

A：APP、NR1、NR2A组织WB条带；B：APP蛋白相对表达量C：NR1蛋白相对表达量；D：NR2A蛋白相对表达量。^*P<0.05，^**P<0.01，^***P<0.001。

Figure 2. Expression of APP，NR1，and NR2A proteins in tissues

下载: 全尺寸图片幻灯片

表 1 实验鼠的基本情况[M（P₂₅，P₇₅）/（$\bar x \pm s $）]

Table 1. Basic information of experimental mice[M（P₂₅，P₇₅）/（$ \bar x \pm s$）]

组别	体重（g）	大脑质量（g）	脑体比（‰）
Control AD组（n=6）	12.61（9.15，14.61）	0.30±0.03	0.012（0.009，0.015）
RF AD组（n=6）	13.06（11.59，13.75）	0.30±0.05	0.013（0.011，0.014）
Control WT组（n=6）	14.08（12.73，15.97）	0.32±0.03	0.014（0.012，0.016）
RF WT组（n=6）	14.81（14.38，15.24）	0.32±0.02	0.015（0.014，0.016）
F/H值	8.505	1.038	8.552
P值	0.032^*	0.389	0.036^*
^*P<0.05。

下载: 导出CSV

表 2 小鼠水迷宫空间探索实验结果（$ \bar x \pm s $）

Table 2. Results of morris water maze spatial exploration test for mice （$ \bar x \pm s $）

组别	EL（S）	TPL（cm）	SS（S）	T3/Tt	D3/Dt	TC（次）
Control AD组（n=6）	3.69±2.11	1015.19±85.68	17.14±1.52	0.31±0.10	0.31±0.10	2.00±0.00
RF AD组（n=6）	12.29±3.24	1210.87±65.79	19.90±1.47	0.30±0.03	0.32±0.03	3.33±0.58
Control WT组（n=6）	2.99±0.96	980.43±97.64	18.07±2.50	0.27±0.10	0.29±0.08	2.25±0.50
RF WT组（n=6）	3.79±0.37	1036.08±36.97	17.63±0.30	0.28±0.07	0.29±0.05	1.67±0.58
F/H值	16.590	5.539	1.406	0.116	0.164	6.748
P值	0.001^*	0.020^*	0.303	0.949	0.918	0.011^*
^*P < 0.05。

下载: 导出CSV

参考文献(17)

[1]	Sundaram V,Mohammed S,Zyuzikov N. Effects of 150 kHz intermediate frequency electromagnetic radiation on fertility indicators in male rats[J]. Heliyon,2022,8(12):122-143.
[2]	中华人民共和国国家统计局. 2022年国民经济和社会发展统计公报[J]. 中国统计,2023,1(3):12-29.
[3]	Russell C L. 5 G wireless telecommunications expansion: Public health and environmental implications[J]. Environ Res,2018,165(5):484-495.
[4]	Banik S,Bandyopadhyay S,Ganguly S. Bioeffects of microwave:A brief review [J]. Bioresour Technol,2003,87(2): 155-164.
[5]	Lagorio S,Blettner M,Baaken D,et al. The effect of exposure to radiofrequency fields on cancer risk in the general and working population: A protocol for a systematic review of human observational studies[J]. Environment International,2021,157(4):238-247.
[6]	Jayan J,Roshi H,Ashraf F,et al. Effects of radiation exposure on brain health: A state of the art and new challenges[J]. Environmental Science and Pollution Research International,2022,29(58):87068-87081. doi: 10.1007/s11356-022-23703-4
[7]	Sommer A,Streckert J,Bitz A,et al. No effects of GSM-modulated 900 MHz electromagnetic fields on survival rate and spontaneous development of lymphoma in female AKR/J mice[J]. BMC Cancer,2004,4(2):772-789.
[8]	Ferguson S,Sarkar S,Schmued L. Longitudinal behavioral changes in the APP/PS1 transgenic Alzheimer's disease model[J]. Behavioural Brain Research,2013,242(2):125-134.
[9]	Bouji M,Lecomte A,Gamez C,et al. Neurobiological effects of repeated radiofrequency exposures in male senescent rats[J]. Biogerontology,2016,17(5-6):841-857. doi: 10.1007/s10522-016-9654-8
[10]	Zhao J,Liu X,Xia W,et al. Targeting Amyloidogenic processing of APP in Alzheimer's disease[J]. Frontiers in Molecular Neuroscience,2020,13(2):137-148.
[11]	Delport A,Hewer R. The amyloid precursor protein: a converging point in Alzheimer's disease[J]. Molecular Neurobiology,2022,59(7):4501-4516. doi: 10.1007/s12035-022-02863-x
[12]	Park J,Kwon J,Kim N,et al. Effects of 1950 MHz radiofrequency electromagnetic fields on Aβ processing in human neuroblastoma and mouse hippocampal neuronal cells[J]. Journal of Radiation Research,2018,59(1):18-26. doi: 10.1093/jrr/rrx045
[13]	Owlett L,Belcher E,Dionisio-Santos D,et al. Space radiation does not alter amyloid or tau pathology in the 3xTg mouse model of Alzheimer's disease[J]. Life Sciences in Space Research,2020,27(2):89-98.
[14]	Dainauskas J,Marie H,Migliore M,et al. GluN2B-NMDAR subunit contribution on synaptic plasticity: A phenomenological model for CA3-CA1 synapses[J]. Frontiers in synaptic neuroscience,2023,15(4):1113-1148.
[15]	Hajdú T,Juhász T,Szűcs-Somogyi C,et al. NR1 and NR3B composed intranuclear N-methyl-d-aspartate receptor complexes in human melanoma cells[J]. International Journal of Molecular Sciences,2018,19(7):134-159.
[16]	Furuie H,Yamada M. Neonatal blockade of NR2A-containing but not NR2B-containing NMDA receptor induces spatial working memory deficits in adult rats[J]. Neuroscience Research,2022,176(4):57-65.
[17]	袁海阳,朱梦姚,王祎,等. 保元汤对于记忆损伤小鼠的行为学以及海马体蛋白的影响[J]. 四川中医,2023,41(9):66-72.

相关文章(20)

[1]	徐晓霜, 熊庆, 张媛, 武慧欣, 和丽梅, 木云珍. 1800 MHz电磁辐射暴露对大鼠海马GFAP表达的“窗口效应”, 昆明医科大学学报. 2024, 45(3): 30-34. doi: 10.12259/j.issn.2095-610X.S20240304
[2]	马葳, 李梅华, 蒋菁, 刘兴许, 杨丽芬, 李丽. 肝素结合蛋白及血清淀粉样蛋白A在AECOPD合并肺部感染中的研究进展, 昆明医科大学学报. 2023, 44(2): 172-176. doi: 10.12259/j.issn.2095-610X.S20230228
[3]	吴锡芳, 汪勇, 彭丽莎, 罗洁, 王枫. 血管内皮生长因子-C对鼻咽癌CNE-2细胞小鼠移植瘤的辐射敏感性的影响, 昆明医科大学学报. 2022, 43(12): 6-11. doi: 10.12259/j.issn.2095-610X.S20221205
[4]	牛玲, 李博一, 张程, 马蓉, 唐艳, 刘方, 尹利民, 韩竺君, 苗翠娟, 张娴. 降钙素受体、维生素D受体基因多态性与昆明地区2型糖尿病合并骨质疏松的关系, 昆明医科大学学报. 2021, 42(11): 74-80. doi: 10.12259/j.issn.2095-610X.S20211114
[5]	张盛庆宇, 舒逍, 吴锡南, 李志强, 武慧欣, 张媛, 蒋玉融, 杨思佳, 木云珍. 1 800 MHz射频电磁场暴露对大鼠海马GFAP、NCAM和GABA受体表达的影响, 昆明医科大学学报. 2021, 42(9): 13-19. doi: 10.12259/j.issn.2095-610X.S20210939
[6]	任朝凤, 刘芬, 杨丽芬, 吴涛, 赵娅琳. 肺动脉血栓栓塞患者检测血栓弹力图、D-二聚体及纤维蛋白原的相关性, 昆明医科大学学报. 2020, 41(02): 96-99.
[7]	郭玉倩, 曾跃勤, 吴超, 陆姜利, 杨艺, 郑红, 梁张. Aβ_25-35联合D-半乳糖诱导树鼩AD模型的建立, 昆明医科大学学报. 2020, 41(02): 21-25.
[8]	赵瑜, 赵安琪, 陈晨, 王琳琳. 视神经损伤后JNK3、APP蛋白的表达变化对视网膜神经节细胞存活的影响, 昆明医科大学学报. 2018, 39(02): 25-29.
[9]	袁英, 李渊渊, 郭方圆, 杨宏英. D-二聚体监测及低分子肝素钠的预防在妇科术后静脉血栓性疾病的应用, 昆明医科大学学报. 2017, 38(04): 109-112.
[10]	李艳明, 郑艳珍, 张冬先, 李娟, 杨根梦, 何永旺, 周一卿, 曾晓锋, 李桢. 甲基苯丙胺与HIV-Tat蛋白协同致大鼠纹状体NMDA受体NR2B亚基和iNOS的表达, 昆明医科大学学报. 2017, 38(07): 1-6.
[11]	温杰, 杜三军, 王晓美, 吴远慧, 安国芝, 霍岩. D-二聚体水平检测对过敏性紫癜患者的临床诊疗价值, 昆明医科大学学报. 2017, 38(03): 90-93.
[12]	贾亮. 血浆纤维蛋白原及D-二聚体监测对进展性脑卒中的诊断价值, 昆明医科大学学报. 2016, 37(02): -.
[13]	隋念含. 甲基苯丙胺依赖大鼠纹状体RGS4和D2受体信号传导通路mRNA的表达, 昆明医科大学学报. 2015, 36(12): -.
[14]	雷春莲. PIH患者血清D-二聚体及内皮素与脂肪因子水平及相关性研究, 昆明医科大学学报. 2014, 35(06): -.
[15]	卯建. 降钙素原、超敏C-反应蛋白和淀粉样蛋白检测在细菌性感染诊断中的应用价值, 昆明医科大学学报. 2013, 34(07): -.
[16]	黄晓斌. 伽玛刀治疗吗啡依赖猴双侧伏隔核后多巴胺D3受体变化的研究, 昆明医科大学学报. 2013, 34(06): -.
[17]	冯倩. 全自动生化分析仪测定D-二聚体的分析性能评价, 昆明医科大学学报. 2013, 34(02): -.
[18]	张媛. 1 800 MHz电磁辐射对大鼠皮肤组织氧化应激的影响, 昆明医科大学学报. 2013, 34(05): -.
[19]	顾玉. 同型半胱氨酸对小鼠骨骼肌组织PDK1的影响, 昆明医科大学学报. 2012, 33(02): -.
[20]	. 腹腔"熟鸡蛋"样游离体1例, 昆明医科大学学报. 2011, 32(05): -.