Effect of Shen-ling-bai-zhu-san on Methamphetamine-Induced Gut Microbiota Change in Mice

Lin XU; Kaicheng GAO; Jie JIA; Yuyang LI; Huawei WANG; Yiqun KUANG; Yu ZHAO

doi:10.12259/j.issn.2095-610X.S20230829

Volume 44 Issue 8

Aug. 2023

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Article Navigation > Journal of Kunming Medical University > 2023 > 44(8): 37-43

Lin XU, Kaicheng GAO, Jie JIA, Yuyang LI, Huawei WANG, Yiqun KUANG, Yu ZHAO. Effect of Shen-ling-bai-zhu-san on Methamphetamine-Induced Gut Microbiota Change in Mice[J]. Journal of Kunming Medical University, 2023, 44(8): 37-43. doi: 10.12259/j.issn.2095-610X.S20230829

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Lin XU, Kaicheng GAO, Jie JIA, Yuyang LI, Huawei WANG, Yiqun KUANG, Yu ZHAO. Effect of Shen-ling-bai-zhu-san on Methamphetamine-Induced Gut Microbiota Change in Mice[J]. Journal of Kunming Medical University, 2023, 44(8): 37-43. doi: 10.12259/j.issn.2095-610X.S20230829

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Effect of Shen-ling-bai-zhu-san on Methamphetamine-Induced Gut Microbiota Change in Mice

doi: 10.12259/j.issn.2095-610X.S20230829

Lin XU¹,
Kaicheng GAO¹,
Jie JIA¹,
Yuyang LI^{1, 2},
Huawei WANG²,
Yiqun KUANG^{1
,
,},
Yu ZHAO^{1
,
,}

1.
Research Center for Clinical Medicine，First Affiliated Hospital of Kunming Medical University
2.
Dept. of Reproduction and Genetics，First Affiliated Hospital of Kunming Medical University，Kunming Yunnan 650032，China

Received Date: 2023-06-23
Available Online: 2023-09-09
Publish Date: 2023-08-30

Abstract

Abstract

Objective To explore the effect and regulatory mechanism of Shen-ling-bai-zhu-san （SLBZS）, a traditional Chinese medicine, on methamphetamine （METH）-induced gut microbiota change in mice. Methods SPF C57BL/6J mice were divided into three groups: saline group, METH group, and SLBZS+METH group, the latter two groups of mice were intraperitoneal injected METH for three weeks and intragastric administration of SLBZS for one week after METH injection, the immunological and biochemical indexes of mice peripheral blood were examined, and intestinal fecal samples of mice were collected for 16S rRNA sequencing, and then subjected to bioinformatic analysis. Results Compared to METH group, the percentage and number of white blood cells and lymphocytes were significantly increased （ P < 0.05）, while the number of neutrophils decreased in SLBZS+METH group （P < 0.05）, which indicated that SLBZS can enhance immunity and reduce the inflammatory response of METH-treated mice. The data of 16S rRNA sequencing indicated that the number of Operational Taxonomic Unit （OTU） of gut microbiota decreased in the METH group, which was characterized by the presence of pathogenic bacteria including Proteus mirabilis, Bittarella_massiliensis, Acinetobacter calcoacetate, etc. SLBZS administration increased the OTU number of gut microbiota, functional abundance of metabolism related to carbohydrates, cofactors, vitamins, amino acids, cell activity, and environmental adaptation. Conclusion SLBZS improves the immune response and the composition and function of gut microbiota in METH-treated mice.
- Shen-ling-bai-zhu-san,
- Methamphetamine,
- Gut microbiota

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References(23)

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